請用此 Handle URI 來引用此文件: http://hdl.handle.net/11455/50430
標題: Elevation of oil body integrity and emulsion stability by polyoleosins, multiple oleosin units joined in tandem head-to-tail fusions
作者: Scott, R.W.
曾志正
Winichayakul, S.
Roldan, M.
Cookson, R.
Willingham, M.
Castle, M.
Pueschel, R.
Peng, C.C.
Tzen, J.T.C.
Roberts, N.J.
關鍵字: oleosin
polyoleosin
oil body
emulsion
recombinant protein
transgenic
freezing tolerance
germination
safflower carthamus-tinctorius
central hydrophobic domain
arabidopsis-thaliana
escherichia-coli
lipid bodies
floral dip
proteins
seeds
purification
expression
期刊/報告no:: Plant Biotechnology Journal, Volume 8, Issue 8, Page(s) 912-927.
摘要: We have successfully created polyoleosins by joining multiple oleosin units in tandem head-to-tail fusions. Constructs encoding recombinant proteins of 1, 3 and 6 oleosin repeats were purposely expressed both in planta and in Escherichia coli. Recombinant polyoleosins accumulated in the seed oil bodies of transgenic plants and in the inclusion bodies of E. coli. Although polyoleosin was estimated to only accumulate to < 2% of the total oil body protein in planta, their presence increased the freezing tolerance of imbibed seeds as well as emulsion stability and structural integrity of purified oil bodies; these increases were greater with increasing oleosin repeat number. Interestingly, the hexameric form of polyoleosin also led to an observable delay in germination which could be overcome with the addition of external sucrose. Prokaryotically produced polyoleosin was purified and used to generate artificial oil bodies and the increase in structural integrity of artificial oil bodies-containing polyoleosin was found to mimic those produced in planta. We describe here the construction of polyoleosins, their purification from E. coli, and properties imparted on seeds as well as native and artificial oil bodies. A putative mechanism to account for these properties is also proposed.
URI: http://hdl.handle.net/11455/50430
ISSN: 1467-7644
文章連結: http://dx.doi.org/10.1111/j.1467-7652.2010.00522.x
顯示於類別:生物科技學研究所

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