Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50372
DC FieldValueLanguage
dc.contributor.authorTsai, M.S.en_US
dc.contributor.author徐堯煇zh_TW
dc.contributor.authorHsu, Y.H.en_US
dc.contributor.authorLin, N.S.en_US
dc.date1999zh_TW
dc.date.accessioned2014-06-06T08:49:23Z-
dc.date.available2014-06-06T08:49:23Z-
dc.identifier.issn0022-538Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/50372-
dc.description.abstractA satellite RNA of 836 nucleotides [excluding the poly(A) tail] depends on the bamboo mosaic potexvirus (BaMV) for its replication and encapsidation. The BaMV satellite RNA (satBaMV) contains a single open reading frame encoding a 20-kDa nonstructural protein (P20). The P20 protein with eight histidine residues at the C terminus was overexpressed in Escherichia coli. Experiments of gel retardation, UV cross-linking, and Northwestern hybridization demonstrated that purified P20 was a nucleic-acid-binding protein. The binding of P20 to nucleic acids was strong and highly cooperative. P20 preferred binding to satBaMV- or BaMV-related sequences rather than to nonrelated sequences, By deletion analysis, the P20 binding sites were mainly located at the 5' and 3' untranslated regions of satBaMV RNA, and the RNA-protein interactions could compete with the poly(G) and, less efficiently, with the poly(U) homopolymers, The N-terminal arginine-rich motif of P20 was the RNA binding domain, as shown by in-frame deletion analysis. This is the first report that a plant virus satellite RNA-encoded nonstructural protein preferentially binds with nucleic acids.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Virologyen_US
dc.relation.ispartofseriesJournal of Virology, Volume 73, Issue 4, Page(s) 3032-3039.en_US
dc.subjecthepatitis-delta-virusen_US
dc.subjectnucleic-aciden_US
dc.subjectmovement proteinen_US
dc.subjectcoat proteinen_US
dc.subjectnucleotide-sequenceen_US
dc.subjectcooperative bindingen_US
dc.subjectencoded proteinen_US
dc.subjectreplicationen_US
dc.subjectpeptidesen_US
dc.subjectantigenen_US
dc.titleBamboo mosaic potexvirus satellite RNA (satBaMV RNA)-encoded P20 protein preferentially binds to satBaMV RNAen_US
dc.typeJournal Articlezh_TW
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