請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/45380
標題: Application of Clostridium-specific PCR primers on the analysis of dark fermentation hydrogen-producing bacterial community
作者: Hung, C.H.
洪俊雄
Cheng, C.H.
Cheng, L.H.
Liang, C.M.
Lin, C.Y.
關鍵字: fermentative H(2) production
Clostridium
primer
bacterial community
structure
PCR
targeted oligonucleotide probes
16s ribosomal-rna
biohydrogen
production
microbial community
genus clostridium
sludge
acetobutylicum
populations
reactor
期刊/報告no:: International Journal of Hydrogen Energy, Volume 33, Issue 5, Page(s) 1586-1592.
摘要: Molecular method such as PCR-DGGE using well-accepted universal 16S rDNA PCR primer sets was often applied on the study of Clostridium bacterial community. However, unsatisfied results were often obtained due to the difficulty of distinguishing coexisting Clostridium and other anaerobic microorganisms. In this study, a specific PCR primer set (Chis150f-ClostIr), based on the available rRNA gene sequences from the database, targeting only the majority of Clusters I and II Clostridia was designed and tested on both the pure culture Clostridium and dark fermentation sludge. it was demonstrated that this new primer set could not only successfully distinguish the coexisted Clostridium species but also revealed the existence of some Clostridium species in the sludge which could not be detected by using the universal primer set. This method successfully provides a detailed view on the Clostridium community responsible for an effective hydrogen production in dark fermentation system. (c) 2007 Published by Elsevier Ltd. on behalf of International Association for Hydrogen Energy.
URI: http://hdl.handle.net/11455/45380
ISSN: 0360-3199
文章連結: http://dx.doi.org/10.1016/j.ijhydene.2007.09.037
顯示於類別:環境工程學系所

文件中的檔案:
沒有與此文件相關的檔案。


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。