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標題: High-frequency shoot regeneration from leaf explants through organogenesis in bitter melon (Momordica charantia L.)
作者: Thiruvengadam, M.
Rekha, K.T.
Yang, C.H.
Jayabalan, N.
Chung, I.M.
關鍵字: Adventitious shoots
Growth regulators
Organogenic callus
efficient plant-regeneration
in-vitro organogenesis
cucumis-sativus l
citrullus-vulgaris schrad
squash cucurbita-pepo
somatic embryogenesis
期刊/報告no:: Plant Biotechnology Reports, Volume 4, Issue 4, Page(s) 321-328.
摘要: An efficient protocol for in vitro organogenesis was achieved from callus-derived immature and mature leaf explants of Momordica charantia, a very important vegetable and medicinal plant. Calluses were induced from immature leaf explants excised from in vitro (15-day-old seedlings) mature leaf explants of vivo plants (45 days old). The explants were grown on Murashige and Skoog (MS) medium with Gamborg (B5) vitamins containing 30 g l(-1) sucrose, 2.2 g l(-1) Gelrite, and 7.7 mu M naphthalene acetic acid (NAA) with 2.2 mu M thidiazuron (TDZ). Regeneration of adventitious shoots from callus (30-40 shoots per explant) was achieved on MS medium containing 5.5 mu M TDZ, 2.2 mu M NAA, and 3.3 mu M silver nitrate (AgNO(3)). The shoots (1.0 cm length) were excised from callus and elongated in MS medium fortified with 3.5 mu M gibberellic acid (GA(3)). The elongated shoots were rooted in MS medium supplemented with 4.0 mu M indole 3-butyric acid (IBA). Rooted plants were acclimatized in the greenhouse and subsequently established in soil with a survival rate of 90%. This protocol yielded an average of 40 plants per leaf explant with a culture period of 98 days.
ISSN: 1863-5466
Appears in Collections:生物科技學研究所



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