請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/50324
標題: The LLA23 protein translocates into nuclei shortly before desiccation in developing pollen grains and regulates gene expression in Arabidopsis
作者: Yang, C.Y.
王國祥
Wu, C.H.
Jauh, G.
Huang, J.C.
Lin, C.C.
Wang, C.S.
關鍵字: ASR gene
Desiccation
Gene expression
L
longiflorum
Microarray
Pollen
tomato lycopersicon-esculentum
lilium-longiflorum
water-deficit
asr
protein
lily pollen
dna-binding
stress
tolerance
identification
localization
期刊/報告no:: Protoplasma, Volume 233, Issue 3-4, Page(s) 241-254.
摘要: We have isolated the LLA23 gene in the pollen of Lilium longiflorum. The LLA23 gene encodes an ASR (named after abscisic acid, stress and ripening) protein that has a nuclear localization sequence at the C terminus. The gene is interrupted by one single intron and possesses a long 5'-untranslated region. Southern blots of lily genomic DNA indicated that LLA23 is a member of a small gene family. We examined the link between LLA23 location and the desiccation that naturally occurs in developing anthers using immunogold labeling. When pollen reached maturity, a significant increase in LLA23 labeling was observed in the nuclei of both vegetative and generative cells from 10- to 12-cm buds and thereafter. This clearly demonstrates that a marked increase in LLA23 translocation from the cytoplasm to both nuclei of pollen grains occurs in 12-cm buds, a stage shortly before the commencement of desiccation during anther development. In addition, microarray analysis showed that 410 (206 up-regulated and 204 down-regulated) genes have altered expression in LLA23-overexpressing plants. Quantitative PCR analysis confirmed the changes in mRNA levels observed in our microarray analysis. This genome-wide overview of gene expression supports the theory that LLA23 acts as a regulator.
URI: http://hdl.handle.net/11455/50324
ISSN: 0033-183X
文章連結: http://dx.doi.org/10.1007/s00709-008-0016-5
顯示於類別:生物科技學研究所

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