請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/45269
標題: Enrichment, isolation, and characterization of 4-chlorophenol-degrading bacterium Rhizobium sp 4-CP-20
作者: Yang, C.F.
李季眉
Lee, C.M.
關鍵字: biodegradation
4-chlorophenol
DGGE
Rhizobium sp
4-CP-20
BIOLOG
arthrobacter-chlorophenolicus a6
gradient gel-electrophoresis
sphingomonas-chlorophenolica
waste-water
degradation
biodegradation
system
bioremediation
temperature
community
期刊/報告no:: Biodegradation, Volume 19, Issue 3, Page(s) 329-336.
摘要: The objectives of this research were to monitor the variations of species in mixed cultures during the enrichment period, isolate species and identify and characterize the pure 4-chlorophenol (4-CP) degrading strains from enriched mixed cultures. Strain Rhizobium sp. 4-CP-20 was isolated from the acclimated mixed culture. The DGGE result indicated that strain Rhizobium sp. 4-CP-20 was undetectable at the beginning but detectable after 2 weeks of enrichment. The optimum growth temperatures for Rhizobium sp. 4-CP-20 were both 36 degrees C using 350 mg l(-1) glucose or sodium acetate as the substrate. The optimum pH range for degrading 100 mg l(-1) 4-CP was between 6.89 and 8.20. Strain Rhizobium sp. 4-CP-20 could degrade 4-CP completely within 3.95 days, as the initial 4-CP concentration was 100 mg l(-1). If the initial 4-CP concentration was higher than 240 mg l(-1), the growth of bacterial cells and the activity of degrading 4-CP were both inhibited.
URI: http://hdl.handle.net/11455/45269
ISSN: 0923-9820
文章連結: http://dx.doi.org/10.1007/s10532-007-9139-1
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