請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/45384
標題: Microbial community structure of a starch-feeding fermentative hydrogen production reactor operated under different incubation conditions
作者: Cheng, C.H.
洪俊雄
Hung, C.H.
Lee, K.S.
Liau, P.Y.
Liang, C.M.
Yang, L.H.
Lin, P.J.
Lin, C.Y.
關鍵字: Dark fermentative hydrogen production
Starch-feeding
Microbial
community structure
PCR-DGGE
FISH
targeted oligonucleotide probes
gradient gel-electrophoresis
gram-positive bacteria
in-situ hybridization
16s ribosomal-rna
dna
g+c content
biohydrogen production
sludge
waste
temperature
期刊/報告no:: International Journal of Hydrogen Energy, Volume 33, Issue 19, Page(s) 5242-5249.
摘要: The aim of this study was to establish the particular biohydrogen-production related microbial community structure in a starch-feeding dark fermentation agitated granular sludge bed (AGSB) reactor which was operated under pH 6.0 and 5.5 as well as under different hydraulic retention times (HRTs). The bacterial community diversity and percent of their cell count of the bioreactor were ascertained using denaturing gradient get electrophoresis (DGGE) and fluorescence in situ hybridization (FISH) individually. Based on the comparison of bacterial structure and hydrogen production efficiency under different HRT, no conclusion could be made on whether the diversity of Clostridium community could directly affect the reactor performance in these two pH systems. However, bacterial cell Counts showed that the viable number of dominated Clostridium sp. changed along with the hydrogen production rate (HPR). It was believed that it could directly affect the hydrogen production efficiency. The highest HPR and hydrogen yield (HY) occurred when the reactor was operated at HRT 0.5 h, while the ratio of Clostridium sp. cell count and Bifidobacterium sp. cell count over the total Eubacteria cell count were around 40% and 40-60%, respectively. Therefore, we suggested that bacterial species which could degrade starch, such as Bifidobacterium sp. in this study, broke down starch into small molecules first and then these less complex compounds were utilized by the Clostridium species for hydrogen production. (C) 2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/45384
ISSN: 0360-3199
文章連結: http://dx.doi.org/10.1016/j.ijhydene.2008.05.017
顯示於類別:環境工程學系所

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