Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50404
標題: Ratiometric imaging of gastrodermal lipid bodies in coral-dinoflagellate endosymbiosis
作者: 曾志正
Luo, Y.J.
Wang, L.H.
Chen, W.N.U.
Peng, S.E.
Tzen, J.T.C.
Hsiao, Y.Y.
Huang, H.J.
Fang, L.S.
Chen, C.S.
關鍵字: Coral bleaching
Nile red
Membrane trafficking
Lipid droplets
Symbiodinium
freshly isolated zooxanthellae
nile-red
symbiotic algae
cell-death
droplets
fluorescence
microscopy
probe
triacylglycerol
phytoplankton
期刊/報告no:: Coral Reefs, Volume 28, Issue 1, Page(s) 289-301.
摘要: Cnidaria-dinoflagellate endosymbiosis is the phenomenon of autotrophic symbionts living inside the gastrodermal cells of their animal hosts. The molecular mechanism that regulates this association remains unclear. Using quantitative microscopy, we now provide evidence that the dynamic lipid changes in gastrodermal "lipid bodies" (LBs) reflect the symbiotic status of the host cell and its symbiont in the hermatypic coral Euphyllia glabrescens. By dual-emission ratiometric imaging with a solvatochromic fluorescent probe, Nile red (9-diethylamino-5H-benzo[alpha]phenoxazine-5-one), we showed that the in situ distribution of polar versus neutral lipids in LBs in living gastrodermal cells and symbionts can be analyzed. The ratio of Nile red fluorescence at red (R) versus green (G) wavelength region (i.e., R/G ratio) correlated with the relative molar ratio of polar (P) versus neutral (NP) lipids (i.e., P/NP ratio). The R/G ratio in host LBs increased after bleaching, indicating a decrease in neutral lipid accumulation in gastrodermal cells. On the other hand, neutral lipid accumulation inside the symbiont LBs resulted in gradual decreases of the R/G ratio as a result of bleaching. In comparison with the bleaching event, there was no relative lipid concentration change in host LBs under continual light or dark treatments as shown by insignificant R/G ratio shift. Patterns of R/G ratio shift in symbiont LBs were also different between corals undergoing bleaching and continual light/dark treatment. In the latter, there was little lipid accumulation in symbionts, with no resulting R/G ratio decrease. These results, demonstrating that the symbiotic status positively correlated with morphological and compositional changes of lipid bodies, not only highlight the pivotal role of LBs, but also implicate an involvement of lipid trafficking in regulating the endosymbiosis.
URI: http://hdl.handle.net/11455/50404
ISSN: 0722-4028
文章連結: http://dx.doi.org/10.1007/s00338-008-0462-8
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