Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50468
標題: The AAUAAA motif of Bamboo mosaic virus RNA is involved in minus-strand RNA synthesis and plus-strand RNA polyadenylation
作者: Chen, I.H.
蔡慶修
Chou, W.J.
Lee, P.Y.
Hsu, Y.H.
Tsai, C.H.
徐堯煇
關鍵字: 3' untranslated region
helicase-like domain
synthesis in-vitro
messenger-rna
poly(a) tail
cytoplasmic polyadenylation
viral-rna
nucleotide-sequence
potexvirus rna
plant-viruses
期刊/報告no:: Journal of Virology, Volume 79, Issue 23, Page(s) 14555-14561.
摘要: Bamboo mosaic virus (BaMV) has a single-stranded positive-sense RNA genome with a 5'-cap structure and a 3' poly(A) tail. Deleting the internal loop that contains the putative polyadenylation signal (AAUAAA) in the 3' untranslated region (UTR) of BaW genomic RNA appeared to diminish coat protein accumulation to 2% (C. P. Cheng and C. H. Tsai, J. Mol. Biol. 288:555-565, 1999). To investigate the function of the AAUAAA motif, mutations were introduced into an infectious BaMV cDNA at each residue except the first nucleotide. After transfection of Nicotiana benthamiana protoplasts with RNA transcript, the accumulations of viral coat protein and RNAs were determined. Based on the results, three different categories could be deduced for the mutants. Category 1 includes two mutants expressing levels of the viral products similar to those of the wild-type virus. Six mutations in category 2 led to decreased to similar levels of both minus-strand and genomic RNAs. Category 3 includes the remaining seven mutations that also bring about decreases in both minus- and plus-strand RNA levels, with more significant effects on genomic RNA accumulation. Mutant transcripts from each category were used to infect N. benthamiana plants, from which viral particles were isolated. The genomic RNAs of mutants in category 3 were found to have shorter poly(A) tails. Taken together, the results suggest that the AAUAAA motif in the 3' UTR of BaMV genomic RNA is involved not only in the formation of the poly(A) tail of the plus-strand RNA, but also in minus-strand RNA synthesis.
URI: http://hdl.handle.net/11455/50468
ISSN: 0022-538X
文章連結: http://dx.doi.org/10.1128/jvi.79.23.14555-14561.2005
Appears in Collections:生物科技學研究所

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