Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/45147
標題: Cell adhesion molecule Echinoid associates with unconventional myosin VI/Jaguar motor to regulate cell morphology during dorsal closure in Drosophila
作者: Lin, H.P.
黃三元
Chen, H.M.
Wei, S.Y.
Chen, L.Y.
Chang, L.H.
Sun, Y.J.
Huang, S.Y.
Hsu, J.C.
關鍵字: echinoid
jaguar
myosin VI
cell adhesion
cell migration
dorsal
closure
Drosophila
epithelial morphogenesis
signaling pathway
actin dynamics
adherens
junctions
vi
expression
protein
jnk
cadherin
kinase
期刊/報告no:: Developmental Biology, Volume 311, Issue 2, Page(s) 423-433.
摘要: Echinoid (Ed) is a homophilic immunoglobulin domain-containing cell adhesion molecule (CAM) that localizes to adherens junctions (AJs) and cooperates with Drosophila melanogaster epithelial (DE)-cadherin to mediate cell adhesion. Here we show that Ed takes part in many processes of dorsal closure, a morphogenetic movement driven by coordinated cell shape changes and migration of epidermal cells to cover the underlying amnioserosa. Ed is differentially expressed, appearing in epidermis but not in amnioserosa cells. Ed functions independently from the JNK signaling pathway and is required to regulate cell morphology, and for assembly of actomyosin cable, filopodial protrusion and coordinated cell migration in dorsal-most epidermal cells. The effect of Ed on cell morphology requires the presence of the intracellular domain (Ed(intra)). Interestingly, Ed forms homodimers in vivo and Ed(intra) monomer directly associates with unconventional myosin VI/Jaguar (Jar) motor protein. We further show that ed genetically interacts with jar to control cell morphology. It has previously been shown that myosin VI is monomeric in vitro and that its dimeric form can associate with and travel processively along actin filaments. Thus, we propose that Ed mediates the dimerization of myosin VI/Jar in vivo which in turn regulates the reorganization and/or contraction of actin filaments to control changes in cell shape. Consistent with this, we found that ectopic ed expression in the amnioserosa induces myosin VI/Jar-dependent apical constriction of this tissue. (c) 2007 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/45147
ISSN: 0012-1606
文章連結: http://dx.doi.org/10.1016/j.ydbio.2007.08.043
Appears in Collections:動物科學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.