Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50289
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dc.contributor.authorHsu, S.W.en_US
dc.contributor.author王國祥zh_TW
dc.contributor.authorCheng, C.L.en_US
dc.contributor.authorTzen, T.C.J.en_US
dc.contributor.authorWang, C.S.en_US
dc.contributor.author曾志正zh_TW
dc.date2010zh_TW
dc.date.accessioned2014-06-06T08:49:14Z-
dc.date.available2014-06-06T08:49:14Z-
dc.identifier.issn0032-0781zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/50289-
dc.description.abstractHere, we report unique desiccation-associated ABA signaling transduction through which the Rop (Rho GTPase of plants) gene is regulated during the stage of pollen maturation. A gene encoding Rho GTPase was identified in lily (Lilium longiflorum Thunb.) pollen. Phylogenetic tree analysis of lily LLP-Rop1 revealed that the protein shares greatest similarity with Group 4 Rops. The LLP-Rop1 gene was spatially and temporally regulated in lily plants during anther development. Accumulation of the LLP-Rop1 transcript decreased its level of accumulation while LLP-12-2, a Rop-interactive CRIB motif-containing (RIC) transcript increased either by premature drying of developing anther/pollen or by the exogenous application of various concentrations of abscisic acid (ABA) during pollen maturation and tube growth. Application of norflurazon, an ABA biosynthesis inhibitor, also resulted in the downregulation of the LLP-Rop1 gene while LLP-12-2 was upregulated by ABA. Furthermore, an increase in ABA in the maturing pollen correlated with desiccation that occurred in the anther prior to anthesis. LLP-Rop1 overexpression inhibited tube elongation, and caused tube expansion and the formation of a ballooned tip. CFP-LLP-Rop1 was localized to the cytoplasm having a greater intensity along the tube plasma membrane. Fluorescence resonance energy transfer analysis of lily pollen tubes coexpressing CFP-LLP-Rop1 and YFP-LLP-12-2 demonstrated that LLP-12-2 is a target RIC protein of active LLP-Rop1, but the interaction between LLP-Rop1 and LLP-12-2 proteins is probably irrelevant of dehydration in the dried pollen.en_US
dc.language.isoen_USzh_TW
dc.relationPlant and Cell Physiologyen_US
dc.relation.ispartofseriesPlant and Cell Physiology, Volume 51, Issue 7, Page(s) 1197-1209.en_US
dc.relation.urihttp://dx.doi.org/10.1093/pcp/pcq076en_US
dc.subjectDesiccationen_US
dc.subjectLily (Lilium longiflorum)en_US
dc.subjectPollen maturationen_US
dc.subjectRIC proteinen_US
dc.subjectRop GTPaseen_US
dc.subjectTube growthen_US
dc.subjectlilium-longiflorumen_US
dc.subjecttube growthen_US
dc.subjectnegative regulatoren_US
dc.subjectarabidopsisen_US
dc.subjectstressen_US
dc.subjectantheren_US
dc.subjectsignalen_US
dc.subjectrevealsen_US
dc.subjectpathwayen_US
dc.subjectosracben_US
dc.titleRop GTPase and Its Target Cdc42/Rac-Interactive-Binding Motif-Containing Protein Genes Respond to Desiccation during Pollen Maturationen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1093/pcp/pcq076zh_TW
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