Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50662
標題: Biological and biochemical detection techniques for glufosinate
作者: 王慶裕
Zhou, X.H.
Wang, C.Y.
關鍵字: bioassay
biochemical marker
herbicide detection
herbicide persistence
glutamine-synthetase activity
ammonium accumulation
liquid-chromatography
rapid-determination
zea-mays
phosphinothricin
photosynthesis
glyphosate
growth
acid
期刊/報告no:: Weed Science, Volume 54, Issue 3, Page(s) 413-418.
摘要: Biological and biochemical methods, based on glufosinate inhibitory effects on plant growth and nitrogen metabolism, were examined for their applications to detect this herbicide. Dose-response analysis of radicle growth inhibition showed that, among six vegetables tested, Chinese mustard and edible amaranth were the most sensitive to glufosinate. Field mustard and cruciferous Ching-Geeng were found to be more sensitive to this herbicide than the other four vegetables when three-leaf-seedlings were tested in another bioassay. In three-leaf seedlings of Ching-Geeng, accumulation of ammonium, a biochemical marker for glufosinate toxicity because of its inhibition of glutamine synthetase, showed a linear regression to the log-transformed concentrations of glufosinate ranging from 7.5 X 10(-5) to 1.5 X 10(-3) M. For the detection of glufosinate lower than 7.5 X 10(-6) M, a linear regression was observed between ammonium accumulation and the applied concentration, instead of the log-trans- formed value, of glufosinate. A similar relationship was observed between the accumulation in Ching-Geeng seedlings of glyoxylate, another biochemical marker, and glufosinate but with a narrower range than that for ammonium accumulation. The applicability of ammonium accumulation in three-leaf seedlings of Ching-Geeng to detect glufosinate residue in water and soil was confirmed by high-performance liquid chromatography (HPLC).
URI: http://hdl.handle.net/11455/50662
ISSN: 0043-1745
文章連結: http://dx.doi.org/10.1614/ws-05-155r.1
Appears in Collections:農藝學系

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