請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/45374
標題: Fermentative hydrogen production and bacterial community structure in high-rate anaerobic bioreactors containing silicone-immobilized and self-flocculated sludge
作者: Wu, S.Y.
洪俊雄
Hung, C.H.
Lin, C.N.
Chen, H.W.
Lee, A.S.
Chang, J.S.
關鍵字: fermentative H-2 production
granular sludge
cell immobilization
bioreactor design
bacterial community structure
gradient gel-electrophoresis
16s ribosomal-rna
biohydrogen production
enterobacter-aerogenes
bed bioreactors
granular sludge
gas-production
sewage-sludge
reactor
cells
期刊/報告no:: Biotechnology and Bioengineering, Volume 93, Issue 5, Page(s) 934-946.
摘要: A novel continuously stirred anaerobic bioreactor (CSABR) seeded with silicone-immobilized sludge was developed for high-rate fermentative H-2 production using sucrose as the limiting substrate. The CSABR system was operated at a hydraulic retention time (HRT) of 0.5-6 h and an influent sucrose concentration of 10-40 g COD/L. With a high feeding sucrose concentration (i.e., 30-40 g COD/L) and a short HRT (0.5 h), the CSABR reactor produced H2 more efficiently with the highest volumetric rate (nu(H2)) of 15 L/h/L (i.e., 14.7 mol/d/L) and an optimal yield of ca. 3.5 mol H-2/mol sucrose. The maximum nu(H2) value obtained from this work is much higher than any other nu(H2) values ever documented. Formation of self-flocculated granular sludge occurred during operation at a short HRT. The granule formation is thought to play a pivotal role in the dramatic enhancement of H-2 production rate, because it led to more efficient biomass retention. A high biomass concentration of up to 35.4 g VSS/L was achieved even though the reactor was operated at an extremely low HRT (i.e., 0.5 h). In addition to gaining high biomass concentrations, formation of granular sludge also triggered a transition in bacterial community structure, resulting in a nearly twofold increase in the specific H-2 production rate. According to denatured-gradient-gel-electrophoresis analysis, operations at a progressively decreasing HRT resulted in a decrease in bacterial population diversity. The culture with the best H-2 production performance (at HRT = 0.5 h and sucrose concentration = 30 g COD/L) was eventually dominated by a presumably excellent H-2-producing bacterial species identified as Clostridium pasteurianum. (c) 2005 Wiley Periodicals, Inc.
URI: http://hdl.handle.net/11455/45374
ISSN: 0006-3592
文章連結: http://dx.doi.org/10.1002/bit.20800
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