Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50314
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dc.contributor.authorWang, C.S.en_US
dc.contributor.author王國祥zh_TW
dc.contributor.authorLiau, Y.E.en_US
dc.contributor.authorHuang, J.C.en_US
dc.contributor.authorWu, T.D.en_US
dc.contributor.authorSu, C.C.en_US
dc.contributor.authorLin, C.H.en_US
dc.date1998zh_TW
dc.date.accessioned2014-06-06T08:49:18Z-
dc.date.available2014-06-06T08:49:18Z-
dc.identifier.issn0032-0781zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/50314-
dc.description.abstractThis work characterizes a lily (Lilium longiflorum Thunb. cv, Snow Queen) anther (LLA) protein associated with desiccation. Peptide mapping analysis revealed that the abundant LLA-23 doublet contained similar polypeptides, having an isoelectric point of 6.1. Immunoblots of pollen protein from developing anther/pollen confirmed that the LLA-23 protein accumulated only at the later stage of pollen maturation and that the levels remained steady in mature and vital pollen. The accumulation of LLA-23 proteins was correlated with desiccation that naturally occurred in pollen. Subcellular fractionation of pollen proteins revealed that the protein was located in the cytoplasmic fraction. Premature drying of developing pollen confirmed that the concomitant accumulation of LLA-23 was associated with desiccation. Peptide sequence analysis demonstrates similarities between the lily LLA-23 and a family of water-deficit/ripening-induced proteins including LP3 of pine, DS2 of potato, and Asr of tomato and pummelo, In addition, the concomitant accumulation of LLA-23 can be experimentally manipulated by methyl jasmonate (MeJA) and salicylic acid (SA as well as by mannitol and methyl viologen. The LLA-23 represents a novel member of the water-deficit/ripenine-induced proteins.en_US
dc.language.isoen_USzh_TW
dc.relationPlant and Cell Physiologyen_US
dc.relation.ispartofseriesPlant and Cell Physiology, Volume 39, Issue 12, Page(s) 1307-1314.en_US
dc.subjectamino acid sequencingen_US
dc.subjectdesiccationen_US
dc.subjectLilium longiflorumen_US
dc.subjectpollen proteinen_US
dc.subjectstress-induceden_US
dc.subjectheat-stable proteinsen_US
dc.subjectgene-expressionen_US
dc.subjectabscisic-aciden_US
dc.subjectlilium-longiflorumen_US
dc.subjectwater-deficiten_US
dc.subjectmaturation proteinsen_US
dc.subjectcell-suspensionen_US
dc.subjectosmotic-stressen_US
dc.subjecttoleranceen_US
dc.subjectelectrophoresisen_US
dc.titleCharacterization of a desiccation-related protein in lily pollen during development and stressen_US
dc.typeJournal Articlezh_TW
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