Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50413
標題: Molecular cloning of 11S globulin and 2S albumin, the two major seed storage proteins in sesame
作者: Tai, S.S.K.
曾志正
Wu, L.S.H.
Chen, E.C.F.
Tzen, J.T.C.
關鍵字: 11S globulin
2S albumin
seed
sesame
sulfur-rich protein
oil bodies
vicia-faba
indicum l
identification
methionine
legumin
rich
gene
cleavage
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 47, Issue 12, Page(s) 4932-4938.
摘要: Insoluble 11S globulin and soluble 2S albumin, conventionally termed alpha-globulin and beta-globulin, are the two major storage proteins and constitute 80-90% of total seed proteins in sesame. Two full-length cDNA clones were sequenced and deduced to encode sesame 11S globulin and 2S albumin precursors, respectively. Deduced amino acid composition reveals that 2S albumin, but not 11S globulin, is a sulfur-rich protein. Three abundant polypeptides of 50-60 kDa were resolved on SDS-PAGE when seed-purified 11S globulin was prepared in nonreducing conditions. Immunological analysis suggests that these three polypeptides are encoded by homologous genes. Immunodetection on the overexpressed protein of the 11S globulin clone in Escherichia coli indicates that this clone encodes the precursor protein of one of the three purified 11S globulin polypeptides.
URI: http://hdl.handle.net/11455/50413
ISSN: 0021-8561
文章連結: http://dx.doi.org/10.1021/jf990366z
Appears in Collections:生物科技學研究所

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