請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/45227
標題: Enrichment isolation, and characterization of phenol-degrading Pseudomonas resinovorans strain P-1 and Brevibacillus sp strain P-6
作者: Yang, C.F.
李季眉
Lee, C.M.
關鍵字: biodegradation
phenol
DGGE
Pseudomonas resinovorans strain p-1
Brevibacillus sp strain P-6
sphingomonas-chlorophenolica
batch reactor
degradation
biodegradation
putida
pure
期刊/報告no:: International Biodeterioration & Biodegradation, Volume 59, Issue 3, Page(s) 206-210.
摘要: The objectives of this research were to isolate pure phenol-degrading strains from enriched mixed cultures, monitoring the variations of species during the enrichment period. Two strains were isolated from the acclimated mixed culture. They were identified as Pseudomonas resinovorans strain P-1 and Brevibacillus sp. strain P-6. DGGE indicated that strain P. resinovorans appeared at the beginning, and maintained well during the enrichment period. The second strain, Brevibacillus sp., did not appear in the initial stage, but showed up after 2 weeks of enrichment. The optimum growth temperatures for P. resinovorans and Brevibacillus sp. were 31 and 39 degrees C, respectively. P. resinovorans could degrade phenol completely within 57.5 h, when the initial phenol concentration was lower than 600 mg1(-1). If the initial phenol concentration was lower than 200 mg1(-1), Brevibacillus sp. could remove phenol completely within 93.1 h. It was obvious that the phenol-degrading ability of P. resinovorans was much better than that of Brevibacillus sp. The metabolic pathway for P. resinovorans phenol degradation was assigned to the meta-cleavage activity of catechol 2,3-dioxygenase. (c) 2006 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/45227
ISSN: 0964-8305
文章連結: http://dx.doi.org/10.1016/j.ibiod.2006.09.010
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