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|標題:||Biological and biochemical detection techniques for glufosinate|
|期刊/報告no：:||Weed Science, Volume 54, Issue 3, Page(s) 413-418.|
|摘要:||Biological and biochemical methods, based on glufosinate inhibitory effects on plant growth and nitrogen metabolism, were examined for their applications to detect this herbicide. Dose-response analysis of radicle growth inhibition showed that, among six vegetables tested, Chinese mustard and edible amaranth were the most sensitive to glufosinate. Field mustard and cruciferous Ching-Geeng were found to be more sensitive to this herbicide than the other four vegetables when three-leaf-seedlings were tested in another bioassay. In three-leaf seedlings of Ching-Geeng, accumulation of ammonium, a biochemical marker for glufosinate toxicity because of its inhibition of glutamine synthetase, showed a linear regression to the log-transformed concentrations of glufosinate ranging from 7.5 X 10(-5) to 1.5 X 10(-3) M. For the detection of glufosinate lower than 7.5 X 10(-6) M, a linear regression was observed between ammonium accumulation and the applied concentration, instead of the log-trans- formed value, of glufosinate. A similar relationship was observed between the accumulation in Ching-Geeng seedlings of glyoxylate, another biochemical marker, and glufosinate but with a narrower range than that for ammonium accumulation. The applicability of ammonium accumulation in three-leaf seedlings of Ching-Geeng to detect glufosinate residue in water and soil was confirmed by high-performance liquid chromatography (HPLC).|
|Appears in Collections:||農藝學系|
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