Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50480
標題: Characterization of Oncidium 'Gower Ramsey' Transcriptomes using 454 GS-FLX Pyrosequencing and Their Application to the Identification of Genes Associated with Flowering Time
作者: Chang, Y.Y.
呂維茗
Chu, Y.W.
Chen, C.W.
Leu, W.M.
Hsu, H.F.
Yang, C.H.
楊長賢
朱彥煒
關鍵字: CONSTANS
Expressed sequence tag
Flowering time
Oncidium orchid
Photoperiod flowering pathway
Transcriptome
floral meristem identity
mads box genes
plant circadian clock
arabidopsis-thaliana
blue-light
orchid
transition
expression
evolution
sequences
期刊/報告no:: Plant and Cell Physiology, Volume 52, Issue 9, Page(s) 1532-1545.
摘要: Oncidium 'Gower Ramsey' is a valuable and successful commercial orchid for the floriculture industry in Taiwan. However, no genome reference for entire sequences of the transcribed genes currently exists for Oncidium orchids, to facilitate the development of molecular biological studies and the breeding of these orchids. In this study, we generated Oncidium cDNA libraries for six different organs: leaves, pseudobulbs, young inflorescences, inflorescences, flower buds and mature flowers. We utilized 454-pyrosequencing technology to perform high-throughput deep sequencing of the Oncidium transcriptome, yielding > 0.9 million reads with an average length of 328 bp, for a total of 301 million bases. De novo assembly of the sequences yielded 50,908 contig sequences with an average length of 493 bp from 796,463 reads and 120,219 singletons. The assembled sequences were annotated using BLAST, and a total of 12,757 and 13,931 unigene transcripts from the Arabidopsis and rice genomes were matched by TBLASTX, respectively. A Gene Ontology (GO) analysis of the annotated Oncidium contigs revealed that the majority of sequenced genes were associated with 'unknown molecular function', 'cellular process' and 'intracellular components'. Furthermore, a complete flowering-associated expressed sequence that included most of the genes in the photoperiod pathway and the 15 CONSTANS-LIKE (COL) homologs with the conserved CCT domain was obtained in this collection. These data revealed that the Oncidium expressed sequence tag (EST) database generated in this study has sufficient coverage to be used as a tool to investigate the flowering pathway and various other biological pathways in orchids. An OncidiumOrchidGenomeBase (OOGB) website has been constructed and is publicly available online (http://predictor.nchu.edu.tw/oogb/)
URI: http://hdl.handle.net/11455/50480
ISSN: 0032-0781
文章連結: http://dx.doi.org/10.1093/pcp/pcr101
Appears in Collections:生物科技學研究所

文件中的檔案:

取得全文請前往華藝線上圖書館



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.