Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/45131
標題: Identification of transcripts related to high egg production in the chicken hypothalamus and pituitary gland
作者: Shiue, Y.L.
陳志峰
Chen, L.R.
Chen, C.F.
Chen, Y.L.
Ju, J.P.
Chao, C.H.
Lin, Y.P.
Kuo, Y.M.
Tang, P.C.
Lee, Y.P.
唐品琦
關鍵字: chickens
egg production
hypothalamus
pituitary gland
transcriptomics
prostaglandin-d synthase
secretogranin-ii
chromogranin-a
catalytic-properties
gene-expression
prolactin prl
lh-release
cells
d-2
secretion
期刊/報告no:: Theriogenology, Volume 66, Issue 5, Page(s) 1274-1283.
摘要: To identify transcripts related to high egg production expressed specifically in the hypothalamus and pituitary gland of the chicken, two subtracted cDNA libraries were constructed. Two divergently selected strains of Taiwan Country Chickens (TCCs), B (sire line) and L2 (dam line) were used; they had originated from a single population and were further subjected (since 1982) to selection for egg production to 40 wk of age and body weight/comb size, respectively. A total of 324 and 370 clones were identified from the L2-B (L2-subtract-B) and the B-L2 subtracted cDNA libraries, respectively. After sequencing and annotation, 175 and 136 transcripts that represented 53 known and 65 unknown non-redundant sequences were characterized in the L2-B subtracted cDNA library. Quantitative reverse-transcription (RT)-PCR was used to screen the mRNA expression levels of 32 randomly selected transcripts in another 78 laying hens from five different strains. These strains included the two original strains (13 and L2) used to construct the subtracted cDNA libraries and an additional three commercial strains, i.e., Black- and Red-feather TCCs and Single-Comb White Leghorn (WL) layer. The mRNA expression levels of 16 transcripts were significantly higher in the L2 than in the B strain, whereas the mRNA expression levels of nine transcripts, BDH, NCAM1, PCDHA@, PGDS, PLAG1, PRL, SAR1A, SCG2 and STMN2, were significantly higher in two high egg production strains, L2 and Single-Comb WL; this indicated their usefulness as molecular markers of high egg production. (c) 2006 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/45131
ISSN: 0093-691X
文章連結: http://dx.doi.org/10.1016/j.theriogenology.2006.03.037
Appears in Collections:動物科學系

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