Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/45156
標題: Changes in number of granulosa cells, follicular fluid levels and diameter of oocytes during folliculogenesis in pre-pubertal gilts at marketing weight
作者: Chiou, C.M.
黃木秋
Yang, T.S.
Yeh, S.P.
Tsai, M.Z.
Cheng, S.P.
Huang, M.C.
關鍵字: porcine
follicle size
follicular fluid
oocyte
granulosa cell
in-vitro maturation
bovine oocytes
pig oocytes
developmental
competence
transcriptional activity
ovarian development
meiotic
competence
follicles
invitro
size
期刊/報告no:: Asian-Australasian Journal of Animal Sciences, Volume 17, Issue 12, Page(s) 1647-1651.
摘要: The follicles (1.8 to 7.8 mm in diameter) were recovered from the ovaries in marketed pigs and the number of granulosa cells, the diameter of oocytes obtained from different development stages of the follicles and follicular fluid levels were determined. Correlations between size measurements and cell counts as well as the diameter of antral follicles and oocytes were also investigated. The results indicated that, while expanding in size, follicle numbers decreased with a greater atretic proportion. Granulosa cells increased in numbers continuously and remained unchanged beyond the size of 200 mm(3) in non-atretic follicles, whereas a sudden drop of granulosa counts was observed in atretic follicles. Follicular fluid, on the other hand, linearly increased its volume with follicle size and differed little between those of non-atretic and atretic follicles. Diameters of oocytes in non-atretic follicles increased to its maximum when follicles expanded to 150 mm(3) and maintained its size during later follicular expansion. It is concluded that, for in vitro culture, the optimal size of porcine follicle should be between 150 to 180 mm(3) if they are collected from pre-pubertal gilts of marketing size slaughtered in an abattoir.
URI: http://hdl.handle.net/11455/45156
ISSN: 1011-2367
Appears in Collections:動物科學系

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