Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46178
標題: Serological relationship between Melon yellow spot virus and Watermelon silver mottle virus and differential detection of the two viruses in cucurbits
作者: Chen, T.C.
葉錫東
Lu, Y.Y.
Cheng, Y.H.
Li, J.T.
Yeh, Y.C.
Kang, Y.C.
Chang, C.P.
Huang, L.H.
Peng, J.C.
Yeh, S.D.
關鍵字: papaya ringspot virus
wilt-like virus
s-rna segment
molecular
characterization
nucleotide-sequence
genus tospovirus
nonstructural
protein
intergenic region
mosaic virus-1
calla lilies
期刊/報告no:: Archives of Virology, Volume 155, Issue 7, Page(s) 1085-1095.
摘要: Melon yellow spot virus (MYSV), a tentative member of the genus Tospovirus, is considered a distinct serotype due to the lack of a serological relationship with other tospoviruses in its nucleocapsid protein (NP). Recently, a virus isolate collected from diseased watermelon in central Taiwan (MYSV-TW) was found to react with a rabbit antiserum (RAs) prepared against the NP of Watermelon silver mottle virus (WSMoV), and a monoclonal antibody (MAb) prepared against the common epitope of the NSs proteins of WSMoV-serogroup tospoviruses, but not with the WSMoV NP-specific MAb, in both enzyme-linked immunosorbent assay (ELISA) and western blotting. In this investigation, both RAs and MAb against MYSV-TW NP were produced. Results of serological tests revealed that the RAs to MYSV-TW NP reacted with the homologous antigen and the crude antigens of members of the WSMoV serogroup, including members of the formal species WSMoV and Peanut bud necrosis virus, and members of three tentative species, Watermelon bud necrosis virus, Capsicum chlorosis virus and Calla lily chlorotic spot virus. The MAb to MYSV-TW NP reacted only with the homologous antigen and the other geographic isolates of MYSV from Japan (JP) and Thailand (TH). Our results of reciprocal tests indicate that the NP and the NSs protein of MYSV are serologically related to those of WSMoV-serogroup tospoviruses. Furthermore, we show that both the MYSV NP MAb and the WSMoV NP MAb are reliable tools for identification of MYSV and WSMoV from single or mixed infection in field surveys, as verified using species-specific primers in reverse transcription-polymerase chain reaction.
URI: http://hdl.handle.net/11455/46178
ISSN: 0304-8608
文章連結: http://dx.doi.org/10.1007/s00705-010-0688-y
Appears in Collections:植物病理學系

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.