請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/50409
標題: Constitution of stable artificial oil bodies with triacylglycerol, phospholipid, and caleosin
作者: Chen, M.C.M.
曾志正
Chyan, C.L.
Lee, T.T.T.
Huang, S.H.
Tzen, J.T.C.
關鍵字: artificial oil bodies
caleosin
oleosin
seed
stability
oleosin isoforms
lipid bodies
proteins
sesame
surface
seeds
purification
biogenesis
domain
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 52, Issue 12, Page(s) 3982-3987.
摘要: Seed oil bodies are lipid storage organelles of 0.5-2 mum in diameter and comprise a triacylglycerol matrix shielded by a monolayer of phospholipids and proteins. These proteins include abundant structural proteins, oleosins, and at least two minor proteins termed caleosin and steroleosin. This study examined if artificial oil bodies (AOBs) composed of triacylglycerol and phospholipid could be stabilized by oleosin, caleosin, or steroleosin. Our results showed that stabilization effects could be realized by oleosin or caleosin but not by steroleosin. The sizes of the AOBs constituted with oleosin (0.5-2 mum) or caleosin (50-200 nm) were similar to or 10 times smaller than those of the native oil bodies. Recombinant caleosin expressed in Escherichia coli also encapsulated AOBs with a size, topology, and stability comparable to those encapsulated with native caleosin. A proteinase K digestion indicated that caleosin anchored the AOl3s via its central hydrophobic domain of approximately 4 kDa. Isoelectrofocusing revealed that the isoelectric point of the caleosin-stabilized AOBs was pH 4.0. Aggregation of AOBs was observed at a pH lower than 4.5; thus, their stability and integrity were presumably contributed by surface caleosin via electronegative repulsion and steric hindrance. The caleosin-stabilized AOBs were thermostable up to 70 degreesC and potentially useful for biotechnological applications.
URI: http://hdl.handle.net/11455/50409
ISSN: 0021-8561
文章連結: http://dx.doi.org/10.1021/jf035533g
顯示於類別:生物科技學研究所

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