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標題: Protective effects of sesamin and sesamolin on hypoxic neuronal and PC12 cells
作者: Hou, R.C.W.
Huang, H.M.
Tzen, J.T.C.
Jeng, K.C.G.
關鍵字: PC12 cells
mitogen-activated protein kinases
reactive oxygen species
focal cerebral-ischemia
activated protein-kinase
reperfusion injury
induced apoptosis
transgenic mice
期刊/報告no:: Journal of Neuroscience Research, Volume 74, Issue 1, Page(s) 123-133.
摘要: Reactive oxygen species (ROS) are important mediators of a variety of pathological processes, including inflammation and ischemic injury. The neuroprotective effects of sesame antioxidants, sesamin and sesamolin, against hypoxia or H2O2-induced cell injury were evaluated by cell viability or lactate dehydrogenase (LDH) activity. Sesamin and sesamolin reduced LDH release of PC12 cells under hypoxia or H2O2-stress in a dose-dependent manner. Dichlorofluorescein (DCF)-sensitive ROS production was induced in PC12 cells by hypoxia or H2O2-stress but was diminished in the presence of sesamin and sesamolin. We evaluated further the role of mitogen-activated protein kinases (MAPKs) and caspase-3 in hypoxia-induced PC12 cell death. Extracellular signal-regulated protein kinase (ERK) 1, c-jun N-terminal kinase (JNK), and p38 MAPKs of signaling pathways were activated during hypoxia. We found that the inhibition of MAPKs and caspase-3 by sesamin and sesamolin correlated well with the reduction in LDH release under hypoxia. Furthermore, the hypoxia-induced apoptotic-like cell death in cultured cortical cells as detected by a fluorescent DNA binding dye was reduced significantly by sesamin and sesamolin. Taken together, these results suggest that the protective effect of sesamin and sesamolin on hypoxic neuronal and PC12 cells might be related to suppression of ROS generation and MAPK activation. (C) 2003 Wiley-Liss, Inc.
ISSN: 0360-4012
Appears in Collections:生物科技學研究所



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