Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/51932
標題: 大本山葡萄抗腸病毒活性及活性成分之研究
Anti-enterovirus activity and active compounds of Ampelopsis brevipedunculata (Maxim.) Trautv
作者: Hsu, Hsing-Wen
許馨文
關鍵字: Ampelopsis brevipedunculata (Maxim.) Trautv
大本山葡萄
enterovirus 71
coxsackievirus B3
prodelphinidin B3-3-O-gallate
procyanidin B2-3-O-gallate
腸病毒71型
克沙奇病毒B3
prodelphinidin B3-3-O-gallate
procyanidin B2-3-O-gallate
出版社: 食品暨應用生物科技學系所
引用: 行政院衛生署中醫藥委員會。Procyanidin B-2,3,3''-di-O-gallate。中藥對照用指標成分物理化學資料彙編。89頁。2002。行政院衛生署中醫藥委員會。台北。 行政院衛生署食品藥物管理局。http://food.doh.gov.tw/FoodNew/info/Material.aspx。 李岳鴻。中草藥水萃取物毒性、血清生化及免疫相關指標之評估。2005。中興大學,碩士,台中。 林青怡。探討七種中草藥對腸道生理功能之影響。2004。中興大學,碩士,台中。 林俊良。台灣肝炎民間藥材之開發第二報山葡萄。1987。台北醫學院,碩士,台北。 徐志宏、劉星瑎、徐光昇。蛇葡萄跟化學成分的研究。中國中藥雜誌。1995; 20: 484-5。 徐美如。中草藥水萃取物抗氧化及誘導肝癌細胞凋亡之研究。2005。中興大學,碩士,台中。 梁佳玟、賴怡君、朱燕華。中草藥對於促發炎細胞激素生成之影響。中華醫誌。 2004; 15: 293-304。 湯芳萍、吳翠芳。山葡萄化學成分的研究。湖南中醫學院學報。2003; 23: 21-22。 黃玲惠。細胞培養技術訓練課程。2000。教育部生物技術改良課程。 劉怡玫。中草藥水萃取物制突變、抗制突變及抗發炎作用之研究。2005。中興大學,碩士,台中。 劉武哲。醫用病毒學及其研究方法 (修訂版)。117頁。2003。藝軒圖書出版社。台北。 謝文聰、譚思濰、陳介甫、蔡輝彥。山葡萄抽取物及其活性成分之鎮痛抗炎作用研究。中國醫藥學院雜誌。1998; 7: 81-87。 Abe Y, Shoju T, Kawahara N, Kamakura H, Kanda T, Goda Y, Ozeki, Y. Structural characterization of a procyanidin tetramer and pentamer from the apple by low-temperature NMR analysis. Tetrahedron let. 2008; 49: 6413-6418. AbuBakar S, Sam IC, Yusof J, Lim MK, Misbah S, MatRahim N, Hooi PS. Enterovirus 71 outbreak, Brunei. Emerg Infect Dis. 2009; 15: 79-82. Arita M, Wakita T, Shimizu H. Characterization of pharmacologically active compounds that inhibit poliovirus and enterovirus 71 infection. J Gen Viro. 2008; 89: 2518-2530. Artym J, Zimecki M, Kruzel ML. Effects of lactoferrin on IL-6 production by peritoneal and alveolar cells in cydophosphamide-treated mice. J Chemother. 2004; 16: 187-192. Beig FK, Malik A, Rizvi M, Acharya D, Khare S. Etiology and clinico-epidemiological profile of acute viral encephalitis in children of western Uttar Pradesh, India. Int J Infect Dis. 2010; 14: e141-146. Bergelson JM, Krithivas A, Celi L, Droguett G, Horwitz MS, Wickham T, Crowell RL, Finberg RW. The murine CAR homolog is a receptor for Coxsackie B virus and adenovirus. J Virol. 1998; 72: 415-419. Buenz EJ, Howe CL. Picornaviruses and cell death. Trends Microbiol. 2006; 14: 28-36. Callemien D, Guyot S, Collin S. Use of thiolysis hyphenated to RP-HPLC-ESI(-)-MS/MS for the analysis of flavaniods in fresh lager beers. Food Chem. 2008; 110: 1012-1018. Cardoso CL, Silva DHS, Castro-Gamboa I, Bolzan VS. New biflavonoid and other flavonoids from the leave of Chimarrhis turbinate and their antioxidant activities. J Braz Chem Soc. 2005; 16: 1353-1359. Carr D, Campbell IL. Transgenic expression of interleukin-6 in the central nervous system confers protection against acute herpes simplex virus type-1 infection. J Neurovirol. 1999; 5: 449-457. Center for Disease Control; Executive Yuan, Department of Health. CDC Annual Report 2006. Executive Yuan, 2009; Taipei, Taiwan. Chan KP, Goh KT, Chong CY, Teo ES, Lau G, Ling AE. Epidemic hand, foot and mouth disease caused by human enterovirus 71, Singapore. Emerg Infect Dis. 2003; 9: 78-85. Chan LG, Parashar UD, Lye MS, Ong FGL, Zaki SR, Alexander JP, Ho KK, Han LL, Pallansch MA, Suleiman AB, Jegathesan M. Deaths of children during an outbreak of hand, food, and mouth disease in Sarawak, Malaysia: clinical and pathological characteristics of the disease. Clin Infect Dis. 2000; 31: 678-683. Chan YF, Sam IC, AbuBakar S. Phylogenetic designation of enterovirus 71 genotypes and subgenotypes using complete genome sequences. Infect Genet Evol. 2010; 10: 404-412. Changa LY. Enterovirus 71 in Taiwan. Pediatr Neonatol. 2008; 49: 103-112. Chang LY, Hsiung CA, Lu CY, Lin TY, Huang FY, Lai YH, Chiang YP, Chiang BL, Lee CY, Huang LM. Status of cellular rather than humoral immunity is correlated with clinical outcome of enterovirus 71. Pediatric Research 2006; 60: 466-471. Changb JS, Wang KC, Chiang LC. Sheng-Ma-Ge-Gen-Tang inhibited enterovirus 71 infection in human foreskin fibroblast cell line. J Ethnopharcol. 2008; 119: 104-108. Chen HL, Huang JY, Chu TW, Tsai TC, Hung CM, Lin CC, Liu FC, Wang LC, Chen YJ, Lin MF, Chen CM. Expression of VP1 protein in the milk of transgenic mice: a potential oral vaccine protects against enterovirus 71 infection. Vaccine 2008; 26: 2882-2889. Chen CS, Yao YC, Lin SC, Lee YP, Wang YF, Wang JR, Liu CC, Lei HY, Yu CK. Retrograde axonal transport: a major transmission route of enterovirus 71 in mice. J Virol. 2007; 81: 8996-9003. Chena HF, Chang MH, Chiang BL, Jeng ST. Oral immunization of mice using transgenic tomato fruit expressing VP1 protein from enterovirus 71. Vaccine 2006; 24: 2944-2951. Chenb LC, Yeh TM. Enterovuris 71 infection of human immune cells induces the production of proinflammatory cytokines. J Biomed Lab Sci. 2009; 21: 82-89. Chen TC, Chang HY, Lin PF, Chern JH, Hsu JTA, Chang CY, Shin SR. Novel antiviral agent DTriP-22 targets RNA-dependent RNA polymerase of enterovirus 71. Antimicrob Agents Chemother. 2009; 53: 2740-2747. Chern JH, Shia KS, Hsu TA, Tai CL, Lee CC, Lee YC, Chang CS, Tseng SN, Shih SR. Design, synthesis, and structure-activity relationships of pyrazolo[3,4-d]pyrimidines: a novel class of potent enterovirus inhibitors. Bioorg Med Chem Lett. 2004; 14: 2519-2525. Cheung PK, Yuan J, Zhang HM, Chau D, Yanagawa B, Suarez A, McManus B, Yang D. Specific interactions of mouse organ proteins with the 5’untraslated region of coxsackievirus B3: potential determinant of viral tissue tropism. J Med Virol. 2005; 77: 414-424. Chiang LC, Ng LT, Cheng PW, Chiang W, Lin CC. Antiviral activities of extracts and selected pure constituents of Ocimum Basilicum. Clin Exp Pharmacol Physiol. 2005; 32: 811-816. Choi HJ, Lim CH, Song JH, Baek SH, Kwon DH. Antiviral activity of raoulic acid from Raoulia australis against Picornaviruses. Phytomedicine 2009; 16: 35-39. Choi HJ, Song JH, Ahn YJ, Kwon DH. Inhibitory effect on replication of enterovirus 71 of herb methanol extract. J Appl Biol Chem. 2008; 51: 123-127. Choi HJ, Song JH, Park KS, Baek SH. In vitro anti-enterovirus 71 activity of gallic acid from Woodfordia fruticosa flowers. Lett Appl Microbiol. 2010; 50: 438-440. Chou SC, Kaur M, Agarwal R, Agarwal C. Influence of gallate esterification on the activity of procyanidin B2 in androgrn-dependent human prostate carcinoma LNCaP cels. Pharm Res. 2010; 27: 619-627. Chow LH, Beisel KW, McManus BM. Enteroviral infection of mice with severe combined immunodeficiency. Evidence for direct viral pathogenesis of myocardial injury. Lab Invest. 1992; 66: 24-31. Chu PY, Ke GM, Chen YS, Lu PL, Chen HL, Lee MS, Chen BC, Huang TS, Li YC, Chou LC, Wang SY, Lin KH. Molecular epidemiology of Coxsackievirus B3. Infect Genet Evol. 2010; 10: 777-784. Chumakov M, Voroshilova M, Shindarov L, Lavrova I, Gracheva L, Koroleva G, Vasilenko S, Brodvarova I, Nikolova M, Gyurova S, Gacheva M, Mitov G, Ninov N, Tsylka E, Robinson I, Frolova M, Bashkirtsev V, Martiyanova L, Rodin V. Enterovirus 71 isolated from cases of epidemic poliomyelitis-like disease in Bulgaria. Arch. Virol 1979; 60: 329-340. Chung YC, Ho MS, Wu JC, Chen WJ, Huang JH, Chou ST, Hu YC. Immunization with virus-like particles of enterovirus 71 elicits potent immune responses and protects mice against lethal challenge. Vaccine 2008; 26: 1855-1862. Coeder R, Mullen W, Khan NQ, Marks SC, Wood EG, Carrier MJ, Crozier A. Red wine procyanidins and vascular health. Nature 2006; 444: 556. Contreras-Dominguez M, Guyot S, Marnet N, Petit JL, Perraud-Daime I, Roussos S, Augur C. Degradation of procyanidins by Aspergillus fumigatus: Identification of a novel aromatic ring cleavage product. Biochimie 2006; 88: 1899-1908. Dalldorf G, Sickles G. An unidentified, fitltrable agent isolated from the faces of children with paralysis. Science 1948; 108: 61-62. Dan M, Chantler JK. A genetically engineered attenuated Coxsackievirus B3 strain protects mice against lethal infection. J Virol. 2005; 79: 9285-9295. Davis AL, Cai Y, Davies AP, Lewis JR. 1H and 13C NMR assignments of some green tea polyphenols. Magn Reson Chem. 1996; 34: 887-890. Dawson RMC, Elliott DC, Elliott WH, Jones KM. Data for biochemical research. pH and Buffers. 1978. Clarendon Press, Oxoford, pp. 483-485. Dechkum N, Pangsawan Y, Jayavasu C, Saquanwongse S. Coxsackie B virus infection and myopericarditis in Thailand, 1987-1989. Southeast Asian J Trop Med Public Health. 1998; 29: 273-276. Deiana M, Dessi MA, Ke B, Liang YF, Higa T, Gilmour PS, Jen LS, Rahman I, Aruoma OI. The antioxidant cocktail effective microorganism X (EM-X) inhibits oxidant-induced interleukin-8 release and the peroxidation of phospholipids in virto. Biochem Biophys Res Commun. 2002; 296: 1148-1151. Dinarello CA. Immunological and inflammatory functions of the interleukin-1 family. Annu. Rev. Immunol. 2009; 27: 519-550. Doraisingham S. Isolation of enterovirus type 71 in Singapore. Virus information Exchange Newsletter for South-East Asia and the Western Pacific 1985; 2: 39. Eloff JN. Quantification the bioactivity of plant extracts during screening and bioassay guided fractionation. Phytomedicine 2004; 11: 370-371. Esfandiarei M, McManus BM. Molecular biology and pathogenesis of viral myocarditis. Annu Rev Pathol Mech Dis. 2008; 3: 127-155. Fairweather D, Rose NR. Inflammatory heart disease: a role for cytokines. Lupus 2005; 12: 646-651. Feghali CA, Wright TM. Cytokines in acute and chronic inflammation. Front Biosci. 1997; 2: 12-26. Fine AM. Oligomeric proanthocyanidin complexes: history, structure, and phytopharmaceutical applications. Altern Med Rev. 2000; 5: 144-151. Foo LY, Lu Y, Molan AL, Woodfield DR, McNabb WC. The phenols and prodephinidin of white clover flowers. Phytochemistry 2000; 54: 539-48. Forrest GI, Bendall DS. The distribution of polyphenols in the tea plant (Camella sinensis L.). Biochem J. 1969; 113: 741-755. Friedrich W, Eberhardt A, Galensa R. Investigation of proanthocyanidins by HPLC with electrospray ionization mass spectrometry. Eur Food Res Technol. 2000; 211: 56-64. Fu YC, Chi CS, Chiu YT, Hsu SL, Hwang B, Jan SL, Chen PY, Huang FL, Chang Y. Cardiac complications of enterovirus rhombencephalitis. Arch Dis Child. 2004; 89: 368-373. Fuleki T, Ricardo da Silva JM. Catechin and procyanidin composition of seeds from grape cultivats grown in Ontario. J Agri Food Chem. 1997; 45: 1156-1160. Garozzo A, Cutri CCC, Castro A, Tempera G, Guerrera F, Sarva MC, Geremia E. Anti-rhinovirus activity of 3-methylthio-5-aryl-4-isothiazolecarbonitrile derivatives. Antiviral Res. 2000; 45: 199-210. Gauntt CJ, Pallansch MA. Coxsackievirus B3 clinical isolates and murine myocarditis. Virus Res. 1996; 41: 89-99. Gawel R, Iland PG, Francis IL. Characterizing the astringency of red wine: a case study. Food Qual Pref. 2001; 12: 83-94. Gebhard JR, Perry CM, Harkins S, Lane T, Mena I, Asensio VC, Campbell LL, Whitton JL. Coxsackievirus B3-induced myocarditis: perforin exacerbates disease, but plays no detectable role in virus clearance. Am J of Pathol. 1998; 153: 417-428. Gebre-Mariam T, Neubert R, Schmidt PC, Wutzler P, Schmidtke M. Antiviral activities of some Ethiopian medicinal plants used for the treatment of dermatological disorders. J Ethnopharmacol. 2006; 104: 182-187. Genovese D, Conti C, Tomao P, Desideri N, Stein ML, Catone S, Fiore L. Effect of chloro-, cyano-, and amidino-substituted flavanoids on enterovirus infection in vitro. Antiviral Res. 1995; 27: 123-136. Godeny EK, Arizpe HM, Gauntt CJ. Characterization of the antibody response in vaccinated mice protected against coxsackievirus B3-induced myocarditis. Viral Immunol. 1987/1988; 1: 305-315. Hashimoto F, Nonaka G, Nishioka I. Tannins and related compounds LXXVII. Novel chalcan-flavan dimmers, assamicains A, B, and C, and a new Flavan-3-ol and proanthocyanidins from the fresh leaves of Camellia sinensis L. var. assamica kitamura. Chem Phar Bull. 1989; 37: 77-85. Hagiwara A, Tagaya I, Yoneyama T. Epidemic of hand, foot and mouth disease associated with enterovirus 71 infection. Intervirology 1978; 9: 60-63. Harrison DN, Gazina EV, Purcell DF, Anderson DA, Petrou S. Amiloride derivatives inhibit coxsackievirus B3 RNA replication. J Virol. 2008; 82: 1465-1473. Henke A, Chiang CS, Zell R, Stelzner A. Co-expression of interleukin-2 to increase the efficacy of DNA vaccine-mediated protection in coxsackie virus B3-infected mice. Antiviral Res. 2004; 64: 131-136. Henke A, Huber S, Stelzner A, Whitton L. The role of CD8+ T lymphocytes in coxsackievirus B3-induced myocarditis. J Virol. 1995; 69: 6720-6728. Henke A, Jarasch N, Martin U, Wegert J, Wildner A, Zell R, Wutzler P. Recombinant coxsackivirus vectors for prevention and therapy of virus-induced heart disease. Int J Med Microbiol. 2008; 298: 127-134. Henke A, Mohr C, Sprenger H, Graevner C, Stelzner A, Nain M, Gemsa D. Coxsackievirus B3-induced production of tumor necrosis factor-α, IL-1β, and IL-6 in human monocytes. J Immunol. 1992; 148: 2270-2277. Henke A, Wagner E, Whitton JL, Zell R, Stelzner A. Protection of mice against lethal Coxsackievirus B3 infection by using DNA immunization. J Virol. 1998; 72: 8327-8331. Henke A, Zell R, Stelzner A. DNA vaccine-mediated immune responses in coxsackie virus B3-infected mice. Antiviral Res. 2001; 49: 49-54. Henke H. Preparative gel chromatography on Sephadex LH-20. 1996. Huthig Verlag Heidelberg. Ho HY, Cheng ML, Weng SF, Leu YL, Chiu DTY. Antiviral effect of epigallocatechin gallate on enterovirus 71. J Agric Food Chem. 2009; 57: 6140-6147. Ho M, Chen ER, Hsu KH, Twu SJ, Chen KT, Tsai SF, Wang JR, Shih SR. An epidemic of enterovirus 71 infection in Taiwan. N Eng. J Med. 1999; 341: 929-935. Hofmann P, Schmidtke M, Stelzner A, ZGemsa D. Suppression of proinflammatory cytokines and induction of IL-10 in human monocytes after coxsackievirus B3 infection. J Med Virol. 2001; 64: 487-498. Honda T, Saitoh H, Masuko M, Katagiri-Abe T, Tominaga K, Kozakai I, Kobayashi K, Kumanishi T, Watanabe YG, Odani S, Kuwano R. The coxsackievirus-adenovirus receptor protein as a cell adhesion molecule in the developing mouse brain. Brain Res Mol Brain Res. 2000; 77: 19-28. Hsieh WT, Tan TW, Chen CF, Tsai HY. Studies of anti-inflammatory and analgesic effects of Ampelopsis brevipedunculata. Chin Med Coll J. 1998; 7: 81-87. Hsiung GD. Diagnostic virology: illustrated by light and electron microscopy. 1982; 3rd ed. Yale University Press. New Haven. Hu YC, Hsu JTA, Huang JH, Ho MS, Ho YC. Formation of enterovirus-like particle aggregates by recombinant baculoviruses co-expressing P1 and 3CD in insect cells. Biotechnol Lett. 2003; 25: 919-925. Huber S. Host Immune Responses to Coxsackie B3. Curr Top Microbiol Immunol. 2008; 323: 199-221. Huber S, Polgar J, Schultheiss P, Schwimmbeck P. Augmentation of pathogenesis of coxsackievirus B3 infections in mice by exogenous adiministraion of interleukin-1 and interleukin-2. J Virol. 1994; 68: 195-206. Hummer W, Schreier P. Analysis of Proanthocyanidins. Mol Nutr Food Res. 2008; 52: 1381-1398. Hung HC, Chen TC, Fang MY, Yen KJ, Shih SR, Hsu JTA, Tseng CP. Inhibition of enterovirus 71 replication and the viral 3D polymerase by aurintricarboxylic acid. J Antimicrob Chemother. 2010; 65: 676-683. Hunziker IP, Harkins S, Feuer R, Cornell CT, Whitton JL. Generation and analysis of an RNA vaccine that protects against coxsackievirus B3 challenge. Virology 2004; 330: 196-208. Karonen M, Loponen J, Ossipov V, Pihlaja K. Analysis of procyanidins in pine bark with reversed-phase and normal-phase high-performance liquid chromatography-electrospray ionization mass spectrometry. Anal Chem Acta. 2004; 522: 105-112. Kato T, Yamane F, Morita Y. The flavonol glycosides from Ampelopsis brevipedunculata Trautv. Shoyakugaku Zasshi 1989; 43: 266. Kato T, Yamane F, Morita Y. The flavonol glycosides and other constituents from the leaves of Ampelopsis brevipedunculata Trautv. Shoyakugaku Zasshi 1990; 44: 138. Kennett ML, Birch CJ, Lewis FA, Yung AP, Locarnini SA, Gust ID. Enterovirus type 71 infection in Melbourne. Bull. World Health Organ 1974; 51: 609-615. Khallouki F, Haubner R, Hull WE, Erben G, Spiegelhalder B, Bartsch H, Owen RW. Isolation, purification and identification of ellagic acid derivatives, catechins, and procyanidins from the root bark of Anisophyllea dichostyla R. Br. Food Chem Toxicol. 2007; 45: 472-485. Khetsuriani N, LaMonte-Fowlkes A, Oberste S, Pallansch M. Enterovirus Surveillance-United States, 1970-2005. Surveillance Summaries 2006; 55: 1-20. Kiatgrajai P, Wellons JD, Gollob L, White JD. Kinetics of epimerization of (+)-catechin and its rearrangement to catechin acid. J Org Chem. 1982; 47: 2910-2912. Kim JY, Jeon ES, Lim BK, Kim SM, Chung SK, Kim JM, Park SI, Jo I, Nam JH. Immunogenicity of a DNA vaccine for coxsackievirus B3 in mice: protective effects of capsid proteins against viral challeng. Vaccine 2005; 23: 1672-1679. Kim KS, Hufnagel G, Chapman NM, Tracy S. The group B coxsackieviruses and myocarditis. Rev Med Virol. 2001; 11: 355-368. Kishimoto C, Kuroki Y, Hiraoka Y, Ochiai H, Kurokama M, Sasayama S. Cytokine and murine coxsackievirus B3 myocarditis. Interleukin-2 suppressed myocarditis in the acute stage but enhance the condition in the subsequent stage. Circulation 1994; 89: 2836-2842. Kohler N, Wray V, Winterhalter P. Preparative isolation of procyanidins from grape seed extracts by high-speed counter-current chromatography. J Chromatogr A. 2008; 1177: 114-125. Komatsu Y, Suematsu S, Hisanobu Y, Saigo H, Matsuda R, Hara K. Effects of pH and temperature on reaction kinetics of catechins in green tea infusion. Biosci Biotech Biochem. 1993; 57: 907-910. Kondo K, Kurihara M, Fukuhara K, Tanaka T, Suzuki T, Miyata N, Toyoda M. Conversion of procyanidin B-type (catechin dimer) to A type: evidence for abstraction of C-2 hydrogen in catechin during radical oxidation. Tetrahedron Lett. 2000; 41: 485-488. Kumar NS, Rajapaksha M. Separation of catechin constituents from five tea cultivars using high-speed counter-current chromatography. J Chromatogr A. 2005; 1083: 223-228. Kuo CJ, Shie JJ, Fang JM, Yen GR, Hsu JTA, Liu HG, Tseng SN, Chang SC, Lee CY, Shih SR, Liang PH. Design, synthesis, and evaluation of 3C protease inhibitors as anti-enterovirus 71 agents. Bioorg Med Chem. 2008; 16: 7388-7398. Kuo CJ, Shie JJ, Fang JM, Yen GR, Hsu JTA, Liu HG, Tseng SN, Chang SC, Lee CY, Shih SR, Liang PH. Design, synthesis, and evaluation of 3C protease inhibitors as anti-enterovirus 71 agents. Bioorg Med Chem. 2008; 16: 7388-7398. Kuo TM, Hu CP, Chen YL, Hong MH, Jeng KS, Liang CC, Chen ML, Chang C. HBV replication is significantly reduced by IL-6. J Biomed Sci. 2009; 16:41. Kuo YC, Sun CM, Tsai WJ, Ou JC, Chen WP, Lin CY. Blocking of cell proliferation, cytokines production and genes expression following administration of Chinese herbs in the human mesangial cells. Life Sci. 1999; 64: 2089-2099. Lai SK, Huang CC, Jiang CH, Tsai YT, Chang HL, Chuang JH. Surveillance for epidemic of enterovirus infections in Taiwan in 2008. Int J Infect Dis. 2010; 14 Supplement 1: e176-177. Leblanc RA, Pesnicak L, Cabral ES, Godleski M, Straus E. Lack of interleukin-6 enhances susceptibility to infection but does not alter latency or reactivation of herpes simplex virus type 1 in IL-6 knockout mice. J Virol. 1999; 73: 8145-8151. Lee H, Lin JY. Antimutagenic activity of extracts from anticancer drugs in Chinese medicine. Mutat Res. 1988; 204: 229-234. Lee ST, Ki CS, Lee NY. Molecular characterization of enteroviruses isolated from patients with aseptic meningitis in Korea, 2005. Arch Virol. 2007; 152: 963-970. Lee YP, Huang SW, Yu CK. Modification of the type I interferon and proinflammatory cytokine responses by enterovirus 71. Int J Infect Dis. 2010; 14: supplement 1 e463-464. Li ZH, Li CM, Ling P, Shen FH, Chen SH, Liu CC, Yu CK, Chen SH. Ribavirin reduces mortality in enterovirus 71-infected mice by decreasing viral replication. J Infect Dis. 2008; 197: 854-857. Lim T, Yuan J, Zhang JM, Sall A, Liu Z, Su Y, Yang D. Antisense DNA and RNA agents against picornaviruses. Front Biosci. 2008; 4707-4725. Lin CW, Wu CF, Hsiao NW, Chang CY, Li SW, Wan L, Lin YJ, Lin WY. Aloe-emodin is an interferon-inducing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71. Int J Antimicro Ag. 2008; 32: 355-359. Lin JY, Chen TC, Weng KF, Chang SC, Chen LL, Shih SR. Viral and host proteins involved in piconavirus life cycle. J Biomed Sci. 2009a; 16: 103. Lin TL. Chen KT, Wang ET, Li YS, Huang CW, Hsu CC, Wu HS. A serotype analysis of individual cases of severe enterovirus in Taiwan region, 1998-2006. Taiwan Epidem Bull. 2007; 23: 514-530. Lin TY, Chu C, Chiu CH. Lactoferrin inhibits enterovirus 71 infection of human embryonal rhabdomyosarcoma cell in vitro. J Infect Dis. 2002a; 186: 1161-1164. Lin TY, Liu YC, Jheng JR, Tsai HP. Jan JT, Wong WR, Horng JT. Anti-enterovirus 71 activity screening of Chinese herbs with anti-infection and inflammation activities. Am J Chin Med. 2009b; 37: 143-158. Lin YT, Chang LY, Huang YC, Hsu KH, Chiu CH, Yang KD. Different proinflammatory reactions in fatal and non-fatal enterovirus 71 infections: implications for early recognition and therapy. Acta Padiatr. 2002b; 91: 632-635. Lin TY, Hsia SH, Huang YC, Wu CT, Chang LY. Proinflammatory cytokine reactions in enterovirus 71 infections of the central nervous system. Clin Infect Dis. 2003; 36: 269-274. Lin YW, Wang SW, Tung YY, Chen SH. Enterovirus 71 infection of human dendritic cells. Exp Biol Med. 2009c; 234: 1164-1173. Liu CC, Tseng SW, Wang SM, Wang JR, Su IJ. An outbreak of enterovirus 71 infection in Taiwan, 1998: epidemiologic and clinical manifestation. J Clin Virol. 2000; 17: 23-30. Liu JN, Wang W, Duo JY, Hao Y, Ma CM, Li WB, Lin SZ, Gao XZ, Liu XL, Xu YF, Xu WB, Qin C, Zhang LF. Combined peptides of human enterovirus 71 protect against virus infection in mice. Vaccine 2010; 28: 7444-7451. Makarov VA, Riabova OB, Granik VG, Wutzler P, Schmidtke M. Novel [(biphenyloxy)propyl]isoxazole derivatives for inhibition of human rhinovirus 2 and coxsackievirus B3 replication. J Antimicrob Chemother. 2005; 55: 483-488. Mavrouli MD, Spanakis N, Levidiotou S, Politi C, Alexiou S, Tseliou P, Hatzitaki M, Foundouli K, Tsakris A, Legakis NJ, Routsias JG. Serologic Prevalence of Coxsackievirus group B in Greece. Viral Immunol. 2007; 20: 11-18. Melnick JL. Enteroviruses. In: Fields BN, et al. (Eds.). Fields Virology, 3rd ed. Lippincott-Raven Press, Philadelphia, USA. 1996: 655-658. M’hadheb-Gharbi MB, Gharbi J, Paulous S, Brocard M, Komaromva A, Aouni M, Kean KM. Effects of the Sabin-like mutations in domain V of the internal ribosome entry segment on translational efficiency of the Coxsackievirus B3. Mol Gen Genomics. 2006; 276: 402-412. Mikami S, Kawashima S, Kanazawa K, Hirata KI, Kadayama Y, Hotta H, Hayashi Y, Ito H, Yokoyama M. Expression of nitric oxide synthase in a murine model of viral myocarditis induce by coxsackievirus B3. Biochem Biophys Res Commun. 1996; 220: 983-989. Mistchenko AS, Viegas M, Latta MPD, Barrero PR. Molecular and epidemiologic analysis of enterovirus B neurological infection in Argentine children. J Clin Viol. 2006; 37: 293-299. Monagas M, Gomez-Cordoves C, Bartolome B, Laureano O, Ricardo da Silva JM. Monomeric, oligomeric, and polymerica flavan-3-ol composition of wines and grapes from Vitis vinifera L. Cv. Graciano, Tempranillo, and Cabernet Sauvignon. J Food Chem Agric. 2003; 51: 6475-6481. Moorman JP, Fitzgerald SM, Prayther DC, Lee SA, Chi DS, Krishnaswamy G. Induction of p38-and gC1qR-dependent IL-8 expression in pulmonary fibroblasts by soluble hepatitis C core protein. Respir Res. 2005; 6: 105. Nagy G, Takatsy S, Kukan E, Mihaly I, Domok I. Virological diagnosis of enterovirus 71 infection: Experiences gained during an epidemic of acute CNS disease in Hungary in 1978. Arch. Virol. 1982; 71: 217-227. Nairn C, Clement GB. A study of Enterovirus isolations in Glasgow from 1977 to 1997. J Med Virol. 1999; 58: 304-312. Ooi MH, Wong SC, Lewthwaite P, Cardosa MJ, Solomon T. Clinical features, diagnosis, and management of enteorvirus 71. Lancet Neurol. 2010; 9: 1097-1105. Palma AMD, Heggermont W, Lanke K, Coutard B, Bergmann M, Monforte AM, Canard B, Clercq ED, Chimirri A, Puesringer G. Rohayem J. Kuppeveld F, Neyts J. The thiazolobenzimidazole TBZE-029 inhibits enterovirus replication by targeting a short region immediately donstream from motif C in the nonstructural protein 2C. J Virol. 2008; 82: 4720-4730. Park HH, Kim DS, Cho YJ, Kim YJ, Jeong SY, Lee SM, Cho SJ, Yun CW, Jo I, Nam JH. Attenuation of coxsackievirus B3 by VP2 mutation and its application as a vaccine against virus-induced myocarditis and pancreatitis. Vaccine 2009; 27: 1974-1983. Poffenberger MC, Straka N, Warry NE, Fang D, Shanina I, Horwitz S. Lack of IL-6 during coxsackievirus infection heightens the early immune response resulting in increased severity of chronic autoimmune myocarditis. PLoS One 2009; 4: e6027. Rabenau HF, Richter M, Doerr HW. Hand food and mouth disease: seroprevalence of coxsackie A16 and enterovirus 71 in Germany. Med Microbiol Immunol. 2010; 199: 45-51. Ricardo da Silva JM, Darmon N, Fernandex Y, Mitjavila S. Oxygen free radical scavenger capacity in aqueous models of different procyanidins from grape seed. Oxygen free radical scavenger capacity in aqueous models of different procyanidins from grape seeds. J Agric Food Chem. 1991; 39: 1549-1552. Rogez-Kreuz C, Maneglier B, Martin M, Dereuddre-Bosquet N, Martal J, Dormont D, Clayette P. Involvement of IL-6 in the anti-human immunodeficiency virus activity of IFN-τ in human macrophage. Int Immuno. 2005; 17: 1047-1057. Rossouw E, Tsilimigras CW, Schoub BD. Molecular epidemiology of a coxsackievirus B3 outbreak. J Med Virol. 1991; 34: 165-171. Rueckert RR. Picornaviridae. In: Fields BN, et al. (Eds.). Fields Virology, 3rd ed. Lippincott-Raven Press, Philadelphia, USA.1996: 610-626. Saito A, Mizushina Y, Ikawa H, Yoshida H, Doi Y, Tanaka A, Nakajima N. Systematic synthesis of galloyl-substituted procyanidin B1 and B2, and their ability of DPPH radical scavenging activity and inhibitory activity of DNA polymerases. Bioorg Med Chem. 2005; 13: 2759-2771. Samuda GM, Chang WK, Yeung CY. Tang PS. Monoplegia caused by enterovirus 71: an outbreak in Hong Kong. Pediatr Infect Dis J. 1987; 6: 206-208. Schmidt NJ, Lennette EH, Ho HH. An apparently new enterovirus isolated from patients with disease of central nervous system. J Infect Dis. 1974; 129: 304-309. Schuffenecker I, Mirand A, Antona D, Henquell C, Chomel JJ, Archimbaud C, Billaud G, Peigue-Lafeuille H, Lina B, Bailly JL. Epidemiology of human enterovirus 71 infections in France, 2000-2009. J Clin Virol. 2011; 50: 50-56. Sehgal PB. Interleukin-6: Molecular Pathophysiology. J Invest Dermatol. 1990; 94: 2-6. Shi J, Nawaz H, Pohorly J, Mittal G, Kakuda Y, Jiang Y. Extration of polyphenolics from plant material for functional foods-engineering and technology. Food Rev Int. 2005; 21: 139-166. Shi L, Xiong H, He J, Deng H, Li Q, Zhong Q, Hou W, Cheng L, Xiao H, Yang Z. Antiviral activity of arbidol against influenza A virus, respiratory syncytial virus, rhinovirus, coxsackie virus and adenovirus in vitro and in vivo. Arch Virol. 2007; 152: 1447-1455. Shia KS, Li WT, Chang CM, Hsu MC, Chern JH, Leong MK, Tseng SN, Lee CC, Lee YC, Chen SJ, Peng KC, Tseng HY, Chang YL, Tai CL, Shih SR. Design, synthesis, and structure-activity relationship of pyridyl imidazolidinones: a novel class of potent and selective human enterovirus 71 inhibtor. J Med Chem. 2002; 45: 1644-1655. Shih SR, Tsai KT, Li YS, Chueh CC, Chan EC. Inhibition of enterovirus 71-induced apoptosis by allophycocyanin isolated from a blue-green alga Spirulina platensis. J Med Virol. 2003; 70: 119-125. Shih SR, Tsai MC, Tseng SN, Won KF, Shia KS, Li WT, Chern JH, Chen GW, Lee CC, Lee YC, Peng KC, Chao YS. Mutation in enterovirus 71 capsid protein VP1 confers resistance to the inhibitory effect of pyridyl imidazolidinone. Antimicrob Agents Chemother. 2004; 48: 3523-3529. Shoji T, Mutsuga M, Nakamura T, Kanda T, Akiyama H, Goda Y. Isolation and structural elucidation of some procyanidins from apple by low-temperature nuclear magnetic resonance. J Agric Food Chem. 2003; 51: 3806-3813. Solomon T, Lewthwaite P, Perera D, Cardosa MJ, McMinn P, Ooi MH. Virology, epidemiology, pathogenesis, and control of enterovirus 71. Lancet Infect Dis. 2010; 10: 778-790. Song X, Liu Z, Wang H, Xin Y, Wang X, Chen J, Shi Y, Zhang C, Hui R. QiHong prevents death in Coxsackievirus B3-induced murine myocarditis through inhibition of virus attachment and penetration. Exp Biol Med. 2007; 232: 1441-1448. Spanakis N, Manolis EN, Tsakris A, Tsiodras S, Panagiotopoulos T, Saroglou G, Legakis NJ. Coxsackievirus B3 sequences in the myocardium of fatal cases in a cluster of acute myocarditis in Greece. J Clin Pathol. 2005; 58: 357-360. Spranger I, Sun B, Mateus AM, Freitas V, Ricardo-da-Silva JM. Chemical characterization and antioxidant activities of oligomeric and polymeric procyanidin fractions from grape seeds. Food Chem. 2008; 519-532. Srivastava S, Khanna N, Saxena SK, Singh A, Mathur A, Dhole TN. Degradation of Japanese encephalitis virus by neutrophils. Int J Exp Pathol. 1999; 80: 17-24 Su M, Li Y, Leung KT, Cen Y, Li T, Chen R, Ooi VEC. Antiviral activity and constituent of Ardisia chinensis Benth against Coxsackie B3 virus. Phytother Res. 2006; 20: 634-639. Sun X, Guan YX, Luo XL, Yu YA, Gao HZ. Observation of the efficacy of Ampelopsis brevipedunculata Trautv. in the treatment of herpes zoster. J Tradit Chin Med. 1986; 6: 17-18. Takano F, Takata T, Yoshihara A, Nakamura Y, Arima Y, Ohta T. Aqueous extract of peanut skin and its main constituent procyanidin A1 suppress serum IgE and IgG1 level in mice-immunized with ovalbumin. Biol Pharm Bull. 2007; 30: 922-927. Tait S, Salvati AL, Desideri N, Fiore L. Antiviral activity of substituted homoisoflavonoids on enterovirus. Antiviral Res. 2006; 72: 252-255. Tarascou I, Barathieu K, Simon C, Ducasse MA, Andre Y, Fouquet E, Dofourc EJ, de Freitas V, Laguerre M, Pianet I. A 3D structural conformational study of procyanidin dimers in water and hydro-alcoholic media as viewed by NMR and molecular modeling. Magn Reson Chem. 2006; 44: 868-880. Tung WH, Hsieh HL, Yang CM. Enterovirus 71 induces COX-2 expression via MAPKs, NF-κB, and AP-1 in SK-N-SH cells: Role of PGE2 in viral replication. Cell Signal. 2010; 22: 234-246. Vallet S, Legrand-Quillien MC, Dailland T, Podeur G, Gouriou S, Schuffenecker I, Payan C, Marcorelles P. Fetal case of enterovirus 71 infection, France, 2007. Emerg Infect Diseases. 2009; 15: 1837-1840. Van der Sanden SMG, Koopmans MPG, Verduyn-Lunel F, Van der Avoor H, Galama JMD, Epidemiology of enterovirus 71 in the Netherlands, 1963-2007: change of dominant subgenogroup in time. J Clin Virol. 2009; 44: S10. Wanga H, Ding Y, Zhou J, Sun X, Wang S. The in vitro and in vivo antiviral effects of salidroside from Rhodiola rosea L. against coxsackievirus B3. Phytomedicine 2009; 16: 146-155. Wang SM, Lei HY, Huang KJ, Wu JM, Wang JR, Yu CK, Su IJ, Liu CC. Pathogenesis of enterovirus 71 brainstem encephalitis in pediatric patients: roles of cytokines and cellular immune activation in patients with pulmonary. J Infect Dis. 2003; 188: 564-570. Wang SM, Lei HY, Huang MC, Su LY, Lin HC, Yu CK, Wang JL, Liu CC. Modulation of cytokine production by intravenous immunoglobulin in patients with enterovirus 71-associated brainstem encephalitis. J Clin Virol. 2006; 37: 47-52. Wang YC, Huang TL. Screening of anti-Helicobacter pylori herbs deriving from Taiwanese folk medicinal plants. FEMS Immunol Med Mirobiol. 2005; 43: 295-300. Wang YF, Chou CT, Lei HY, Liu CC, Wang SM, Yan JJ, Su IJ, Wang JR, Yeh TM, Chen SH, Yu CK. A mouse-adapted enterovirus 71 strain causes neurological disease in mice after oral infection. J Virol. 2004; 78: 7916-7924. Wangb YF, Wang XY, Ren Z, Qian CW, Li YC, Kaio K, Wang QD, Zhang Y, Zheng LY, Jiang JH, Yang CR, Liu Q, Zhang YJ, Wang YF. Phyllaemblicin B inhibits coxsackie virus B3 induced apoptosis and myocarditis. Antiviral Res. 2009; 84: 150-158. Weng TY, Chen LC, Shyu HW, Chen SH, Wang JR, Yu CK, Lei HY, Yeh TM. Lactoferrin inhibits enterovirus 71 infection by biding to VP1 protein and host cells. Antiviral Res. 2005; 67: 31-37. Wu BW, Pan TL, Leu YL, Chang YK, Tai PJ, Lin KH, Horng JT. Antiviral effects of Salvia miltiorrhiza (Danshen) against enterovirus 71. Am J Chin Med. 2007; 35: 153-168. Wu CN, Lin YC, Fann C, Liao NS, Shih SR, Ho MS. Protection against lethal enterovirus 71 infection in newborn mice by passive immunization with subunit VP1 vaccines and inactivated virus. Vaccine 2002a; 20: 895-904. Wu JM, Wang JN, Tsai YC, Liu CC, Huang CC, Chen YJ, Yeh TF. Cardiopulmonary manifestations of fulminant enteroviruss 71 infection. Pediatics 2002b; 109: e26. Wu KX, Ng MML, Chu JJH. Developments towards antiviral therapies against enterovirus 71. Drug Discov Today. 2010a; 15: 1041-1051. Wu MJ, Yen JH, Wang L, Weng CY. Antioxidant activity of Porcelainberry (Ampelopsis brevipedunculata (Maxim) Trautv.). Am J Chin Med. 2004; 32: 681-693.
摘要: 本研究選用58種中草藥,以95%乙醇萃取,進行中和試驗,篩選具有抗EV71活性的中草藥。以時間點分析探討萃取物抗病毒機制,並測定萃取物對宿主細胞感染EV71免疫調節之影響。 選用的58種中草藥中,有6種萃取物 (大本山葡萄、台灣山豆根、風不動、螺厴草、野牡丹及雞血藤) 對所有測試之EV71皆有抑制活性。其中以大本山葡萄之平均半抑制濃度 (50% inhibitory concentrations, IC50) 最低為32.20 μg/ ml。進行進一步研究發現,大本山葡萄不同溶劑萃取物中,丙酮萃取物對EV71及克沙奇B3 (coxsackievirus, CVB3) 皆有良好的抗病毒活性,平均IC50值分別為26.11及45.83 μg/ ml,分別為正控制組 (ribavirin) 的4及36 %。萃取物經酸處理會降低其抗EV71及CVB3活性,經pH 2處理後對病毒之抑制率為原萃取物之50 ~ 70 %。大本山葡萄丙酮萃取物對EV71之抗病毒機制主要為預防病毒感染、使病毒失活及抑制病毒的早期複製,而對CVB3主要為使病毒失活及抑制病毒複製。萃取物會誘導已感染EV71之宿主細胞顯著地增加interleukin-6 (IL-6) 及interleukin-1β (IL-1β),而降低interleukin-8 (IL-8) 之生成量,綜合大本山葡萄丙酮萃取物抗EV71活性及免疫調節之結果,IL-6之增加與抗腸病毒71型有很高的關聯性,且IL-8之降低主要原因可能來自於大本山葡萄之抗氧化活性。 大本山葡萄丙酮萃取物經amberlite XAD-7、sephadex LH-20及半製備型C18-HPLC管柱層析,得到6個活性區分,其中區分E-8-II及E-8-IV對EV71及CVB3皆有良好的抑制活性,此兩區分經核磁共振光譜儀、質譜儀、紅外線光譜儀及紫外線光譜儀分析之結果,鑑定為原花青素化合物,分別為prodelphinidin B3-3-O-gallate及procyanidin B2-3-O-gallate。本研究首次揭露出此二化合物之抗EV71及CVB3活性,IC50分別為1.14 ± 0.09 ~ 153.58 ± 12.87 μg/ml (prodelphinidin B3-3-O-gallate) 及0.76 ± 0.09 ~ 71.16 ± 1.83 μg/ml (procyanidin B2-3-O-gallate)。Procyanidin B2-3-O-gallate之抗腸病毒活性較佳,且含量較高 (5.67 mg/ g粗萃物),為大本山葡萄之主要抗成病毒之活性成分。時間點分析之結果顯示出procyanidin B2-3-O-gallate對EV71及CVB3之抗病毒機制包括預防病毒感染、使病毒失活及抑制病毒的複製。
In this study, 58 selected meicinal plants were extracted with 95% ethanol and screened for their anti-EV71 activity with neutralization method. We investigated the antiviral modes of the extract with the time of addition assay and the effect of the extract on immune modulation in the EV71 infected host cell. Among 58 screened medicinal plants, 6 extracts inhibited all test EV71 strains including Ampelopsis brevipedunculata (Maxim.) Trautv (ABT), Euchresta formosana (Hayata) Ohwi, Ficus pumila L, Lemmaphyllum microphyllum Presl, Melastoma candidum D. Don and Spatholobi Caulis. The loweset average 50% inhibitory concentration (IC50) was observed in ABT extract of 32.20 μg/ ml. Among different solvent extracts of ABT, the acetone extract showed good antiviral effect on EV71 and coxsackievirus B3 (CVB3). The average IC50 of acetone extract against EV71 and CVB3 were 26.11 and 45.83 μg/ ml, respectively, 4 and 6% of that of the positive control (ribavirin). Acidic pH treatments decreased anti-EV71 activity of the extract. The inhibition rate of the pH 2 treated extract decreased to 50 ~ 70% of that of the original extract. The anti-EV71 modes of the ABT extract included viral infection prevention, viral inactivation and anti-viral early replication. The main anti-CVB3 modes of the ABT extract were viral inactivation and anti-viral replication. The ABT extract significantly increased the interlukin-6 (IL-6) and IL-1β and decreased the IL-8 levels in the EV71-infected cells. In summary of resusts for the anti-EV71 activity and immunomodulatory effect of ABT, high amounts of IL-6 induction in the infected-cells had high correlation with the anti-viral replication of the ABT extract and the decreasing IL-8 level was mostly contributed by the antioxidant activity of ABT. The ABT acetone extract was subjected to amberlite XAD-7, sephadex LH-20 and semi-pre C18-HPLC column chromatography to obtain six active fractions. Among them, fraction E-8-II and E-8-IV exhibited good anti-EV71 and anti-CVB3 activity. From the spectra of H-NMR, C-NMR, ESI-MS, FTIR and UV for the fractions E-8-II and E-8-IV, they were identified as prodelphinidin B3-3-O-gallate and procyanidin B2-3-O-gallate, respectivley, which had been firstly reported their anti-EV71 and anti-CVB3 activitly with the IC50 of 1.14 ± 0.09 ~ 153.58 ± 12.87 μg/ml (prodelphinidin B3-3-O-gallat) and 0.76 ± 0.09 ~ 71.16 ± 1.83 μg/ml (procyanidin B2-3-O-gallate), respectively. Procyanidin B2-3-O-gallate shows great antienterovirus effect and had higher content of the extract (5.67 mg / g crude extract), which was the main anti-EV71 compound of the ABT acetone extract. From the result of the time of addition assay, the anti-EV71 and anti-CVB3 modes of procyanidin B2-3-O-gallate included viral infection prevention, viral inactivation and anti-viral replication.
URI: http://hdl.handle.net/11455/51932
其他識別: U0005-0212201116441600
文章連結: http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-0212201116441600
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