Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46169
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dc.contributor.authorChang, P.F.L.en_US
dc.contributor.author張碧芳zh_TW
dc.contributor.authorJinn, T.L.en_US
dc.contributor.authorHuang, W.K.en_US
dc.contributor.authorChen, Y.S.en_US
dc.contributor.authorChang, H.M.en_US
dc.contributor.authorWang, C.W.en_US
dc.date2007zh_TW
dc.date.accessioned2014-06-06T08:18:52Z-
dc.date.available2014-06-06T08:18:52Z-
dc.identifier.issn0168-9452zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/46169-
dc.description.abstractThis is the first report of a full-length cDNA clone for a class II small heat shock protein (sHSP) isolated from rice (Oryza sativa L., cv. Tainong No. 67) etiolated seedlings heat shocked at 41 C for 2 h. The coding sequence consists of 501 bp, and the clone encodes a protein of 18.0 kDa with a predicted pI value of 5.61. The obtained full-length cDNA clone, designated Oshsp18.0-CII, is almost identical to a putative class II sHSP gene located on rice (cv. Nipponbare) chromosome one and another putative class II sHSP rice gene. Oshsp18.0-CII was induced by mechanical injury and salicylic acid treatment, which is not common in this class of sHSP genes. Only one copy of class II sHSP genes is present in the rice genome, and western blot analysis with anti-PsHSP17.7 (a class II pea sHSP) also showed only one protein of similar to 18 kDa in the 2D gel of heat-shocked rice proteins. Oshsp18.0-CII is GC-rich and contains a secondary structure in its RNA sequence. (c) 2006 Elsevier Ireland Ltd. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationPlant Scienceen_US
dc.relation.ispartofseriesPlant Science, Volume 172, Issue 1, Page(s) 64-75.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.plantsci.2006.07.017en_US
dc.subjectgene expressionen_US
dc.subjectheat shock (HS)en_US
dc.subjectheat shock protein (HSP)en_US
dc.subjectOryza sativaen_US
dc.subjectL.en_US
dc.subjectwoundingen_US
dc.subjectmultiple sequence alignmenten_US
dc.subjectlow-molecular-massen_US
dc.subjectarabidopsis-thalianaen_US
dc.subjectoryza-sativaen_US
dc.subjectdisease resistanceen_US
dc.subjectescherichia-colien_US
dc.subjectgene-expressionen_US
dc.subjectcytosolic hsp90en_US
dc.subjectabiotic stressen_US
dc.subjectin-vitroen_US
dc.titleInduction of a cDNA clone from rice encoding a class II small heat shock protein by heat stress, mechanical injury, and salicylic aciden_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.plantsci.2006.07.017zh_TW
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