Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/45120
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dc.contributor.authorChen, L.R.en_US
dc.contributor.author陳志峰zh_TW
dc.contributor.authorChao, C.H.en_US
dc.contributor.authorChen, C.F.en_US
dc.contributor.authorLee, Y.P.en_US
dc.contributor.authorChen, Y.L.en_US
dc.contributor.authorShiue, Y.L.en_US
dc.date2007zh_TW
dc.date.accessioned2014-06-06T08:14:29Z-
dc.date.available2014-06-06T08:14:29Z-
dc.identifier.issn0378-4320zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/45120-
dc.description.abstractExpression levels of 33 high egg production candidate transcripts in Red-feather Taiwan country chickens (TCCs) were examined by quantitative reverse-transcription (RT) polymerase chain reactions (PCR) in this study. Candidate transcripts were previously identified from a L2-B (L2-subtract-B) hypothalamus/pituitary gland subtractive cDNA library. In this subtractive cDNA library, two divergently selected strains of TCCs, B and L2 were used. These two strains were originated from one single population and were further subjected (since 1982) to the selections of body weight/comb size (B) and eggs to 40 wk of age (L2), respectively. Hypothalamuses and pituitary glands that sampled from Red-feather TCCs were previously grouped into high (Red-high; n = 20) and low (Red-low-, it = 20) egg productions based on the rate of lay after 1st egg (hen-day laying rate; %). Rates of lay after 1st egg (mean +/- S.E.) in the Red-high and the Red-low sub-populations were 72.2 +/- 0.6 and 23.0 +/- 3.5, respectively (P < 0.01). Quantitative RT-PCR validated that 25 candidate transcripts were significantly higher expressed in the Red-high than in the Red-low hens. These transcripts were ANP32A, BDH, CDC42, CNTN1, COMT, CPE, CTNNB1, DIO2, EIF4E, GARNL1, HSPCA, LAPTM4B, MBP, NAPIL4, NCAM1, PARK7, PCDHA@, PGDS, PLAG1, PRL, RAD21, SARIA, SCG2, STMN1 and UFM1. Among these transcripts, 15 (79.0%), 13 (68.4%), and 12 (63.2%) genes were annotated to involve in cellular physiological process (GO:0050875), metabolism (GO:0008152) and cell communication (GO:0007154). Identified transcripts that related to high egg production are most active in focal adhesion, adherens junction, MAPK signaling, tight junction and cell adhesion pathways. (C) 2006 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationAnimal Reproduction Scienceen_US
dc.relation.ispartofseriesAnimal Reproduction Science, Volume 100, Issue 1-2, Page(s) 172-185.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.anireprosci.2006.07.005en_US
dc.subjectchickenen_US
dc.subjecthigh egg productionen_US
dc.subjecthypothalamusen_US
dc.subjectpituitary glanden_US
dc.subjecttranscriptsen_US
dc.subjectquantitative RT-PCRen_US
dc.subjecthen gallus-domesticusen_US
dc.subjectluteinizing-hormoneen_US
dc.subjectovulatory cycleen_US
dc.subjectprolactinen_US
dc.subjectprogesteroneen_US
dc.subjectperformanceen_US
dc.subjectsecretionen_US
dc.subjectproteinsen_US
dc.subjectdatabaseen_US
dc.subjectreleaseen_US
dc.titleExpression of 25 high egg production related transcripts that identified from hypothalamus and pituitary gland in red-feather Taiwan country chickensen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.anireprosci.2006.07.005zh_TW
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