請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/46214
標題: Genetic analysis of an attenuated Papaya ringspot virus strain applied for cross-protection
作者: Chiang, C.H.
詹富智
Lee, C.Y.
Wang, C.H.
Jan, F.J.
Lin, S.S.
Chen, T.C.
Raja, J.A.J.
Yeh, S.D.
葉錫東
關鍵字: attenuated virus
cross-protection
in vitro transcription
recombinant
virus
turnip-mosaic-virus
plum-pox-virus
helper component-proteinase
vein
mottling virus
mutational analysis
plant-virus
pathogenicity
determinants
multifunctional protein
nucleotide-sequence
symptom
expression
期刊/報告no:: European Journal of Plant Pathology, Volume 118, Issue 4, Page(s) 333-348.
摘要: Papaya ringspot virus (PRSV) HA 5-1, a nitrous acid-induced mild mutant of severe strain HA, widely applied for control of PRSV by cross-protection, was used to study the genetic basis of attenuation. Using infectious clones, a series of recombinants was generated between HA 5-1 and HA and their infectivity was analyzed on the systemic host papaya and the local lesion host Chenopodium quinoa. The recombinants that contained mutations in PI and HC-Pro genes caused attenuated infection on papaya without conspicuous symptoms, similar to HA 5-1. The recombination and sequence analyses strongly implicated two amino acid changes in the C-terminal region of PI and two in HC-Pro of HA 5-1 involved in the attenuated infection on papaya. The recombinants that infected C. quinoa plants without local lesions contained the same mutations in the C-terminal region of HC-Pro for attenuated infection on papaya. We conclude that both PI and HC-Pro bear important pathogenicity determinants for the infection on the systemic host papaya and that the mutations in HC-Pro affecting pathogenicity on papaya are also responsible for the inability to induce hypersensitive reaction on C. quinoa.
URI: http://hdl.handle.net/11455/46214
ISSN: 0929-1873
文章連結: http://dx.doi.org/10.1007/s10658-007-9130-z
顯示於類別:植物病理學系

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