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Antioxidant and Antimutagenic Properties of Curcuma zedoaria
精油進一步以矽膠管柱層析分離，以不同比例之正戊烷及乙醚沖提所得之五個區分物中以區分物3及區分物4有較佳的抗氧化性。區分物4中則又以5 - isopropylidene- 3, 8 — dimethyl - 1(5H) - azulenone為主要成分。
ABSTRACT The rhizome of the plant Curcuma zedoaria, a topical herb of the Zingiberaceae family native to southern Asia. The Chinese traditional medicine Curcuma zedoaria ROSC. has been clinically used for the treatment of cervical cancer. Many of its components have reputed medicinal properties that have been demonstrated that the extracts and fractions from the dried rhizome of C. zedoaria used in traditional medicine possess anti-inflammatory, antifungal, and antitumor activities. However, the study of its antioxidant activity and antioxidant compounds had not been reported. The objectives of this research were to study the antioxidative activities of extracts from C. zedoaria ROSC simultaneous steam distillation-solvent extraction, different solvent extraction, supercritical fluid extraction and water extraction. This research was also investigate antioxidiative properties of methanolic extracts, water extract, essential oil and supercritical fluid extracts from the rhizome of C. zedoaria, such as reducing power, scavenging effect on 1,1-diphenyl-2-picryl hydrazyl (DPPH●) radical, hydroxyl radical and chelating effect on ferrous ions. C. zedoaria ROSC contained hight carbohydrate contents. The yield of essential oil with simultaneous steam distillation-solvent extraction was very low，but it showed 70-87% inhibition on peroxidation of linoleic acid, and the antioxidative activities of essential oil were better than ascorbic acid. The 36 components of essential oil had been identified by GC-MS, including 17 teperenes, 14 alcohols and 5 ketones. The extracts of supercritical fluid extraction showed the worse antioxidative activities than essential oil, and there were less intense of odor than essential oil. The 31 components of supercritical fluid extracts had been identified by GC-MS, including 15 teperenes, 11 alcohols and 5 ketones. The content of terpene and sesquiterpene might be the main factor to influence antioxidative activities between them. The essential oil was further subjected to silica gel column chromatography eluted with various ratios of solvent′s composites from pentane and ether. The III and IV fractions showed markedly antioxidative activities in 5 separated fractions obtained from silica gel column chromatography. Methanolic and water extracts of C. zedoaria showed 80-94% inhibition on peroxidation of linoleic acid, and the antioxidative activities of them were better than ascorbic acid. Them exhibited the good effect not only in antioxidative activities, but also in other antioxidative properties, such as reducing power, scavenging effect on 1,1-diphenyl-2-picryl hydrazyl (DPPH●) radical, hydroxyl radical and chelating effect on ferrous ions. The antioxidative characteristics of methanolic extracts from C. zedoaria were investigated. The methanolic fractions was further subjected to silica gel column chromatography eluted with various ratios of solvent′s composites from hexane, ethyl acetate and methanol. The VI and VIII fractions showed markedly antioxidative activities in 8 separated fractions obtained from silica gel column chromatography. The antimutageneic characteristics of methanolic extracts from C. zedoaria were investigated. The methanolic and water extracts from C. zedoaria showed markedly inhibition on the mutagenicity of IQ、B[a]P and 4-NQNO to S. typhimurium TA97、TA98、TA100 and TA102. Keyword: Curcuma zedoaria, essential oil, antioxidant properties, antimutagenic properties
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