Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/5102
標題: 滲出水中添加生物製劑對覆土掩埋場甲烷氧化行為之研究
Enhancement by leachate with biological additive on methane oxidation in landfill cover soil
作者: 林鍾豪
Lin, Chung-Hao
關鍵字: methanotrophs
甲烷氧化菌
methane oxidation
bio-agent
landfill cover soil
leachate
甲烷氧化
生物製劑
掩埋場覆土
滲出水
出版社: 環境工程學系所
引用: 中文部份 行政院環保署。2001。國家通訊編撰計畫,九十年度期末報告。 行政院環保署。2002。聯合國氣候變化綱要公約國家通訊。 行政院環保署。2005 京都議定書生效後整體策略方向,2005全國能源會議 王義狄。1999。環境中甲烷氧化菌計數方法之研究。國立中興大學環境工程研究所碩士論文。 王凱中。1994。 垃圾掩埋場滲出水量之模式推估。國立台灣大學環境工程學研究所碩士論文。 周奮興。1998。以通量室監測封閉掩埋場甲烷排放量之研究。國立中興大學環境工程研究所碩士論文。 李季眉等。1988。微生物學實驗。國立中興大學環境工程系。 李季眉等。1997。環境微生物。中華民國環境工程學會印行。 李甘露。2002。添加有機污泥對掩埋場覆土甲烷氧化行為之研究。國立中興大學環境工程研究所碩士論文。 徐玟瑜。1999。掩埋場覆土中土壤水對甲烷氧化行為之研究。國立中興大學環境工程研究所碩士論文。 吳麗芬。1995。含可溶性甲烷單氧氧化酵素之甲烷氧化菌對三氯乙烯之分解。國立中興大學環境工程研究所碩士論文。 廖俊博。2002。南仁山古湖底泥甲烷氧化菌社會結構之研究。國立成功大學生物學研究所碩士論文。 劉惠珍。2001。添加銨鹽、尿素及硝酸鹽對掩埋場覆土甲烷氧化行為之研究。國立中興大學環境工程研究所碩士論文。 劉卜逢。2003。甲烷氧化菌在厭氧腐熟土中之動力活性探討。國立中興大學環境工程研究所碩士論文。 謝一誠。2002。掩埋場覆土中氣體濃度分佈與覆土甲烷氧化作用之研究。國立中興大學環境工程研究所碩士論文。 籃賢傳。2000。含水量對管柱中甲烷氧化行為影響之研究。國立中興大學環境工程研究所碩士論文。 鄭淑仁。1996。環境中甲烷氧化菌之分離及其對三氯乙烯的分解能力。國立中興大學環境工程研究所碩士論文。 段玄治。2002。工業用水的節約及再利用-以燁輝鋼鐵公司為例。屏東科技大學環境工程與科學系碩士論文。 陳思霖。2000。EDTA生物分解之研究。雲林科技大學環境與安全工程技術研究所碩士論文。 西文部份 Christensen, T. H., R. Cossu and R. Stegmamn, 1989, Sanitary Landfilling Process, Technology and Environmental Impact, 1st Ed., Academic Press, London, pp. 29-49. IPCC (2000), Good Practice Guidance and Uncertainty Management in National Tchobanoglous, G., H. Theisen and S. A. Vigil, 1993, Integrated Solid Waste Management. U. S. EPA (2000), Global Warming and Our Changing Climate, EPA 430-F-00-011. Amaral, J. A. and R. Knowles, 1997, "Inhibition of methane consumption in forest soils and pure cultures of methanotrophs by aqreous forest soil extracts," Soil Biology & Bio- chemistry, Vol. 29, No. 11/12, pp.1713-1720. Baetz, B. W. and Onysko, K. A, 1993, "Storage volume sizing for landfill leachate re- circulation system," Journal of Environmental Engineering Vol. 199, pp. 378-383. Bedard, C. and R. Knowles, 1989, "Physiology, biochemistry, and specific inhibitors of CH4, NH4+, and CO oxidation by methanotrophs and nitrifiers," Microbiological Review, Vol. 53, pp. 68-84. Bender, M. and R. Conrad, 1994, "Methane oxidation activity in various soils and fre- shwater sediment, occurrence characteristic vertical profiles and distribution on grain size fractions," Journal of geophysical research, Vol. 99, pp. 16531-16540. Bender, M. and R. Conrad, 1995, "Effect of CH4 concentrations and soil conditions on the induction of CH4 oxidation activity," Soil Biology & Biochemistry, Vol. 27, pp. 1517- 1527. Benstead, J. and G. M. King, 2001, "The effect of soil acidification on atmospheric me- thane uptake by a Maine forest soil," FEMS Microbiology Ecology, Vol. 34, pp. 207- 212. Benstead, J. and G. M. King, 1997, "Response of methanotrophic activity in forest soil to methane availability," FEMS Microbiology Ecology, Vol. 23, pp. 333-340. Boeckx, P., O. V. Cleemput and I. Villaralvo, 1996, "Methane emission from a landfil1 and the methane oxidising capacity of its covering soil," Soil Biology & Biochemistry, Vol. 28, pp. 1397-1405. Bogner, J. E., 1997, "Kinetics of methane oxidation in a landfill cover soil:temporal variations, a whole-landfill oxidation experiment, and modeling of net CH4 emission," Environmental Science and Technology, Vol. 31, pp. 2504-2514. Börjesson, G., I. Sundh, A. Tunlid and B. Svensson, 1998, "Methane oxidation in land- fill cover soil, as revealed by potential oxidation measurements and phospholipid fatty acid analyses," Soil Biology & Biochemistry, Vol. 30, pp. 1423-1433. Börjesson, G., I. Sundh and B. Svensson, 2004, "Microbial oxidation of CH4 at differ- rent temperatures in landfill cover soils," FEMS Microbiology Ecology, Vol. 48, pp. 305- 312. Berger, J., L. V. Fornes, C. Ott, J. Jager, B. Wawra and U. Zanke, 2005, "Methane oxidation in a landfill cover with capillary barrier," Waste Management, Vol.25, pp. 369- 373. Christensen, S., D. Anna and P. Anders, 2001, "Methane oxidation in Polish forest soils of contrasiting atmospheric pollution," Atmospheric Environment, Vol. 35, pp. 2795- 2798. Chida, K., Shen, G., Kodama, T., Minoda, Y., 1983, "Acidic polysaccharide production from methane by a new methane-oxidising bacterium H-2," Agricultural and Biological Chemistry, Vol. 47, pp. 65-89. Doedens, H. and Cord-Landwehr, K, 1989, "Leachate recirculation, " In Sanitary Land- filling: Process,Technology and Environmental Impact, (T. H. Christensen, R. Cossu and R. Stegmann, eds). London, U.K.: Academic press. El-Fadel, M., E. Bou-Zeid, W. Chahine and B. Alayli, 2002, "Temporal variation of leachate quality from pre-sorted and baled municipal solid waste with high organic and moisture content," Waste Management, Vol. 22, pp. 269-282. Farquhar, G. J. and Rovers, F. A., 1973, "Gsa production during refuse decomposition, water, air, and soil pollution," Vol. 2, pp. 483-495. Fang, C. and J. B. Mocrieff, 1998, "Simple and fast technique to measurement CO2 pro- file in soil," Soil Biology & Biochemistry, Vol. 30, pp. 2107-2112. Farquhar, G. J., 1989, "Leachate: production and characterization," Canadian Journal of Civil Engineering Vol. 16, pp. 317-25. Graham, D. W., D. G. Korich, R. P. LeBlance, N. A. Sinclair and R. G. Arnold, 1992, "Application of a calorimetric plate assay for soluble methane monooxygenase activity," Applied and Environment Microbiology, Vol. 58, No. 7, pp. 2231-2236. Gulledge, J., Y. Hrywna, C. Cavanaugh and P. A. Steudler, 2004, "Effect of long-term nitrogen fertilization on the uptake kinetics of atmospheric methane in tem- perate forest soils," FEMS Microbiology Ecology, Vol. 49, pp. 389- 400. Hanson, R. S. and T. E. Hanson, 1996, "Methanotrophic bacteria," Microbiological Review, Vol. 60, pp. 439-471. Hütsch, B. W., 2001," Methane oxidation, nitrification, and counts of methanotrophic bacteria in soils from a long term fertilization experiment," J. Plant Nutr. Soil Sci., Vol. 164, pp. 21-28. Hilger, H. A., D. F. Cranford and M. A. Barlaz, 2000, "Methane oxidation and micro- bial exopolymer production in landfill cover soil," Soil Biology & Biochemistry, Vol. 32, pp. 457-467. Hilger, H. A., Liehr, S.K. and M. A. Barlaz, 1999, "Exopolysaccharide control of me- thane oxidation in landfill cover soil," Journal of Environmental Engineering, Vol.125, pp. 1113-1123. Haubrichs, R. and R. Widmann, 2006, "Evaluation of aerated biofilter systems of microbial methane oxidation of poor landfill gas," Waste Management, Vol. 26, pp. 408-416. Iwamoto, T. and Nasu M., 2001, "Current bioremediation practice and perspective," Journal of Bioscience and Bioengineering, Vol. 92, No. 1, pp. 1-8. Kightley, D., D. B. Nedwell and M. Cooper, 1995, "Capacity for methane oxidation in landfill cover soils measured in laboratory-scale soil microcosms," Applied and Environ- mental Microbiology, Vol. 61, pp. 592-601. King, G. M. and S. Schnell, 1998, "Effects of ammonium and non-ammonium salt addi- tions on methane oxidation by Methylosinus trichosporium OB3b and maine forest soils," Applied and Environmental Microbiology, Vol. 64, pp. 253-257. Koerner, G. R. and Koerner, R. M., 1995, "Permeability of granular drainage mate- rial," Presented at the U.S. EPA bioreactor landfill design and operation seminar, Wil- mington, Delaware, U.S.A. Kruse, C. W. and N. Iversen, 1995, "Effect of plant succession, ploughing, and fertili- zation on the microbiological oxidation of atmospheric methane in a heathland soil," FEMS Microbiology Ecology, Vol. 18, pp. 121-128. Linton, J. D., Watts, P. D., Austin, R. M., Haugh, D.E. and Neikus, H. G. D., 1986, "The energetics and kinetics of extracellular polysaccharide production from methanol by microorganisms possessing different pathways of Cl assimilation," Journal of General Microbiology, Vol. 132, pp. 779-778. Lo, Irene M.-C., 1996, "Characteristics and treatment of leachates from domestic land- fills," Environment International," Vol. 22, No. 4, pp. 433-442. Mosher, B. W., P. M. Czepiel, R. C. H. H. Shorter, C. E. Dolb, J. B. M. Allwine and B. K. Lamb, 1997, "Methane Emissions at Nine Landfill sites in The Northeastern United States," Environmental Science and Technology, Vol. 33, pp. 2088-2094. Milich, L., 1999, "The role of methane in global warming: where might mitigation strate- gies be focused," Global Environment Change, Vol. 9, pp.179-201. Macdonald, J. A., D. Fowler, K. J. Hargreaves and U. Skiba, 1998, "Methane emission rates from a northern wetland response to temperature, water table and transport," Atmos- phere Environment, Vol. 32, pp. 3219-3227. Miller, W. L., Townsend, T., Earle, J., Lee, H. and Reinhart, D. R., 1994, "Leachate recycle and the Augmentation of biological decomposition at municipal solid waste landfill," Presented at the second annual research symposium, Florida Center for solid and hazardous waste management, Tampa, Florida, U.S.A. Mer, J. L. and P. Roger, 2001, "Production, oxidation, emission and consumption of methane by soils: A review," European Journal of Soil Biology, Vol. 37, pp. 25-50. Mishra, S. R., K. Bharati and N. Sethunathan, 1999, "Effects of heavy metals on me- thane production in tropical rice soils," Ecotoxicology and Environmental Safety, Vol. 44, pp. 129-136. Mohanty, S. R., K. Bharati, N. Deepa, V. R. Rao and T. K. Adhya, 2000, "Influence of heavy metals on methane oxidation in tropical rice soils," Ecotoxicology and Environ- mental Safety, Vol. 47, pp. 277-284. Murrell, J. C., I. R. McDonald and D.G. Bourne, 1998, "Molecular methods for the study of methanotroph ecology," FEMS Microbiology Ecology, Vol. 33, pp. 2088-2094. Murrell, J. C., I. R. McDonald and B. Gilbert, 2000, "Regulation of expression of methane monooxygenases by copper ions," Trends in Microbiology, Vol. 8, pp. 221-225. Mor, S., Visscher, A. D., K. Ravindra, R.P. Dahiya, A. Chandra and O. V. Cleemput, 2006, "Induction of enhanced methane oxidation in compost: Temperature and moisture response," Waste Management, Vol. 26, pp. 381-388. Pohland, F. G. and Harper, S. R., 1986, "Critical Review and Summary of leachate and Gas Production From Landfills," EPA/600/2-86/073. Cincinnati, OH, U.S.A.: U.S. Environment Protection Agency. Park, J. W. and H. C. Shin, 2001, "Surface emission of landfill gas from solid waste landfill," Atmospheric Environment, Vol. 35, pp. 3445-3451. Prieme, A. and E. Flemming, 2001, "Five pesticides decrease oxidation of atmospheric methane in a forest soil," Soil Biology & Biochemistry, Vol. 33, pp. 831-835. Reinhart, D. R. and A. Basel Al-Yousfi, 1996, "The impact of leachate recirculation on municipal solid waste landfill operating characteristics," Waste Management, Vol. 14, pp. 337-346. Reinhart, D. R., 1996, "Full-scale experiences with leachate recirculating landfill: case studies," Waste Management, Vol. 14, pp. 347-365. Saari, A., R. Rinnan and P. J. Martikainen, 2004, "Methane oxidation in boreal forest soils: kinetics and sensitivity to pH and ammonium," Soil Biology & Bio- chemistry, Vol. 36, pp. 1037-1046. Streese, J. and R. Stegmann, 2003, "Micorbial oxidation of methane form old landfills in biofilters," Waste Management, Vol. 23, pp. 573-580. Sitaula, B. K., S. Hansen, J. I. B. Sitaula and L. R. Bakken, 2000, "Methane oxidation potentials and fluxes in agricultural soil: Effects of fertilization and soil compaction," Biogeochemistry, Vol. 48, pp. 323-339. Tatsi, A. A. and A. I. Zouboulis, 2002, "A field investigation of the quantity and quality of leachate from a municipal solid waste landfill in a Mediterranean climate (Thessaloniki, Greece)," Advances in Environmental Research, Vol. 6, pp. 207-219. Tolaymat, T., 2005, "Bioreactor landfill design," The international workshop of bioreactor and methane recovery technologies on landfill, Taipai Taiwan. Visscher, A. D. and O. V. Cleemput, 2003, "Induction of enhanced CH4 oxidation in soils: NH4+ inhibition patterns," Soil Biology & Biochemistry, Vol. 35, pp. 907-913. Visscher, A. D., D. Thomas, P. Boeckx and O. V. Cleemput, 1999, "Methane oxidation in simulated landfill cover soil environments," Environmental Science and Technology, Vol. 33, pp. 1854-1859. Visscher, A. D., M. Schippers, and O. V. Cleemput, 2001, "Short - term kinetic res- ponse of enhanced methane oxidation in landfill cover soils to environmental factors," Biology and Fertility of Soils, Vol. 33, pp. 231-237. Visvanathane, C., J. P. A. Helttiaratchi and J. S. Wu, 1999, "Methanotrophic activities in tropical landfill cover soils – effects of temperature , moisture content and methane concentration," Waste Management & Research, Vol. 17, pp. 313-323. Whalen, S. C., W. S. Reeburch and K.A. Sandbeck, 1990, "Rapid methane oxidation in a landfill cover soil," Americian Soicety for Microbiology, Vol. 56, pp. 3404-3411. Wang, Z. P. and P. Ineson, 2002, "Methane oxidation in temperate coniferous forest soil: effects of inorganic N," Soil Biology & Biochemistry, Vol. 35, pp. 427-433. Whittenbury, R., and H. Dalton, 1981, "The methylotrophic bacteria," pp.894-902. In M. Starr, H. Stolp, H. G. Truper, A. Balows and H. G. Schlegel(ed.), The prokaryotes Vol.1. Spring-Verlag, New York. Wilshusen, J. H., J. P. A. Hettiaratchi and V. B. Stein, 2004, "Long-term behavior of passively aerated compost methanotrophic biofilter columns," Waste Management, Vol. 49, pp. 389-400. Yang, S. S. and H. L. Chang, 1998, "Effect of environment conditions on methane pro- duction and emission from paddy soil," Agriculture Ecosystems & Environment, Vol. 69, pp. 69-80. Yazdani, R., 2005, "Full-scale bioreactor landfill at Yolo County Central landfill," The international workshop of bioreactor and methane recovery technologies on landfill, Taipai Taiwan.
摘要: 本研究希望藉由掩埋場滲出水中添加生物製劑返送回掩埋場覆土,作為控制覆土的含水量同時刺激甲烷氧化菌活性之功用,減少掩埋場甲烷逸散到大氣中,降低掩埋場甲烷對溫室效應的衝擊,減緩全球溫室效應的持續惡化。 實驗分成批次實驗與管柱實驗兩部份進行。批次實驗的結果顯示以土壤含水量而言,乾燥的10%含水量土壤甲烷氧化作用並不明顯,接近飽和態的35%土壤甲烷氧化作用偏低,最適於甲烷氧化作用的土壤含水量應接近15%~25%。相同含水量下的甲烷氧化速率以添加生物製劑滲出水>滲出水>去離子水,以25%最佳含水量為例,其最大甲烷氧化速率大小分別為879、605、321(nmole g-1 DW soil hr-1)。甲烷氧化作用的持續性也以添加生物製劑滲出水>滲出水>去離子水。以25%含水量為例,批次實驗平均甲烷氧化速率大小分別為629.1、375.0、156.2(nmole g-1 DW soil hr-1)。 由甲烷氧化速率的變化與土壤及滲出水基本性質變化的比較,推測生物製劑具有加速滲出水中有機物礦化的作用,可減短滲出水中有機物對甲烷氧化作用抑制的時間。滲出水中的銨氮於本實驗中具有刺激甲烷氧化作用的功能,提升甲烷氧化作用。甲烷氧化菌菌數會經由馴養而增加,但與甲烷氧化能力並沒有絕對的相關性。 管柱實驗的結果顯示,當適量的水分加入土壤表面時(管柱整體含水量約在15%),具有刺激乾燥土壤中甲烷氧化菌活性的能力,提升甲烷氧化的能力。此時如同批次實驗的結果,生物製劑與滲出水具有增加甲烷氧化作用的效果。但當大量的水分加入後(管柱整體含水量約在25%),反而會造成管柱甲烷氧化作用能力下降,此時生物製劑與滲出水的添加無法具有增加甲烷氧化作用的能力。 由管柱氣體縱深分佈顯示,當大量的水分加入土壤後,使得管柱中甲烷氧化作用的區域由深度約10-40cm上移至深度10cm處,且由土壤基本性質縱深分佈顯示,生物製劑與滲出水的添加,如同批次實驗,具有刺激甲烷氧化菌活性的功用。因此推論,氧氣應當為影響掩埋場覆土中甲烷氧化作用的主要因數之ㄧ,添加過多的水份於土壤會造成土壤空隙的阻塞,因此使得氧氣傳輸能力的下降。土壤中氧氣的缺乏進而造成甲烷氧化作用的下降,而生物製劑與滲出水需在氧氣充足下,才具有增加甲烷氧化作用的能力。
The aim of this research was to investigate the potential use of the landfill leachate with biological additive to control the water content of landfill cover soil, and to stimulate the methanotrophic activity at the same time. This approach will enhance the methane oxidation in landfill cover soil. Therefore, the methane emission to atmosphere at landfills will decrease for the mitigation of the greenhouse effects. The experiments conducted in this study included both batch and column tests. The results of the batch experiment show that the methane oxidation was not obvious in the dry soil at a water content of 10%, that methane oxidation was on the low side when the soil was close to the saturation level at 35%, and that the water content most suitable for the methane oxidation should lie in the range of 15%-25%. Besides, the methane oxidation rates under the suitable water content was leachate with bio-additive > leachate> deionized water. At the best water contents of 25%, the maximum methane oxidation rates was 879, 605, and 321 nmole(g of dry soil)-1hr-1, respectively. The continuation of the methane oxidation was leachate with bio-additive > leachate> deionized water. At the best water contents of 25%, the average methane oxidation rates was 629, 375 and156 nmole (g of dry soil) -1 hr-1, respectively. The trends in the change of methane oxidation rates suggest that the role of the bio-agent could have enhanced the mineralization of organic matter in the leachate. That resulted in a shorter period of the time that the organic matter had suppressed the methane oxidation. In this experiment, the ammonium nitrogen in the leachate stimulated the activity of methanotrophs, and improved the rates of methane oxidation. Although the most probable number of methanotrophs increased via incubation, but had not absolute correlation with the oxidative rate of methane. The results of the column experiment shows that after suitable moisture irrigation (water content close to 15%) the methane oxidation rate was higher than before irrigation. Like the results of batch experiment, both the bio-agent and the leachate had the function to enhance methane oxidation. But after excessive moisture irrigation (water content higher than 25%), the methane-oxidation ability of the column decreased; and the bio-agent and leachate had no longer the function to enhance the methane oxidation. Gas concentration profiles indicated that after a period of moisture irrigation, the methane oxidation took place in about 10cm depth, compared with 10-40cm before irrigation. The data of biological parameters indicated that the bio-additive and leachate had the function to enhance methane oxidation which is the same as in the batch experiment. It can be assumed that oxygen is one of the most important limiting factors for the methane oxidation in landfill cover soil. An excessive irrigation caused the blocking of soil pores which limited the influx of oxygen. The shortage of oxygen in soil caused the decline of methane oxidation. It is concluded that both the bio-additive and leachate had the function to enhance methane oxidation when oxygen was not a limiting factor in landfill cover soil.
URI: http://hdl.handle.net/11455/5102
其他識別: U0005-0208200614330700
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