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標題: Development of a disperse dye immunochromatographic test for the detection of antibodies against infectious bursal disease virus
作者: Wang, S.J.
Chang, W.F.
Wang, M.Y.
Hsiung, K.P.
Liu, Y.C.
關鍵字: Disperse dyes
Immunochromatographic test
Enzyme-linked immunosorbent
Infectious bursal disease virus
Point of need testing (PONT)
protein vp2
strip test
期刊/報告no:: Veterinary Immunology and Immunopathology, Volume 125, Issue 3-4, Page(s) 284-290.
摘要: For investigating the feasibility of using disperse dyes as an immunoassay chromogenic marker, a disperse dye, DADISPERSE NAVY BLUE SP, was selected in analyzing antibody against infectious bursal disease virus (anti-IBDV). With the color intensity revealed in the disperse dye immunochromatographic test (DICT) strip as the objective function, the optimal dyeing conditions were found as follows: dye concentration absorbance (at lambda(max) = 587 nm) = 3, pH 7, 50 degrees C, for 10 min. Under these conditions, the resultant dyed-antibody (rabbit anti-chicken) can produce an optimal color intensity reading of 55,054 on the strip. For performing qualitative immunoassay, chicken sera samples taken from different farms were used for the anti-IBDV titre assessment. The results of DICT strips showed very high sensitivity and specificity as compared to that analyzed by FlockChek enzyme linked immunosorbent assay (F-ELISA) kits. For quantitative immunoassay, it was found that the color intensity measured with DICT was linearly correlated to that of F-ELISA titre (r(2) = 0.9687). Therefore, DICT was further applied to the detection of chicken anti-IBDV sera under vaccination in the farms. The average titres of the sampling groups exhibited a strong agreement to that of F-ELISA. Accordingly, the DICT method developed in this study, shown to be reliable, cheap and simple in both qualitative and quantitative immunoassays, is particularly suitable for point-of-need testing (PONT) in agricultural applications. (c) 2008 Elsevier B.V. All rights reserved.
ISSN: 0165-2427
Appears in Collections:生物科技學研究所



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