Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50401
標題: Size and stability of reconstituted sesame oil bodies
作者: 曾志正
Peng, C.C.
Lin, I.P.
Lin, C.K.
Tzen, J.T.C.
關鍵字: in-vitro evaluation
blue pigments
proteins
oleosin
cytotoxicity
biogenesis
geniposide
caleosin
genipin
surface
期刊/報告no:: Biotechnology Progress, Volume 19, Issue 5, Page(s) 1623-1626.
摘要: Oil bodies of sesame seeds comprise a triacylglycerol matrix, which is surrounded by a monolayer of phospholipids embedded with unique proteins, mainly structural proteins termed oleosins. Artificial oil bodies were successfully reconstituted with various compositions of triacylglycerols, phospholipids, and oil-body proteins. The sizes of reconstituted oil bodies displayed a normal distribution with an average size proportional to the ratio of triacylglycerols to oil-body proteins. Both thermostability and structural stability of reconstituted oil bodies decreased as their sizes increased, and vice versa. Proteinase K digestion indicated that oleosins anchored both native and reconstituted oil bodies via their central hydrophobic domains. The stability of reconstituted oil bodies, as well as the purified ones from sesame seeds, could be substantially enhanced after their surface proteins were cross-linked by glutaraldehyde or genipin.
URI: http://hdl.handle.net/11455/50401
ISSN: 8756-7938
文章連結: http://dx.doi.org/10.1021/bp034129z
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