請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/46019
標題: CDNA cloning and characterization of S6 kinase and its effect on yolk protein gene expression in the oriental fruit fly Bactrocera dorsalis (Hendel)
作者: Suganya, R.
路光暉
Chen, S.L.
Lu, K.H.
關鍵字: S6K
yolk protein
TOR
rapamycin
RNAi
Bactrocera dorsalis
mosquito aedes-aegypti
nutritional signals
cell-size
rapamycin
drosophila
target
tor
phosphorylation
p70(s6k)
growth
期刊/報告no:: Archives of Insect Biochemistry and Physiology, Volume 78, Issue 4, Page(s) 177-189.
摘要: p70 S6 kinase (S6K), a serine/threonine protein kinase, is a downstream target of target of rapamycin (TOR) gene and an important regulator of protein synthesis responsible for cell growth and reproduction. In this study, a S6K gene, named BdS6K (GenBank Accession No. GQ203802), was isolated from the oriental fruit fly Bactrocera dorsalis (Hendel). Quantitative RT-PCR showed that BdS6K mRNA is expressed at a higher level in egg than in other developmental stages, as well as in ovary than in fat body. Downregulation of BdS6K activity by rapamycin treatment in larval stage resulted in the developmental defects of larvae, pupae, and adults, with a reduced yolk protein (YP) expression in the fat body throughout the first reproductive cycle with a substantial reduction in ovary size, and also repressed the egg development in female fruit fly. Knockdown of BdS6K gene by RNA interference in the adult significantly decreased the YP expression. These observations support the involvement of BdS6K signaling in the regulation of the YP synthesis and egg development in B. dorsalis. (C) 2011 Wiley Periodicals, Inc.
URI: http://hdl.handle.net/11455/46019
ISSN: 0739-4462
文章連結: http://dx.doi.org/10.1002/arch.20446
顯示於類別:昆蟲學系

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