Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50477
標題: A conserved secondary structure in the hypervariable region at the 5 ' end of Bamboo mosaic virus satellite RNA is functionally interchangeable
作者: Yeh, W.B.
徐堯煇
Hsu, Y.H.
Chen, H.C.
Lin, N.S.
葉文斌
關鍵字: hypervariable region
Bamboo mosaic virus
satellite RNA
triple-gene-block
molecular epidemiology
phylogenetic analysis
nucleotide-sequence
potexvirus rna
plant-viruses
helper virus
genomic rna
replication
evolution
期刊/報告no:: Virology, Volume 330, Issue 1, Page(s) 105-115.
摘要: Satellite RNA (satRNA) associated with Bamboo mosaic virus (BaMV) is dependent on BaMV for replication and encapsidation. Molecular analyses of total RNA extracted from bamboo species collected worldwide revealed that 26 out of 61 BaMV isolates harbored satBaMV. Among them, two phylogenetically distinguishable groups, A and B, with a genetic diversity of 6.9 +/- 0.7% were identified. Greatest sequence diversity occurred in the 5' untranslated region (UTR) that contained one hypervariable region with variations of up to 20.7%. Concurrent covariations in the 5' hypervariable sequences support the existence of a conserved apical hairpin stem-loop structure, which was earlier mapped by enzymatic probings and functional analyses [Annamalai, P., Hsu, Y.H., Liu, Y.P., Tsai, C.H., Lin, N.S., 2003. Structural and mutational analyses of cis-acting sequences in the 5'-untranslated region of satellite RNA of bamboo mosaic potexvirus. Virology 311 (1), 229 239]. Furthermore, chimeric satBaMVs generated by interchanging the hypervariable region between groups A and B demonstrated the replication competence of satBaMV isolates in Nicotiana benthamiana protoplasts co-inoculated with BaMV RNA. The results suggest that an evolutionarily conserved secondary structure exists in the hypervariable region of 5' UTR of satBaMV. (C) 2004 Elsevier Inc. All rights reserved.
URI: http://hdl.handle.net/11455/50477
ISSN: 0042-6822
文章連結: http://dx.doi.org/10.1016/j.virol.2004.09.025
Appears in Collections:生物科技學研究所

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