請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/50425
標題: Cloning and immunolocalization of an antifungal chitinase in jelly fig (Ficus awkeotsang) achenes
作者: Li, Y.C.
曾志正
Yang, Y.C.
Hsu, J.S.F.
Wu, D.J.
Wu, H.H.
Tzen, J.T.C.
關鍵字: jelly fig (Ficus awkeotsang)
Moraceae
Achene
Chitinase
Collelotrichum gloeosporioides
pericarp
acidic pectin methylesterase
molecular characterization
oil bodies
expression
fruit
proteins
tomato
purification
localization
resistance
期刊/報告no:: Phytochemistry, Volume 66, Issue 8, Page(s) 879-886.
摘要: A 30-kDa protein extracted from the pericarpial portion of jelly fig (Ficus awkeotsang Makino) achenes has been identified as a thermostable chitinase based on its enzymatic activity. A cDNA fragment encoding the precursor protein (including a cleavable signal sequence) of this chitinase was obtained by PCR cloning, and subsequently confirmed by immunological recognition of its over-expressed protein in Escherichia coli. Homology modeling predicted that this thermostable chitinase ill jelly fig achenes comprised a stable (βα)(8) barrel fold with three pairs of disulfide linkage. Immunostaining indicated that this chitinase was exclusively localized in the pericarpial region but not in the seed cells where bulky protein bodies and massive oil bodies were accumulated. Spore germination of Colletotrichum gloeosporioides, a common post-harvest pathogen infecting ripening fruit of jelly fig and many other fruits, was inhibited by this chitinase purified from achenes. It is suggested that the biological function of the thermostable chitinase ill the pericarp of jelly fig achenes is to protect the nutritive seeds from fungal attack during fruit ripening. © 2005 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/50425
ISSN: 0031-9422
文章連結: http://dx.doi.org/10.1016/j.phytochem.2005.02.015
顯示於類別:生物科技學研究所

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