Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50397
標題: Gene families encoding 11S globulin and 2S albumin isoforms of jelly fig (Ficus awkeotsang) achenes
作者: 曾志正
Chua, A.C.N.
Hsiao, E.S.L.
Yang, Y.C.
Lin, L.J.
Chou, W.M.
Tzen, J.T.C.
關鍵字: achene
expressed sequence tag
Ficus awkeotsang
matrix-assisted laser
desorption/ionization mass spectrometry
seed storage proteins
acidic pectin methylesterase
seed storage proteins
identification
sesame
cloning
expression
allergen
期刊/報告no:: Bioscience Biotechnology and Biochemistry, Volume 72, Issue 2, Page(s) 506-513.
摘要: Seed storage proteins of plants commonly comprise several groups of multiple isoforms encoded by gene families. From about 300 expressed sequence tag (EST) clones in maturing jelly fig (Ficus awkeotsang Makino) achenes, gene families encoding precursor polypeptides of two storage protein classes, including six Its globulin isoforms and two 2S albumin isoforms, were identified. Complete sequences encoding the precursor polypeptides of these eight storage proteins were obtained by sequencing the pertinent EST clones that contained full-length cDNA fragments. Matrix-assisted laser desorption/ionization mass spectrometry analysis confirmed the presence of these storage protein isoforms in the extract of jelly fig achenes resolved in SDS-PAGE. The amino acid compositions of the deduced storage proteins indicated that achene proteins in jelly fig are nutritive, for both isoforms of 2S albumin are sulfur-rich, and one of them is also rich in tryptophan.
URI: http://hdl.handle.net/11455/50397
ISSN: 0916-8451
文章連結: http://dx.doi.org/10.1271/bbb.70588
Appears in Collections:生物科技學研究所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.