Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46198
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dc.contributor.authorWu, Z.B.en_US
dc.contributor.author詹富智zh_TW
dc.contributor.authorZheng, Y.X.en_US
dc.contributor.authorSu, C.C.en_US
dc.contributor.authorChang, C.J.en_US
dc.contributor.authorJan, F.J.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-06T08:18:54Z-
dc.date.available2014-06-06T08:18:54Z-
dc.identifier.issn0929-1873zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/46198-
dc.description.abstractA putative virus-induced disease of pear (Pyrus pyrifolia var. Hengshen) showing symptoms of reduced size of foliage and leaf distortion was observed in orchards in central Taiwan in 2004. The sap of symptomatic leaf samples reacted positively to an antiserum against Apple stem grooving virus (ASGV). Two virus cultures, designated as TS1 and TS2, were isolated from symptomatic pears. Flexuous filamentous virions of similar to 12 x 600 nm were observed in symptomatic pear leaves and purified virus preparations. Results of back inoculation of pear seedlings with TS1 revealed that ASGV was the causal agent of the disease. Sequence analyses of the cloned coat protein (CP) genes of TS1 and TS2 shared 88-92.4% nucleotide and 90.7-97.1% amino acid identities with those of other ASGV isolates available in GenBank. The polyclonal antibody generated against ASGV TS1 has been routinely used for the detection of the ASGV-infection in the imported pear scions for quarantine purpose via enzyme-linked immunosorbent assays (ELISAs). One of 1,199 samples of pear scions imported from Japan during 2005-2007 was identified as ASGV-positive and the virus was designated as AGJP-22. The CP gene amplified from this AGJP-22 shared 97.9-98.3% amino acid identities to those of the domestic isolates and they were closely related phylogenetically. To date, these data present for the first time conclusive evidence revealing that ASGV is indeed the causal agent of the pear disease displaying symptoms of reduced size of foliage and leaf distortion in Taiwan.en_US
dc.language.isoen_USzh_TW
dc.relationEuropean Journal of Plant Pathologyen_US
dc.relation.ispartofseriesEuropean Journal of Plant Pathology, Volume 128, Issue 1, Page(s) 71-79.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s10658-010-9631-zen_US
dc.subjectASGVen_US
dc.subjectCapillovirusen_US
dc.subjectRosaceaeen_US
dc.subjectPome fruiten_US
dc.subjectPear diseaseen_US
dc.subjectPhylogeneticen_US
dc.subjectanalysisen_US
dc.subjectpurificationen_US
dc.subjecttreesen_US
dc.subjectsequenceen_US
dc.subjectisolateen_US
dc.subjectgeneen_US
dc.titleIdentification and characterization of Apple stem grooving virus causing leaf distortion on pear (Pyrus pyrifolia) in Taiwanen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s10658-010-9631-zzh_TW
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