Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/5156
標題: 以人類乳癌細胞研究微生物分解對環境雌性激素干擾效應之影響
Investigation of the effects of microbial degradation on the (anti)estrogenicity of environmental estrogens in human breast cancer cells
作者: 施聖彰
Shih, Sheng-Chang
關鍵字: E-Screen
微生物分解
microbial degradation
estrogens
(anti)estrogenicity
雌性激素
干擾效應
出版社: 環境工程學系所
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摘要: 本研究運用活體外(in vitro)生物檢測之細胞增生分析法(Cell Proliferation Assay,CPA)之中,以人類乳癌細胞增生分析法(Estrogen-screen assay)評估化學物質之雌性激素各種干擾效應,檢驗微生物分解對環境雌性激素物質的干擾效應,包括酚甲烷(bisphenol-A,BPA) 雌性激素促進效應、加保利(carbaryl)之雌性激素促進及抑制效應之影響。 本研究中以人類乳癌細胞增生分析法之實驗結果確認: 1.酚甲烷具有雌性激素促進效應,而為雌性激素干擾效應之促進物質。在濃度範圍(10-5M~10-8M)內,E-screen與酵母菌雌性激素篩選法(yeast estrogen screen, YES)結果相當一致。 2.加保利在濃度範圍(10-8M~10-6M)時有約二倍的E2促進效應,在高濃度(10-4M)以上時有細胞毒性導致較低E2促進效應(0.26、0.03<2.40),在較低濃度範圍(10-12M~10-9M)內為具雌性激素抑制物質。 3.酚甲烷細菌分解液在低濃度範圍(10-5M~10-8M)時有較高E2促進效應,在高濃度範圍(>10-5M)未見到有細胞毒性,而在濃度範圍(1.~2.*10-5M)內,E-screen與YES結果相當接近。 4.固相萃取之酚甲烷細菌分解液在濃度範圍內,有較低之E2促進效應,但趨勢相符,在低濃度範圍(10-8M~10-9M)內,E-screen與YES結果不同。 5.加保利細菌分解液細胞抑制效應在實驗濃度範圍內,從整體看時有較弱的E2抑制效應,可看出E-screen與YES結果趨勢相同,數值結果較為不同。 本研究結果證實,微生物分解對不同種類之雌性激素干擾物質之降解作用應個別逐一篩檢。E-screen與YES測試結果雖有差異但大體而言相當一致。 因此運用微生物分解作為生物處理方法,分解具環境荷爾蒙效應之有機污染物,其成效之評估有必要進一步研究及釐清。
The primary purpose of this study is to apply the Estrogen screening assay (E-screen) to evaluate the effects of microbial degradation on the (anti)estrogenic effects of environmental estrogens, including bisphenol-A and carbaryl. Results from the E-screen analyses indicated that: 1. Bisphenol-A is a potent estrogenic chemical at concentrations ranging from 10-5M~10-8M. Results from the E-screen analyses are comparable with those reported by the yeast estrogen screen (YES) assay. 2. Carbaryl alone displayed estogenic effects at concentrations ranging from 10-8M to 10-6M whereas at concentrations 10-4M of above cytotoxic response was detected. Co-treatment of carbaryl (10-12M~10-9M) the estrogenic effects of endogenous estrogen was inhibited. This evidence suggests that carbaryl possesses both estrogenic and anti-estrogenic effects as assayed by E-screen. 3. The medium derived from microbial degradation of BSA possess estrogenic effects at concentrations ranging from10-5M to10-8M. Results from the E-screen analyses are comparable with those reported by the yeast estrogen screen (YES) assay (1.~2*10-5M). 4. The extracts derived from microbial degradation of BSA after solid phase extraction displayed estrogenic effects at high concentrations ranging from 10-5M to 10-8M. 5. The extracts from the microbial degradation of carbaryl displayed persistant anti-estrogenic effects. Overall, results from our investigation suggest that the impact of microbial degradation processes on the activity of environmental estrogens should be carefully examined and should not be considered solely as a detoxification processes. In general, the experiment results of E-screen and YES are comparable although with some differences. So, the effects of using microbes as the method for microbial degradation to degrade the organic pollutant with Enviromental Hormones effects need further study and distinction.
URI: http://hdl.handle.net/11455/5156
其他識別: U0005-1408200623360500
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1408200623360500
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