Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46235
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dc.contributor.authorLin, Y.H.en_US
dc.contributor.author黃振文zh_TW
dc.contributor.authorChang, J.Y.en_US
dc.contributor.authorLiu, E.T.en_US
dc.contributor.authorChao, C.P.en_US
dc.contributor.authorHuang, J.W.en_US
dc.contributor.authorChang, P.F.L.en_US
dc.contributor.author張碧芳zh_TW
dc.date2009zh_TW
dc.date.accessioned2014-06-06T08:18:57Z-
dc.date.available2014-06-06T08:18:57Z-
dc.identifier.issn0929-1873zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/46235-
dc.description.abstractFusarium oxysporum f. sp. cubense is the causal agent of Panama disease of banana. A rapid and reliable diagnosis is the foundation of integrated disease management practices in commodity crops. For this diagnostic purpose, we have developed a reliable molecular method to detect Foc race 4 isolates in Taiwan. By PCR amplification, the primer set Foc1/Foc-2 derived from the sequence of a random primer OP-A02 amplified fragment produced a 242 bp size DNA fragment which was specific to Foc race 4. With the optimized PCR parameters, the molecular method was sensitive and could detect small quantities of Foc DNA as low as 10 pg in 50 to 2,000 ng host genomic DNA with high efficiency. We also demonstrated that by using our PCR assay with Foc-1/Foc-2 primer set, Foc race 4 could be easily distinguished from other Foc races 1 and 2, and separated other formae speciales of F. oxysporum.en_US
dc.language.isoen_USzh_TW
dc.relationEuropean Journal of Plant Pathologyen_US
dc.relation.ispartofseriesEuropean Journal of Plant Pathology, Volume 123, Issue 3, Page(s) 353-365.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s10658-008-9372-4en_US
dc.subjectMolecular detectionen_US
dc.subjectPanama diseaseen_US
dc.subjectPCRen_US
dc.subjectRAPDen_US
dc.subjectReliable diagnosisen_US
dc.subjectvegetative compatibility groupsen_US
dc.subjectpcr assaysen_US
dc.subjectcavendish bananaen_US
dc.subjectpanama-diseaseen_US
dc.subjectwilten_US
dc.subjectculmorumen_US
dc.subjectgraminearumen_US
dc.subjectresistanceen_US
dc.subjectdiversityen_US
dc.subjectavenaceumen_US
dc.titleDevelopment of a molecular marker for specific detection of Fusarium oxysporum f. sp cubense race 4en_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s10658-008-9372-4zh_TW
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