Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50322
標題: Biochemical characterization of a pollen-specific cDNA encoding polygalacturonase in Lilium longiflorum
作者: Chiang, J.Y.
王國祥
Shu, S.W.
Ko, C.W.
Wang, C.S.
關鍵字: cDNA
Lilium longiflorum
pollen-specific
polygalacturonase
pectin methylesterase
male gametophyte
gene-expression
messenger-rnas
major allergen
tube growth
protein
identification
stress
fruit
期刊/報告no:: Plant Science, Volume 170, Issue 3, Page(s) 433-440.
摘要: A pollen-specific transcript, designated LLP-A1.1, in lily (Lilium longiflorian Thunb. cv. Snow Queen) plants was isolated and characterized. The full-length cDNA encodes polygalacturonase (PG) having a sequence of 413 amino acids, a calculated molecular mass of 44 kDa, and a calculated pI of 8.1. Assessment of the hydropathy shows that the protein contains a hydrophobic segment at the N-terminus, indicating the presence of a putative signal peptide. The LLP-PG sequence displays significant resemblance to known pollen PG from various species and a group of major allergens including Ph1 p 13 of timothy grass and Pla a 2 of London planetree. Antiserum was raised against the overexpressed rLLP-PG protein in E. coli. Affinity-purified antibodies were prepared from antiserum to investigate the specificity and distribution of the protein during development. Immunoblot analyses of total protein from floral and vegetative organs confirmed that LLP-PG accumulated to detectable levels only in a discrete stage of anther development. It is a heterogeneous glycoprotein, consisting of four major and two minor polypeptides. Premature drying of developing anthers indicated that the lily LlpPG gene expression was not induced by desiccation that naturally occurs in pollen prior to anthesis. (c) 2005 Elsevier Ireland Ltd. All fights reserved.
URI: http://hdl.handle.net/11455/50322
ISSN: 0168-9452
文章連結: http://dx.doi.org/10.1016/j.plantsci.2005.09.010
Appears in Collections:生物科技學研究所

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