Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/52016
標題: 不同研磨條件對甘草及黃柏有效成分提取及定量分析之影響
Effects of milling conditions on the extraction and analysis of Radix Glycyrrhizae Uralensis and Cortex Phellodendri extract
作者: 陳雋翰
Chen, Chun-Han
關鍵字: 中草藥
Chinese herbal materials
萃取物
微化處理
甘草
黃柏
extract
micronization
Radix Glycyrrhizae Uralensis
Cortex Phellodendri
出版社: 食品暨應用生物科技學系所
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摘要: 中草藥萃取物的製備對於中草藥之應用及成分分析,皆為很重要的環節。本研究選用甘草及黃柏兩種常用的中草藥素材,藉由不同方式的微化破碎處理,以生藥材做比對,探討不同研磨程度對於中草藥指標成分提取及定量之影響。 研究結果顯示,透過球磨處理的微化方式能有效降低甘草及黃柏粉末粒徑至3.4-3.8 um,同時能夠達到省時、耗能低及高產率的萃取量效果。有關活性物質萃取效果方面,甘草經過微化處理能大幅(p < 0.05)提升其甘草酸的萃取效果,並且在常溫(25°C)、短時間(15 min)的條件下即有生藥材高溫(100°C)、長時間(120 min)相當的產量。微化處理對黃柏中小檗鹼的萃取效果在高溫的條件時,能在較短的萃取時間(15-30 min)內達到相當於生藥材組長時間的萃取量。 整體而言,實驗結果證實適當的微化處理能提升中草藥萃取率和活性物質提取,且具有省時、耗能低及高產率的效果。本研究結果期望能為中草藥的微化技術和萃取方式提供參考,改善中草藥萃取物製備的研究與應用。
Preparation of Chinese herbal extract is the crucial step in the analysis of herbal and botanical industry. The present study was to investigate the effects of milling conditions on the extraction yield and analysis of both the Radix Glycyrrhizae Uralensis and Cortex Phellodendri materials. The results demonstrated that ball-milling could effectively (p < 0.05) pulverize the Glycyrrhizae and Phellodendron samples and reduce their particle sizes to 3.4-3.8 um. Meanwhile, a higher extraction yield could be achieved with micronized samples in a relatively shorter time and hence to reduce the energy consumption during extraction and drying. With respect to the extraction of bioactive compounds, micronization treatment significantly (p < 0.05) increased the extraction yield of glycyrrhizic acid from the Glycyrrhizae samples. The extraction yields of the micronized Glycyrrhizae samples under room temperature (25C) and short-time (15 min) were comparable those obtained at high temperature (100C) and long-time (120 min). Under the same temperature (100C), the extraction yields of berberine from the micronized Phellodendron samples in 15-30 min were comparable to those obtained in 120 min. Overall, the results confirmed that appropriate micronization treatment was able to increase the yields of water-soluble extracts and bioactive compounds from the micronized Glycyrrhizae and Phellodendron samples in a shorter time and probably with lower energy consumption. These findings would be useful to exploit as well as to understand the potential applications of micronization treatment in the botanical industry as well as the herbal extraction process.
URI: http://hdl.handle.net/11455/52016
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