Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50298
標題: Characterization of the AdoMet-dependent guanylyltransferase activity that is associated with the N terminus of bamboo mosaic virus replicase
作者: Li, Y.I.
徐堯煇
Chen, Y.J.
Hsu, Y.H.
Meng, M.H.
孟孟孝
關鍵字: rna-capping enzyme
escherichia-coli
methyltransferase activity
saccharomyces-cerevisiae
protein
expression
polymerase
nsp1
identification
helicases
期刊/報告no:: Journal of Virology, Volume 75, Issue 2, Page(s) 782-788.
摘要: Bamboo mosaic virus (BaMV), a member of the potexvirus group, infects primarily members of the Bambusoideae. Open reading frame 1 (ORF1) of BaMV encodes a 155-kDa polypeptide that has long been postulated to be a replicase involved in the replication and formation of the cap structure at the 5' end of the viral genome. To identify and characterize the enzymatic activities associated with the N-terminal domain of the BaMV ORF1 protein, the intact replicase and two C-terminally truncated proteins were expressed in Saccharomyces cerevisiae. All three versions of BaMV ORF1 proteins could be radiolabeled by [alpha-P-32]GTP, which is a characteristic of guanylyltransferase activity. The presence of S-adenosylmethionine (AdoMet) was essential for this enzymatic activity. Thin-layer chromatography analysis suggests that the radiolabeled moiety linked to the N-terminal domain of the BaMV ORF1 protein is m(7) GMP. The N-terminal domain also exhibited methyltransferase activity that catalyzes the transfer of the [H-3]methyl group from AdoMet to GTP or guanylylimidodiphosphate. Therefore, during cap structure formation in BaMV methylation of GTP may occur prior to transguanylation as for alphaviruses and brome mosaic virus. This study establishes the association of RNA capping activity with the N-terminal domain of the replicase of potexviruses and further supports the idea that the reaction sequence of RNA. capping is conserved throughout the alphavirus-like superfamily of RNA. viruses.
URI: http://hdl.handle.net/11455/50298
ISSN: 0022-538X
文章連結: http://dx.doi.org/10.1128/jvi.75.2.782-788.2001
Appears in Collections:生物科技學研究所

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