Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50322
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dc.contributor.authorChiang, J.Y.en_US
dc.contributor.author王國祥zh_TW
dc.contributor.authorShu, S.W.en_US
dc.contributor.authorKo, C.W.en_US
dc.contributor.authorWang, C.S.en_US
dc.date2006zh_TW
dc.date.accessioned2014-06-06T08:49:19Z-
dc.date.available2014-06-06T08:49:19Z-
dc.identifier.issn0168-9452zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/50322-
dc.description.abstractA pollen-specific transcript, designated LLP-A1.1, in lily (Lilium longiflorian Thunb. cv. Snow Queen) plants was isolated and characterized. The full-length cDNA encodes polygalacturonase (PG) having a sequence of 413 amino acids, a calculated molecular mass of 44 kDa, and a calculated pI of 8.1. Assessment of the hydropathy shows that the protein contains a hydrophobic segment at the N-terminus, indicating the presence of a putative signal peptide. The LLP-PG sequence displays significant resemblance to known pollen PG from various species and a group of major allergens including Ph1 p 13 of timothy grass and Pla a 2 of London planetree. Antiserum was raised against the overexpressed rLLP-PG protein in E. coli. Affinity-purified antibodies were prepared from antiserum to investigate the specificity and distribution of the protein during development. Immunoblot analyses of total protein from floral and vegetative organs confirmed that LLP-PG accumulated to detectable levels only in a discrete stage of anther development. It is a heterogeneous glycoprotein, consisting of four major and two minor polypeptides. Premature drying of developing anthers indicated that the lily LlpPG gene expression was not induced by desiccation that naturally occurs in pollen prior to anthesis. (c) 2005 Elsevier Ireland Ltd. All fights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationPlant Scienceen_US
dc.relation.ispartofseriesPlant Science, Volume 170, Issue 3, Page(s) 433-440.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.plantsci.2005.09.010en_US
dc.subjectcDNAen_US
dc.subjectLilium longiflorumen_US
dc.subjectpollen-specificen_US
dc.subjectpolygalacturonaseen_US
dc.subjectpectin methylesteraseen_US
dc.subjectmale gametophyteen_US
dc.subjectgene-expressionen_US
dc.subjectmessenger-rnasen_US
dc.subjectmajor allergenen_US
dc.subjecttube growthen_US
dc.subjectproteinen_US
dc.subjectidentificationen_US
dc.subjectstressen_US
dc.subjectfruiten_US
dc.titleBiochemical characterization of a pollen-specific cDNA encoding polygalacturonase in Lilium longiflorumen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.plantsci.2005.09.010zh_TW
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