請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/50314
標題: Characterization of a desiccation-related protein in lily pollen during development and stress
作者: Wang, C.S.
王國祥
Liau, Y.E.
Huang, J.C.
Wu, T.D.
Su, C.C.
Lin, C.H.
關鍵字: amino acid sequencing
desiccation
Lilium longiflorum
pollen protein
stress-induced
heat-stable proteins
gene-expression
abscisic-acid
lilium-longiflorum
water-deficit
maturation proteins
cell-suspension
osmotic-stress
tolerance
electrophoresis
期刊/報告no:: Plant and Cell Physiology, Volume 39, Issue 12, Page(s) 1307-1314.
摘要: This work characterizes a lily (Lilium longiflorum Thunb. cv, Snow Queen) anther (LLA) protein associated with desiccation. Peptide mapping analysis revealed that the abundant LLA-23 doublet contained similar polypeptides, having an isoelectric point of 6.1. Immunoblots of pollen protein from developing anther/pollen confirmed that the LLA-23 protein accumulated only at the later stage of pollen maturation and that the levels remained steady in mature and vital pollen. The accumulation of LLA-23 proteins was correlated with desiccation that naturally occurred in pollen. Subcellular fractionation of pollen proteins revealed that the protein was located in the cytoplasmic fraction. Premature drying of developing pollen confirmed that the concomitant accumulation of LLA-23 was associated with desiccation. Peptide sequence analysis demonstrates similarities between the lily LLA-23 and a family of water-deficit/ripening-induced proteins including LP3 of pine, DS2 of potato, and Asr of tomato and pummelo, In addition, the concomitant accumulation of LLA-23 can be experimentally manipulated by methyl jasmonate (MeJA) and salicylic acid (SA as well as by mannitol and methyl viologen. The LLA-23 represents a novel member of the water-deficit/ripenine-induced proteins.
URI: http://hdl.handle.net/11455/50314
ISSN: 0032-0781
顯示於類別:生物科技學研究所

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