請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/50369
標題: Subcellular localization and expression of bamboo mosaic virus satellite RNA-encoded protein
作者: Palani, P.V.
徐堯煇
Chiu, M.
Chen, W.
Wang, C.C.
Lin, C.C.
Hsu, C.C.
Cheng, C.P.
Chen, C.M.
Hsu, Y.H.
Lin, N.S.
關鍵字: black ring virus
triple-gene-block
to-cell movement
nucleotide-sequence
capsid protein
uneven distribution
chenopodium-quinoa
plant-viruses
genomic rna
potexvirus
期刊/報告no:: Journal of General Virology, Volume 90, Page(s) 507-518.
摘要: The satellite RNA of bamboo mosaic virus (satBaMV) has a single open reading frame encoding a non-structural protein, P20, which facilitates long-distance movement of satBaMV in BaMV and satBaMV co-infected plants. Immunohistochemistry and immunoelectron microscopy revealed that the P20 protein accumulated in the cytoplasm and nuclei in co-infected cells. P20 and the helper virus coat protein (CP) were highly similar in their subcellular localization, except that aggregates of BaMV virions were not labelled with anti-P20 serum. The BaMV CP protein was fairly abundant in mesophyll cells, whilst P20 was more frequently detected in mesophyll cells and vascular tissues. The expression kinetics of the P20 protein was similar to but slightly earlier than that of CP in co-infected Bambusa oldhamii protoplasts and Nicotiana benthamiana leaves. However, satBaMV-encocled protein levels declined rapidly in the late phase of co-infection. During co-infection, in addition to the intact P20, a low-molecular-mass polypeptide of 16 kDa was identified as a P20 C-terminally truncated product; the possible method of generation of the truncated protein is discussed.
URI: http://hdl.handle.net/11455/50369
ISSN: 0022-1317
文章連結: http://dx.doi.org/10.1099/vir.0.004994-0
顯示於類別:生物科技學研究所

文件中的檔案:
沒有與此文件相關的檔案。


在 DSpace 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。