Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50362
標題: Biological functions of the cytoplasmic TGBp1 inclusions of bamboo mosaic potexvirus
作者: Hsu, H.T.
徐堯煇
Hsu, Y.H.
Bi, I.P.
Lin, N.S.
Chang, B.Y.
關鍵字: triple gene-block
cell-to-cell
virus movement protein
stranded
nucleic-acids
long-distance movement
size-exclusion limit
subcellular-localization
nucleotide-sequence
binding properties
tubular structures
期刊/報告no:: Archives of Virology, Volume 149, Issue 5, Page(s) 1027-1035.
摘要: The TGBp1 protein encoded by the first open reading frame of the triple-gene-block (TGB) of bamboo mosaic potexvirus (BaMV) plays important roles in virus movement; one of them is to shape the viral RNA before its being transported from cell to cell. However, TGBp1 mainly forms cytoplasmic inclusions which are devoid of the RNA-binding activity. With the purified TGBp1 inclusions of BaMV, we analyzed the possibility of dissociation of functional TGBp1 from the inclusions. Our data showed that the cytoplasmic inclusions were able to dissociate continuously functional TGBp1, which possessed ATP-binding, ATPase, and RNA-binding activities. Moreover, the dissociation of TGBp1 was significantly enhanced by the presence of viral RNA and capsid protein (CP), consistent with the finding that TGBp1 are able to form ribonucleoprotein complex with viral RNA and CP. Taken together, these results support the idea that the cytoplasmic TGBp1 inclusions are active pools of TGBp1, upon which viral RNA can be shaped into a transferable form.
URI: http://hdl.handle.net/11455/50362
ISSN: 0304-8608
文章連結: http://dx.doi.org/10.1007/s00705-003-0254-y
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