Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50300
標題: Functional analysis of the cloverleaf-like structure in the 3 ' untranslated region of bamboo mosaic potexvirus RNA revealed dual roles in viral RNA replication and long distance movement
作者: Chen, I.H.
蔡慶修
Meng, M.H.
Hsu, Y.H.
Tsai, C.H.
徐堯煇
孟孟孝
關鍵字: bamboo mosaic potexvirus
RNA replication
helicase-like domain
long
distance movement
helicase-like domain
virus coat protein
cell-to-cell
nucleotide-sequence
genomic rna
h-protein
identification
polymerases
valylation
potyvirus
期刊/報告no:: Virology, Volume 315, Issue 2, Page(s) 415-424.
摘要: The 3' untranslated region (UTR) of bamboo mosaic potexvirus (BaMV) RNA was identified to fold into a tertiary structure comprising a cloverleaf-like structure designated ABC domain followed by a major stem-loop D, which in turn is followed by a pseudoknot E and a poly(A) tail. The coat protein accumulation level of the mutant, BaMV40A/DeltaABC, lacking ABC domain was just 15% that of wild-type when inoculated into protoplasts of Nicotiana benthamiana. This suggested that ABC domain might play an important role in BaMV RNA replication. To define the precise role of each of the three stem-loops of ABC domain in RNA replication, three mutants BaMV40A/DeltaA, -/DeltaB, and -/DeltaC each lacking stem-loop A, B, and C, respectively, were created. Our results showed that accumulation of viral products of mutants BaMV40A/DeltaB and -/DeltaC were not as efficient as wild-type. On the contrary, level of accumulation of viral products of BaMV/DeltaA was similar to that of wild-type in protoplasts and inoculated leaves. Interestingly, the accumulation of viral products was not as efficient as that of wild-type in systemic leaves, implying that stem-loop A is dispensable for replication, but signifies a role in systemic accumulation. Using UV cross-linking and competition experiments, it was demonstrated that the E. coli expressed helicase domain of BaMV ORF1 can preferentially interact with the ABC domain. (C) 2003 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/50300
ISSN: 0042-6822
文章連結: http://dx.doi.org/10.1016/s0042-6822(03)00560-9
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