Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50218
標題: Preparation and properties of gold nanoparticle-electrodeposited titanium substrates with Arg-Gly-Asp-Cys peptides
作者: Weng, H.A.
吳靖宙
Wu, C.C.
Chen, C.G.
Ho, C.C.
Ding, S.J.
關鍵字: surface modification
covalent attachment
bone-formation
cell-adhesion
rgd peptide
binding
particles
implants
ti-6al-4v
molecules
期刊/報告no:: Journal of Materials Science-Materials in Medicine, Volume 21, Issue 5, Page(s) 1511-1519.
摘要: Titanium metal has good biocompatibility, superior mechanical properties and excellent corrosion resistance. Like most metals, however, it exhibits poor bioactive properties and fails to bond to bone tissue. To improve its bioactivity, bioactive molecules, such as peptides, can be grafted onto titanium surfaces. In order to do this, the first step may be to establish a stable and compatible linking layer on the titanium surface. In this study, we used electrochemical methods to deposit gold (Au) nanoparticles onto titanium substrates, to which we then grafted arginine-glycine-asparagine-cysteine (RGDC) peptides by thiolate covalent coupling. Properties of electrodeposited Au nanoparticles were evaluated using a variety of techniques, including microstructural, chemical and electrochemical measurements. The biological responses of the RGDC-grafted Ti substrates were evaluated using MG3 human osteoblast-like cells. The results of thin-film X-ray diffraction (TFXRD) and scanning electron microscopy (SEM) indicated the polycrystalline orientation of Au nanoparticles deposited on the titanium surfaces with high density and controllable particle size. The RGDC peptide could be covalently bonded to Au-deposited Ti substrates via Au-thiolate species, as expected. Cell morphology showed that, on RGDC-immobilized titanium with Au particles, MG63 cells attached and spread more rapidly than on Ti substrates either without peptide or with direct loading of the peptide. Immunostaining for focal adhesion kinase (FAK) demonstrated that RGDC enhanced cell attachment. The present method for the formation of Au nanoparticles may serve as an alternative route for bioactive molecule immobilization on Ti implants.
URI: http://hdl.handle.net/11455/50218
ISSN: 0957-4530
文章連結: http://dx.doi.org/10.1007/s10856-010-4026-4
Appears in Collections:生物產業機電工程學系

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