Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/45172
DC FieldValueLanguage
dc.contributor.authorWu, H.T.en_US
dc.contributor.author黃木秋zh_TW
dc.contributor.authorLin, C.S.en_US
dc.contributor.authorHuang, M.C.en_US
dc.date2003zh_TW
dc.date.accessioned2014-06-06T08:14:38Z-
dc.date.available2014-06-06T08:14:38Z-
dc.identifier.issn1031-3613zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/45172-
dc.description.abstractThe 5'-regulative sequence and intron 1 of the goat beta-casein gene from -4044 to +2123 by was cloned and fused with the reporter gene of green fluorescent protein (GFP) to create a plasmid termed pGB562/GFP To detect GFP expression, pGB562/GFP was transfected in vitro via liposomes into the mammary epithelial cell line NMuMG. Cells could not express GFP unless the transfected NMuMG cells lined up to create functional alveoli. These functional cells were cultured with lactogenic hormones, including insulin, dexamethasone and prolactin, and were grown on a layer of the extracellular matrix Matrigel. Green fluorescent protein expression levels in NMuMG cells were 25-, 55- and 42-fold those in the control group at 24, 48, and 72 h after pGB562/GFP transfection respectively. In addition, pGB562/GFP was transfected ex vivo by electroporation into mammary gland fragments and cells were then cultured in vitro with a supplement of lactogenic hormones. Strong GFP expression localized in fragments of the mammary gland was observed 24 h after gene transfer. The novel strategy of ex vivo gene transfer into mammary tissue using GFP as a reporter gene to detect the function of a tissue-specific promoter is efficient and convenient. The data obtained herein reveal that the 5'-regulative sequence and intron 1 of the 6.2 kb goat beta-casein gene can enhance the efficiency of transgene expression. Thus, the GB562 sequence may act as a good promoter and effectively elevate the production of exogenous protein in mammary glands.en_US
dc.language.isoen_USzh_TW
dc.relationReproduction Fertility and Developmenten_US
dc.relation.ispartofseriesReproduction Fertility and Development, Volume 15, Issue 4, Page(s) 231-239.en_US
dc.relation.urihttp://dx.doi.org/10.1071/rd01050en_US
dc.subjecthigh-level expressionen_US
dc.subjecttransgenic miceen_US
dc.subjectglucocorticoid hormonesen_US
dc.subjectalpha-lactalbuminen_US
dc.subjectmammalian-cellsen_US
dc.subjectencoding geneen_US
dc.subjectmilken_US
dc.subjectpromoteren_US
dc.subjectprolactinen_US
dc.subjectraten_US
dc.titleIn vitro and ex vivo green fluorescent protein expression in alveolar mammary epithelial cells and mammary glands driven by the distal 5 '-regulative sequence and intron 1 of the goat beta-casein geneen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1071/rd01050zh_TW
Appears in Collections:動物科學系
文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.