Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46234
標題: Development of a molecular method for rapid differentiation of watermelon lines resistant to Fusarium oxysporum f. sp niveum
作者: Lin, Y.H.
黃振文
Chen, K.S.
Liou, T.D.
Huang, J.W.
Chang, P.F.L.
張碧芳
關鍵字: Breeding programs
Citrullus lanatus
Fusarium oxysporum f. sp niveum
Fusarium wilt
Molecular detection
Molecular marker
PCR
RAPD
SCAR
wilt resistance
dna marker
identification
gene
primers
region
locus
fom-2
aflp
期刊/報告no:: Botanical Studies, Volume 50, Issue 3, Page(s) 273-280.
摘要: The vascular wilt disease of watermelon caused by Fusarium oxysporum f. sp. niveum (Fon) is one of the major factors limiting watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai) production worldwide. Breeding highly resistant lines is one of the best ways to control the disease. In order to accelerate the breeding programs, a reliable PCR (polymerase chain reaction) technique was developed to rapidly differentiate the disease-resistant watermelons. The G05-SCAR (sequence characterized amplified region) primer set GsF5/GsR5, derived from the OP-G05 random primer-amplified fragment sequence, produced an 898 bp DNA fragment which was specific to watermelon lines resistant or tolerant to Fon. With optimized PCR assay, the Molecular method could easily differentiate Fon-resistant and -susceptible hybrid watermelon populations.
URI: http://hdl.handle.net/11455/46234
ISSN: 1817-406X
Appears in Collections:植物病理學系

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