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標題: 戴奧辛誘發人類乳癌細胞株(MCF-7及MDA-MB-231 cells)細胞毒性及核酸氧化損壞作用之研究
Induction of cytotoxicity, oxidative stress and DNA damage in human MCF-7 and MDA-MB-231 breast cancer cells by 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD)
作者: 黃群真
Huang, Chuan-Chen
關鍵字: TCDD
breast cancer
oxidative stress
DNA damage
出版社: 環境工程學系所
引用: 一、 中文部份 陳健民。環境毒物學(Environmental toxicology)。新文京開發出版有限公司。民國91年2月。 林天送、楊明珠。環境荷爾蒙危機。元氣齋出版社有限公司。2001年(民國90年)3月。 陳永仁。環境荷爾蒙管制。財團法人孫運睿學術基金會。民國90年11月。 方如萍,「雌性激素(17b-estradiol)於人類乳癌細胞株誘發基因表現失衡及核酸氧化損害作用之研究」(國立中興大學環境工程研究所,碩士論文,民93年)。 二、西文部份 Abbott,B.D., Buckalew,A.R., DeVito,M.J., Ross,D., Bryant,P.L. and Schmid,J.E. (2003) EGF and TGF-alpha expression influence the developmental toxicity of TCDD: dose response and AhR phenotype in EGF, TGF-alpha, and EGF + TGF-alpha knockout mice. Toxicol.Sci., 71, 84-95. Alsharif, N. Z., Lawson, T., and Stohs, S. J. (1994). Oxidative stress induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin is mediated by the aryl hydrocarbon (Ah) receptor complex. Toxicology 92, 39-51. Ambrosone,C.B., Freudenheim,J.L., Thompson,P.A., Bowman,E., Vena,J.E., Marshall,J.R., Graham,S., Laughlin,R., Nemoto,T. and Shields,P.G. (1999) Manganese superoxide dismutase (MnSOD) genetic polymorphisms, dietary antioxidants, and risk of breast cancer. Cancer Res., 59, 602-606. Ambrosone,C.B., Freudenheim,J.L., Graham,S., Marshall,J.R., Vena,J.E., Brasure,J.R., Laughlin,R., Nemoto,T., Michalek,A.M. and Harrington,A. (1995) Cytochrome P4501A1 and glutathione S-transferase (M1) genetic polymorphisms and postmenopausal breast cancer risk. Cancer Res., 55, 3483-3485. Ame, J. C., Rolli, V., Schreiber, V., Niedergang, C., Apiou, F., Decker, P., Muller, S., Hoger, T., Menissier-de Murcia, J., and de Murcia, G. (1999). PARP-2, A novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase. J Biol Chem 274, 17860-8. Andersen, M. E., Birnbaum, L. S., Barton, H. A., and Eklund, C. R. (1997). Regional hepatic CYP1A1 and CYP1A2 induction with 2,3,7,8-tetrachlorodibenzo-p-dioxin evaluated with a multicompartment geometric model of hepatic zonation. Toxicol Appl Pharmacol 144, 145-55. Andreoli, S. P. (1989). Mechanisms of endothelial cell ATP depletion after oxidant injury. Pediatr Res 25, 97-101. Angus, W. G., Larsen, M. C., and Jefcoate, C. R. (1999). Expression of CYP1A1 and CYP1B1 depends on cell-specific factors in human breast cancer cell lines: role of estrogen receptor status. Carcinogenesis 20, 947-55. Arellano, L. O., Wang, X., and Safe, S. (1993). Effects of cycloheximide on the induction of CYP1A1 gene expression by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in three human breast cancer cell lines. Carcinogenesis 14, 219-22. Arts, J., Kuiper, G.G., Janssen, J.M., Gustafsson, J.A., Lowik, C.W., Pols, H.A. and van Leeuwen, J.P. (1997) Differential expression of estrogen receptors alpha and beta mRNA during differentiation of human osteoblast SV-HFO cells. Endocrinology, 138, 5067-5070. Babiychuk, E., Cottrill, P. B., Storozhenko, S., Fuangthong, M., Chen, Y., O''Farrell, M. K., Van Montagu, M., Inze, D., and Kushnir, S. (1998). Higher plants possess two structurally different poly(ADP-ribose) polymerases. Plant J 15, 635-45. Baccarelli, A., Hirt, C., Pesatori, A. C., Consonni, D., Patterson, D. G.,Jr, Bertazzi, P. A., Dolken, G., and Landi, M. T. (2006). t(14;18) translocations in lymphocytes of healthy dioxin-exposed individuals from Seveso, Italy. Carcinogenesis Baccarelli, A., Pesatori, A. C., Consonni, D., Mocarelli, P., Patterson, D. G.,Jr, Caporaso, N. E., Bertazzi, P. A., and Landi, M. T. (2005). Health status and plasma dioxin levels in chloracne cases 20 years after the Seveso, Italy accident. Br J Dermatol 152, 459-65. Bagchi, D., Shara, M. A., Bagchi, M., Hassoun, E. A., and Stohs, S. J. (1993). Time-dependent effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on serum and urine levels of malondialdehyde, formaldehyde, acetaldehyde, and acetone in rats. Toxicol Appl Pharmacol 123, 83-8. Bauman, J. W., Goldsworthy, T. L., Dunn, C. S., and Fox, T. R. (1995). Inhibitory effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on rat hepatocyte proliferation induced by 2/3 partial hepatectomy. Cell Prolif 28, 437-51. Becher, H., Flesch-Janys, D., Kauppinen, T., Kogevinas, M., Steindorf, K., Manz, A., and Wahrendorf, J. (1996). Cancer mortality in German male workers exposed to phenoxy herbicides and dioxins. Cancer Causes Control 7, 312-21. Beckman, K. B., and Ames, B. N. (1998). Mitochondrial aging: open questions. Ann N Y Acad Sci 854, 118-27. Beckman, K. B., and Ames, B. N. (1997). Oxidative decay of DNA. J Biol Chem 272, 19633-6. Berger, N. A. (1985). Poly(ADP-ribose) in the cellular response to DNA damage. Radiat Res 101, 4-15. Berger, S. J., Sudar, D. C., and Berger, N. A. (1986). Metabolic consequences of DNA damage: DNA damage induces alterations in glucose metabolism by activation of poly (ADP-ribose) polymerase. Biochem Biophys Res Commun 134, 227-32. Berghammer, H., Ebner, M., Marksteiner, R., and Auer, B. (1999). pADPRT-2: a novel mammalian polymerizing(ADP-ribosyl)transferase gene related to truncated pADPRT homologues in plants and Caenorhabditis elegans. FEBS Lett 449, 259-63. Bertazzi, P. A., Bernucci, I., Brambilla, G., Consonni, D., and Pesatori, A. C. (1998). The Seveso studies on early and long-term effects of dioxin exposure: a review. Environ Health Perspect 106 Suppl 2, 625-33. Bertazzi, P. A., Consonni, D., Bachetti, S., Rubagotti, M., Baccarelli, A., Zocchetti, C., and Pesatori, A. C. (2001). Health effects of dioxin exposure: a 20-year mortality study. Am J Epidemiol 153, 1031-44. Bertazzi, P. A., Zocchetti, C., Guercilena, S., Consonni, D., Tironi, A., Landi, M. T., and Pesatori, A. C. (1997). Dioxin exposure and cancer risk: a 15-year mortality study after the "Seveso accident". Epidemiology 8, 646-52. Bock, K. W. (1994). Aryl hydrocarbon or dioxin receptor: biologic and toxic responses. Rev Physiol Biochem Pharmacol 125, 1-42. Bomhard, E. M., Bremmer, J. N., and Herbold, B. A. (1992). Review of the mutagenicity/genotoxicity of butylated hydroxytoluene. Mutat Res 277, 187-200. Breen, A. P., and Murphy, J. A. (1995). Reactions of oxyl radicals with DNA. Free Radic Biol Med 18, 1033-77. Broccardo, C. J., Billings, R. E., Chubb, L. S., Andersen, M. E., and Hanneman, W. H. (2004). Single cell analysis of switch-like induction of CYP1A1 in liver cell lines. Toxicol Sci 78, 287-94. Buchmann, A., Stinchcombe, S., Korner, W., Hagenmaier, H., and Bock, K. W. (1994). Effects of 2,3,7,8-tetrachloro- and 1,2,3,4,6,7,8-heptachlorodibenzo-p-dioxin on the proliferation of preneoplastic liver cells in the rat. Carcinogenesis 15, 1143-50. Bursch, W., Grasl-Kraupp, B., Ellinger, A., Torok, L., Kienzl, H., Mullauer, L., and Schulte-Hermann, R. (1994). Active cell death: role in hepatocarcinogenesis and subtypes. Biochem Cell Biol 72, 669-75. Cadenas, E., and Davies, K. J. (2000). Mitochondrial free radical generation, oxidative stress, and aging. Free Radic Biol Med 29, 222-30. Canter, D. A., M. Lorber, C. T. Braverman, R. O. Warwick, J. F. Walsh, and S. T. Washburn, (1998) “Determining the “MARGIN OF INCREMENTAL EXPOSURE”: An Approach to Assessing Non-cancer Health Effects of Dioxins,” Organohalogen Compounds, 38, 313-317. Cantor, S. B., Bell, D. W., Ganesan, S., Kass, E. M., Drapkin, R., Grossman, S., Wahrer, D. C., Sgroi, D. C., Lane, W. S., Haber, D. A., and Livingston, D. M. (2001). BACH1, a novel helicase-like protein, interacts directly with BRCA1 and contributes to its DNA repair function. Cell 105, 149-60. Carson, D. A., Seto, S., Wasson, D. B., and Carrera, C. J. (1986). DNA strand breaks, NAD metabolism, and programmed cell death. Exp Cell Res 164, 273-81. Caulfield, J. L., Wishnok, J. S., and Tannenbaum, S. R. (1998). Nitric oxide-induced deamination of cytosine and guanine in deoxynucleosides and oligonucleotides. J Biol Chem 273, 12689-95. Cerda, S. R., Turk, P. W., Thor, A. D., and Weitzman, S. A. (1998). Altered expression of the DNA repair protein, N-methylpurine-DNA glycosylase (MPG) in breast cancer. FEBS Lett 431, 12-8. Cerutti, P. A. (1985). Prooxidant states and tumor promotion. Science 227, 375-81. CHAMBON, P., WEILL, J. D., and MANDEL, P. (1963). Nicotinamide mononucleotide activation of new DNA-dependent polyadenylic acid synthesizing nuclear enzyme. Biochem Biophys Res Commun 11, 39-43. Chan, C. Y., Kim, P. M., and Winn, L. M. (2004). TCDD-induced homologous recombination: the role of the Ah receptor versus oxidative DNA damage. Mutat Res 563, 71-9. Chen, H. L., Lee, C. C., Liao, P. C., Guo, Y. L., Chen, C. H., and Su, H. J. (2003). Associations between dietary intake and serum polychlorinated dibenzo-p-dioxin and dibenzofuran (PCDD/F) levels in Taiwanese. Environ Res 91, 172-8. Chen,Y., Liu,X., Pisha,E. et al. (2000) A metabolite of equine estrogens, 4-hydroxyequilenin, induces DNA damage and apoptosis in breast cancer cell lines. Chem. Res. Toxicol., 13, 342–350. Christensen, J. G., Gonzales, A. J., Cattley, R. C., and Goldsworthy, T. L. (1998). Regulation of apoptosis in mouse hepatocytes and alteration of apoptosis by nongenotoxic carcinogens. Cell Growth Differ 9, 815-25. Cleaver, J. E. (1968). Defective repair replication of DNA in xeroderma pigmentosum. Nature 218, 652-6. Couse, J.F. and Korach, K.S. (1999) Estrogen receptor null mice: what have we learned and where will they lead us? Endocr.Rev., 20, 358-417. Crump, K. S., Canady, R., and Kogevinas, M. (2003). Meta-analysis of dioxin cancer dose response for three occupational cohorts. Environ Health Perspect 111, 681-7. Dalton, T. P., Chen, Y., Schneider, S. N., Nebert, D. W., and Shertzer, H. G. (2004). Genetically altered mice to evaluate glutathione homeostasis in health and disease. Free Radic Biol Med 37, 1511-26. Dalton, T. P., Puga, A., and Shertzer, H. G. (2002). Induction of cellular oxidative stress by aryl hydrocarbon receptor activation. Chem Biol Interact 141, 77-95. Dantzer, F., Nasheuer, H. P., Vonesch, J. L., de Murcia, G., and Menissier-de Murcia, J. (1998). Functional association of poly(ADP-ribose) polymerase with DNA polymerase alpha-primase complex: a link between DNA strand break detection and DNA replication. Nucleic Acids Res 26, 1891-8. De Rosa, C. T., Brown, D., Dhara, R., Garrett, W., Hansen, H., Holler, J., Jones, D., Jordan-Izaguirre, D., O''Connor, R., Pohl, H., and Xintaras, C. (1997). Dioxin and dioxin-like compounds in soil, Part I: ATSDR interim policy guideline. Agency for Toxic Substances and Disease Registry. Toxicol Ind Health 13, 759-68. De Rosa, C. T., Brown, D., Dhara, R., Garrett, W., Hansen, H., Holler, J., Jones, D., Jordan-Izaguirre, D., O''Connor, R., Pohl, H., and Xintaras, C. (1997). Dioxin and dioxin-like compounds in soil, Part II: Technical support document for ATSDR interim policy guideline. Toxicol Ind Health 13, 769-804. Denecker, G., Vercammen, D., Declercq, W., and Vandenabeele, P. (2001). Apoptotic and necrotic cell death induced by death domain receptors. Cell Mol Life Sci 58, 356-70. deRojas-Walker, T., Tamir, S., Ji, H., Wishnok, J. S., and Tannenbaum, S. R. (1995). Nitric oxide induces oxidative damage in addition to deamination in macrophage DNA. Chem Res Toxicol 8, 473-7. DeVito, M. J., Birnbaum, L. S., Farland, W. H., and Gasiewicz, T. A. (1995). Comparisons of estimated human body burdens of dioxinlike chemicals and TCDD body burdens in experimentally exposed animals. Environ Health Perspect 103, 820-31. Diaz, L.K. and Sneige, N. (2005) Estrogen receptor analysis for breast cancer: current issues and keys to increasing testing accuracy. Adv.Anat.Pathol., 12, 10-19. Dizdaroglu, M. (1992). Oxidative damage to DNA in mammalian chromatin. Mutat Res 275, 331-42. Douki, T., and Cadet, J. (1996). Peroxynitrite mediated oxidation of purine bases of nucleosides and isolated DNA. Free Radic Res 24, 369-80. Dreher, D., and Junod, A. F. (1996). Role of oxygen free radicals in cancer development. Eur J Cancer 32A, 30-8. Elledge, S. J. (1996). Cell cycle checkpoints: preventing an identity crisis. Science 274, 1664-72. Enmark, E., Pelto-Huikko, M., Grandien, K., Lagercrantz, S., Lagercrantz, J., Fried, G., Nordenskjold, M. and Gustafsson, J.A. (1997) Human estrogen receptor beta-gene structure, chromosomal localization, and expression pattern. J.Clin.Endocrinol.Metab., 82, 4258-4265. Epe, B., Ballmaier, D., Roussyn, I., Briviba, K., and Sies, H. (1996). DNA damage by peroxynitrite characterized with DNA repair enzymes. Nucleic Acids Res 24, 4105-10. Esterbauer, H., Zollner, H., and Lang, J. (1985). Metabolism of the lipid peroxidation product 4-hydroxynonenal by isolated hepatocytes and by liver cytosolic fractions. Biochem J 228, 363-73. Fernet, M., Ponette, V., Deniaud-Alexandre, E., Menissier-De Murcia, J., De Murcia, G., Giocanti, N., Megnin-Chanet, F., and Favaudon, V. (2000). Poly(ADP-ribose) polymerase, a major determinant of early cell response to ionizing radiation. Int J Radiat Biol 76, 1621-9. Fiers, W., Beyaert, R., Declercq, W., and Vandenabeele, P. (1999). More than one way to die: apoptosis, necrosis and reactive oxygen damage. Oncogene 18, 7719-30. Fingerhut, M. A., Halperin, W. E., Marlow, D. A., Piacitelli, L. A., Honchar, P. A., Sweeney, M. H., Greife, A. L., Dill, P. A., Steenland, K., and Suruda, A. J. (1991). Cancer mortality in workers exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin. N Engl J Med 324, 212-8. Finkel, T., and Holbrook, N. J. (2000). Oxidants, oxidative stress and the biology of ageing. Nature 408, 239-47. Flesch-Janys, D., Steindorf, K., Gurn, P., and Becher, H. (1998). Estimation of the cumulated exposure to polychlorinated dibenzo-p-dioxins/furans and standardized mortality ratio analysis of cancer mortality by dose in an occupationally exposed cohort. Environ Health Perspect 106 Suppl 2, 655-62. Fox, T. R., Best, L. L., Goldsworthy, S. M., Mills, J. J., and Goldsworthy, T. L. (1993). Gene expression and cell proliferation in rat liver after 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure. Cancer Res 53, 2265-71. Gottlicher, M., and Wiebel, F. J. (1991). 2,3,7,8-Tetrachlorodibenzo-p-dioxin causes unbalanced growth in 5L rat hepatoma cells. Toxicol Appl Pharmacol 111, 496-503. Grassman, J. A., Masten, S. A., Walker, N. J., and Lucier, G. W. (1998). Animal models of human response to dioxins. Environ Health Perspect 106 Suppl 2, 761-75. Gutteridge, S., and Robb, D. (1975). The catecholase activity of Neurospora tyrosinase. Eur J Biochem 54, 107-16. Ha, H. C., and Snyder, S. H. (2000). Poly(ADP-ribose) polymerase-1 in the nervous system. Neurobiol Dis 7, 225-39. Harper, N., Howie, L., Connor, K., Arellano, L., Craig, A., Dickerson, R., and Safe, S. (1993). Immunosuppressive and monooxygenase induction activities of highly chlorinated diphenyl ether congeners in C57BL/6 and DBA/2 mice. Fundam Appl Toxicol 20, 496-502. Harrad, S., Wang, Y., Sandaradura, S., and Leeds, A. (2003). Human dietary intake and excretion of dioxin-like compounds. J Environ Monit 5, 224-8. Harrad, S. J., and Smith, D. J. (1997). Evaluation of a terrestrial food chain model for estimating foodstuff concentrations of PCDD/Fs. Chemosphere 34, 1723-37. Hassoun, E. A., Al-Ghafri, M., and Abushaban, A. (2003). The role of antioxidant enzymes in TCDD-induced oxidative stress in various brain regions of rats after subchronic exposure. Free Radic Biol Med 35, 1028-36. Hassoun, E. A., Li, F., Abushaban, A., and Stohs, S. J. (2000). The relative abilities of TCDD and its congeners to induce oxidative stress in the hepatic and brain tissues of rats after subchronic exposure. Toxicology 145, 103-13. Hassoun, E. A., and Stohs, S. J. (1996). TCDD, endrin and lindane induced oxidative stress in fetal and placental tissues of C57BL/6J and DBA/2J mice. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 115, 11-8. Hassoun, E. A., Walter, A. C., Alsharif, N. Z., and Stohs, S. J. (1997). Modulation of TCDD-induced fetotoxicity and oxidative stress in embryonic and placental tissues of C57BL/6J mice by vitamin E succinate and ellagic acid. Toxicology 124, 27-37. Hassoun, E. A., Wang, H., Abushaban, A., and Stohs, S. J. (2002). Induction of oxidative stress in the tissues of rats after chronic exposure to TCDD, 2,3,4,7,8-pentachlorodibenzofuran, and 3,3'',4,4'',5-pentachlorobiphenyl. J Toxicol Environ Health A 65, 825-42. Hassoun, E. A., Wilt, S. C., Devito, M. J., Van Birgelen, A., Alsharif, N. Z., Birnbaum, L. S., and Stohs, S. J. (1998). Induction of oxidative stress in brain tissues of mice after subchronic exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol Sci 42, 23-7. Henle, E. S., and Linn, S. (1997). Formation, prevention, and repair of DNA damage by iron/hydrogen peroxide. J Biol Chem 272, 19095-8. Hensley, K., Kotake, Y., Sang, H., Pye, Q. N., Wallis, G. L., Kolker, L. M., Tabatabaie, T., Stewart, C. A., Konishi, Y., Nakae, D., and Floyd, R. A. (2000). Dietary choline restriction causes complex I dysfunction and increased H(2)O(2) generation in liver mitochondria. Carcinogenesis 21, 983-9. Herceg, Z., and Wang, Z. Q. (1999). Failure of poly(ADP-ribose) polymerase cleavage by caspases leads to induction of necrosis and enhanced apoptosis. Mol Cell Biol 19, 5124-33. Hilscherova, K., Blankenship, A. L., Nie, M., Coady, K. K., Upham, B. L., Trosko, J. E., and Giesy, J. P. (2003). Oxidative stress in liver and brain of the hatchling chicken (Gallus domesticus) following in ovo injection with TCDD. Comp Biochem Physiol C Toxicol Pharmacol 136, 29-45. Hoffman, E. C., Reyes, H., Chu, F. F., Sander, F., Conley, L. H., Brooks, B. A., and Hankinson, O. (1991). Cloning of a factor required for activity of the Ah (dioxin) receptor. Science 252, 954-8. Huang, L. Q., Tong, H., and Donnelly, J. R. (1992). Characterization of dibromopolychlorodibenzo-p-dioxins and dibromopolychlorodibenzofurans in municipal waste incinerator fly ash using gas chromatography/mass spectrometry. Anal Chem 64, 1034-40. Huff, J., Lucier, G., and Tritscher, A. (1994). Carcinogenicity of TCDD: experimental, mechanistic, and epidemiologic evidence. Annu Rev Pharmacol Toxicol 34, 343-72. Hughes, H., Smith, C. V., Horning, E. C., and Mitchell, J. R. (1983). High-performance liquid chromatography and gas chromatography-mass spectrometry determination of specific lipid peroxidation products in vivo. Anal Biochem 130, 431-6. Hushka, D. R., and Greenlee, W. F. (1995). 2,3,7,8-Tetrachlorodibenzo-p-dioxin inhibits DNA synthesis in rat primary hepatocytes. Mutat Res 333, 89-99. Inoue, S., and Kawanishi, S. (1995). Oxidative DNA damage induced by simultaneous generation of nitric oxide and superoxide. FEBS Lett 371, 86-8. Jenson, E.V. and Jacobson, H.I. (1960) In: Pincus G. Vollmer (Eds.), Biological activities of steroids in relation to cancer. Acad. Pre. 161-178. Jiricny, J., and Marra, G. (2003). DNA repair defects in colon cancer. Curr Opin Genet Dev 13, 61-9. Johansson, M. (1999). A human poly(ADP-ribose) polymerase gene family (ADPRTL): cDNA cloning of two novel poly(ADP-ribose) polymerase homologues. Genomics 57, 442-5. Kennedy, R. D., Quinn, J. E., Johnston, P. G., and Harkin, D. P. (2002). BRCA1: mechanisms of inactivation and implications for management of patients. Lancet 360, 1007-14. Khanna, K. K., and Jackson, S. P. (2001). DNA double-strand breaks: signaling, repair and the cancer connection. Nat Genet 27, 247-54. Kim, J. S., He, L., and Lemasters, J. J. (2003). Mitochondrial permeability transition: a common pathway to necrosis and apoptosis. Biochem Biophys Res Commun 304, 463-70. Knerr, S., Schaefer, J., Both, S., Mally, A., Dekant, W., and Schrenk, D. (2006). 2,3,7,8-Tetrachlorodibenzo-p-dioxin induced cytochrome P450s alter the formation of reactive oxygen species in liver cells. Mol Nutr Food Res 50, 378-84. Kociba, R. (1984) Evaluation of the carcinogenic and mutant potential of 2,3,7,8-TCDD and other chlorinated dioxins. Bio. Mech. Dioxin Action 18, 73-84 Kociba, R. J., Keyes, D. G., Beyer, J. E., Carreon, R. M., Wade, C. E., Dittenber, D. A., Kalnins, R. P., Frauson, L. E., Park, C. N., Barnard, S. D., Hummel, R. A., and Humiston, C. G. (1978). Results of a two-year chronic toxicity and oncogenicity study of 2,3,7,8-tetrachlorodibenzo-p-dioxin in rats. Toxicol Appl Pharmacol 46, 279-303. Kogevinas, M. (2001). Human health effects of dioxins: cancer, reproductive and endocrine system effects. Hum Reprod Update 7, 331-9. Kogevinas, M. (2000). Studies of cancer in humans. Food Addit Contam 17, 317-24. Kogevinas, M., Becher, H., Benn, T., Bertazzi, P. A., Boffetta, P., Bueno-de-Mesquita, H. B., Coggon, D., Colin, D., Flesch-Janys, D., Fingerhut, M., Green, L., Kauppinen, T., Littorin, M., Lynge, E., Mathews, J. D., Neuberger, M., Pearce, N., and Saracci, R. (1997). Cancer mortality in workers exposed to phenoxy herbicides, chlorophenols, and dioxins. An expanded and updated international cohort study. Am J Epidemiol 145, 1061-75. Kohle, C., Gschaidmeier, H., Lauth, D., Topell, S., Zitzer, H., and Bock, K. W. (1999). 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)-mediated membrane translocation of c-Src protein kinase in liver WB-F344 cells. Arch Toxicol 73, 152-8. Kress, S., Reichert, J., and Schwarz, M. (1998). Functional analysis of the human cytochrome P4501A1 (CYP1A1) gene enhancer. Eur J Biochem 258, 803-12. Krokan, H. E., Drablos, F., and Slupphaug, G. (2002). Uracil in DNA--occurrence, consequences and repair. Oncogene 21, 8935-48. Krokan, H. E., Nilsen, H., Skorpen, F., Otterlei, M., and Slupphaug, G. (2000). Base excision repair of DNA in mammalian cells. FEBS Lett 476, 73-7. Krokan, H. E., Standal, R., and Slupphaug, G. (1997). DNA glycosylases in the base excision repair of DNA. Biochem J 325 ( Pt 1), 1-16. Kubota, Y., Nash, R. A., Klungland, A., Schar, P., Barnes, D. E., and Lindahl, T. (1996). Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein. EMBO J 15, 6662-70. Kuiper, G.G., Carlsson, B., Grandien, K., Enmark, E., Haggblad, J., Nilsson, S. and Gustafsson, J.A. (1997) Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta. Endocrinology, 138, 863-870. Kuiper, G.G., Lemmen, J.G., Carlsson, B., Corton, J.C., Safe, S.H., van der Saag, P.T., van der Burg, B. and Gustafsson, J.A. (1998a) Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta. Endocrinology, 139, 4252-4263. Kuiper, G.G., Shughrue, P.J., Merchenthaler, I. and Gustafsson, J.A. (1998c) The estrogen receptor beta subtype: a novel mediator of estrogen action in neuroendocrine systems. Front.Neuroendocrinol., 19, 253-286. Kumagami, H. (1994) Sex hormones in the kidney and endolymphatic sac. An immunohistological study. Acta Otolaryngol., 114, 48-51. Lai, K. P., Li, W., Xu, Y., Wong, M. H., and Wong, C. K. (2004). Dioxin-like components in human breast milk collected from Hong Kong and Guangzhou. Environ Res 96, 88-94. Landers, J. P., and Bunce, N. J. (1991). The Ah receptor and the mechanism of dioxin toxicity. Biochem J 276 ( Pt 2), 273-87. Latchoumycandane, C., Chitra, K. C., and Mathur, P. P. (2003). 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD) induces oxidative stress in the epididymis and epididymal sperm of adult rats. Arch Toxicol 77, 280-4. Lautier, D., Lagueux, J., Thibodeau, J., Menard, L., and Poirier, G. G. (1993). Molecular and biochemical features of poly (ADP-ribose) metabolism. Mol Cell Biochem 122, 171-93. Laybourn, P. J., and Kadonaga, J. T. (1992). Threshold phenomena and long-distance activation of transcription by RNA polymerase II. Science 257, 1682-5. Leist, M., Single, B., Naumann, H., Fava, E., Simon, B., Kuhnle, S., and Nicotera, P. (1999). Inhibition of mitochondrial ATP generation by nitric oxide switches apoptosis to necrosis. Exp Cell Res 249, 396-403. Lindahl, T. (1993). Instability and decay of the primary structure of DNA. Nature 362, 709-15. Lindahl, T., and Wood, R. D. (1999). Quality control by DNA repair. Science 286, 1897-905. Lindner, V., Kim, S.K., Karas, R.H., Kuiper, G.G., Gustafsson, J.A. and Mendelsohn, M.E. (1998) Increased expression of estrogen receptor-beta mRNA in male blood vessels after vascular injury. Circ.Res., 83, 224-229. Ling, Y. C., Soong, D. K., and Lee, M. K. (1995). PCDD/DFs and coplanar PCBs in sediment and fish samples from the Er-Jen river in Taiwan. Chemosphere 31, 2863-72. Lowell, B. B., and Spiegelman, B. M. (2000). Towards a molecular understanding of adaptive thermogenesis. Nature 404, 652-60. Lucier, G. W., Tritscher, A., Goldsworthy, T., Foley, J., Clark, G., Goldstein, J., and Maronpot, R. (1991). Ovarian hormones enhance 2,3,7,8-tetrachlorodibenzo-p-dioxin-mediated increases in cell proliferation and preneoplastic foci in a two-stage model for rat hepatocarcinogenesis. Cancer Res 51, 1391-7. Ma, Q., and Whitlock, J. P.,Jr (1996). The aromatic hydrocarbon receptor modulates the Hepa 1c1c7 cell cycle and differentiated state independently of dioxin. Mol Cell Biol 16, 2144-50. Mahajan, P. B., and Zuo, Z. (1998). Purification and cDNA cloning of maize Poly(ADP)-ribose polymerase. Plant Physiol 118, 895-905. Maronpot, R. R., Foley, J. F., Takahashi, K., Goldsworthy, T., Clark, G., Tritscher, A., Portier, C., and Lucier, G. (1993). Dose response for TCDD promotion of hepatocarcinogenesis in rats initiated with DEN: histologic, biochemical, and cell proliferation endpoints. Environ Health Perspect 101, 634-42. Masson, M., Niedergang, C., Schreiber, V., Muller, S., Menissier-de Murcia, J., and de Murcia, G. (1998). XRCC1 is specifically associated with poly(ADP-ribose) polymerase and negatively regulates its activity following DNA damage. Mol Cell Biol 18, 3563-71. Meister, A. (1994). Glutathione, ascorbate, and cellular protection. Cancer Res 54, 1969s-75s. Meister, A. (1994). Glutathione-ascorbic acid antioxidant system in animals. J Biol Chem 269, 9397-400. Melino, G., Catani, M. V., Corazzari, M., Guerrieri, P., and Bernassola, F. (2000). Nitric oxide can inhibit apoptosis or switch it into necrosis. Cell Mol Life Sci 57, 612-22. Mimura, J., and Fujii-Kuriyama, Y. (2003). Xenobiotics and transcriptional regulation. Tanpakushitsu Kakusan Koso 48, 2261-6. Mitra, S., Boldogh, I., Izumi, T., and Hazra, T. K. (2001). Complexities of the DNA base excision repair pathway for repair of oxidative DNA damage. Environ Mol Mutagen 38, 180-90. Mitra, S., Hazra, T. K., Roy, R., Ikeda, S., Biswas, T., Lock, J., Boldogh, I., and Izumi, T. (1997). Complexities of DNA base excision repair in mammalian cells. Mol Cells 7, 305-12. Mitrunen, K., and Hirvonen, A. (2003). Molecular epidemiology of sporadic breast cancer. The role of polymorphic genes involved in oestrogen biosynthesis and metabolism. Mutat Res 544, 9-41. Mobley, J. A., and Brueggemeier, R. W. (2004). Estrogen receptor-mediated regulation of oxidative stress and DNA damage in breast cancer. Carcinogenesis 25, 3-9. Moolgavkar, S. H. (1993). Cell proliferation and carcinogenesis models: general principles with illustrations from the rodent liver system. Environ Health Perspect 101 Suppl 5, 91-4. Moraes, E. C., Keyse, S. M., and Tyrrell, R. M. (1990). Mutagenesis by hydrogen peroxide treatment of mammalian cells: a molecular analysis. Carcinogenesis 11, 283-93. Munzel, P., Bock-Hennig, B., Schieback, S., Gschaidmeier, H., Beck-Gschaidmeier, S., and Bock, K. W. (1996). Growth modulation of hepatocytes and rat liver epithelial cells (WB-F344) by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Carcinogenesis 17, 197-202. Musarrat,J., Arezina-Wilson,J. and Wani,A.A. (1996) Prognostic and aetiological relevance of 8-hydroxyguanosine in human breast carcinogenesis. Eur. J. Cancer, 32A, 1209–1214. Nakamura, J., Walker, V. E., Upton, P. B., Chiang, S. Y., Kow, Y. W., and Swenberg, J. A. (1998). Highly sensitive apurinic/apyrimidinic site assay can detect spontaneous and chemically induced depurination under physiological conditions. Cancer Res 58, 222-5. Neto, J. B., Gentil, A., Cabral, R. E., and Sarasin, A. (1992). Mutation spectrum of heat-induced abasic sites on a single-stranded shuttle vector replicated in mammalian cells. J Biol Chem 267, 19718-23. Nguyen, T., Brunson, D., Crespi, C. L., Penman, B. W., Wishnok, J. S., and Tannenbaum, S. R. (1992). DNA damage and mutation in human cells exposed to nitric oxide in vitro. Proc Natl Acad Sci U S A 89, 3030-4. Nishimura, N., Miyabara, Y., Suzuki, J. S., Sato, M., Aoki, Y., Satoh, M., Yonemoto, J., and Tohyama, C. (2001). Induction of metallothionein in the livers of female Sprague-Dawley rats treated with 2,3,7 ,8-tetrachlorodibenzo-p-dioxin. Life Sci 69, 1291-303. Okey, A. B., Riddick, D. S., and Harper, P. A. (1994). Molecular biology of the aromatic hydrocarbon (dioxin) receptor. Trends Pharmacol Sci 15, 226-32. Oleinick, N. L., and Evans, H. H. (1985). Poly(ADP-ribose) and the response of cells to ionizing radiation. Radiat Res 101, 29-46. Österlund, M., Kuiper, G.G., Gustafsson, J.A. and Hurd, Y.L. (1998) Differential distribution and regulation of estrogen receptor-alpha and -beta mRNA within the female rat brain. Brain Res.Mol.Brain Res., 54, 175-180. Ott, M. G., and Zober, A. (1996). Cause specific mortality and cancer incidence among employees exposed to 2,3,7,8-TCDD after a 1953 reactor accident. Occup Environ Med 53, 606-12. Parke, D. V., Ioannides, C., and Lewis, D. F. (1991). The 1990 Pharmaceutical Manufacturers Association of Canada keynote lecture. The role of the cytochromes P450 in the detoxication and activation of drugs and other chemicals. Can J Physiol Pharmacol 69, 537-49. Pesatori, A. C., Consonni, D., Bachetti, S., Zocchetti, C., Bonzini, M., Baccarelli, A., and Bertazzi, P. A. (2003). Short- and long-term morbidity and mortality in the population exposed to dioxin after the "Seveso accident". Ind Health 41, 127-38. Pesatori, A. C., Consonni, D., Tironi, A., Zocchetti, C., Fini, A., and Bertazzi, P. A. (1993). Cancer in a young population in a dioxin-contaminated area. Int J Epidemiol 22, 1010-3. Pesatori, A. C., Zocchetti, C., Guercilena, S., Consonni, D., Turrini, D., and Bertazzi, P. A. (1998). Dioxin exposure and non-malignant health effects: a mortality study. Occup Environ Med 55, 126-31. Pieper, A. A., Brat, D. J., Krug, D. K., Watkins, C. C., Gupta, A., Blackshaw, S., Verma, A., Wang, Z. Q., and Snyder, S. H. (1999). Poly(ADP-ribose) polymerase-deficient mice are protected from streptozotocin-induced diabetes. Proc Natl Acad Sci U S A 96, 3059-64. Pieper, A. A., Verma, A., Zhang, J., and Snyder, S. H. (1999). Poly (ADP-ribose) polymerase, nitric oxide and cell death. Trends Pharmacol Sci 20, 171-81. Pierce, A. J., Stark, J. M., Araujo, F. D., Moynahan, M. E., Berwick, M., and Jasin, M. (2001). Double-strand breaks and tumorigenesis. Trends Cell Biol 11, S52-9. Pitot, H. C. (1995). The role of receptors in multistage carcinogenesis. Mutat Res 333, 3-14. Pitot, H. C. (1995). The tamoxifen controversy--clinical chemopreventive agent and experimental carcinogen. Proc Soc Exp Biol Med 208, 139-40. Pitot, H. C., and Dragan, Y. P. (1993). Stage of tumor progression, progressor agents, and human risk. Proc Soc Exp Biol Med 202, 37-43. Pleschke, J. M., Kleczkowska, H. E., Strohm, M., and Althaus, F. R. (2000). Poly(ADP-ribose) binds to specific domains in DNA damage checkpoint proteins. J Biol Chem 275, 40974-80. Poellinger, L. (2000). Mechanistic aspects--the dioxin (aryl hydrocarbon) receptor. Food Addit Contam 17, 261-6. Poland, A., and Glover, E. (1979). An estimate of the maximum in vivo covalent binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin to rat liver protein, ribosomal RNA, and DNA. Cancer Res 39, 3341-4. Poland, A., and Knutson, J. C. (1982). 2,3,7,8-tetrachlorodibenzo-p-dioxin and related halogenated aromatic hydrocarbons: examination of the mechanism of toxicity. Annu Rev Pharmacol Toxicol 22, 517-54. Prabhakaran, K., Li, L., Borowitz, J. L., and Isom, G. E. (2004). Caspase inhibition switches the mode of cell death induced by cyanide by enhancing reactive oxygen species generation and PARP-1 activation. Toxicol Appl Pharmacol 195, 194-202. Proskuryakov, S. Y., Konoplyannikov, A. G., and Gabai, V. L. (2003). Necrosis: a specific form of programmed cell death? Exp Cell Res 283, 1-16. Reichard, J. F., Dalton, T. P., Shertzer, H. G., and Puga, A. (2005) in Nonlinearity in Biology and Toxicology and Medicine (Calabrese, E. J., ed) CRC Press, Boca Raton, FL, in press. Reiners, J. J.,Jr, and Clift, R. E. (1999). Aryl hydrocarbon receptor regulation of ceramide-induced apoptosis in murine hepatoma 1c1c7 cells. A function independent of aryl hydrocarbon receptor nuclear translocator. J Biol Chem 274, 2502-10. Revich, B. A., Sotskov, I., Kliuev, N. A., Brodskii, E. S., Lipchenko, I., Muzurov, I. V., and Zeilert, V. I. (2001). Dioxins in the environment and in the blo
摘要: 本研究之主旨在於探討環境中之持久性有機污染物(persistent organic pollutants, POPs)中毒性最大之戴奧辛2,3,7,8-四氯戴奧辛(2,3,7,8-tetrachlorodibenzo-p-dioxin, TCDD),於人類乳癌細胞株ERα(+)/MCF-7 與 ERα(-)/MDA-MB-231細胞,誘發細胞毒性、氧化壓力及核酸氧化損害作用之研究。研究結果顯示,MCF-7 與MDA-MB-231細胞所誘發之細胞毒性皆具有時間及劑量之效應。TCDD於短時間內即可使MCF-7與MDA-MB-231細胞累積明顯之ROS,但其所誘發之ROS並未隨反應時間之增加而有持續累積之現象。實驗中添加NEM(glutathione移除劑)可增加TCDD於細胞內ROS之累積量。TCDD可於短時間內降低此兩株細胞中GSH含量,但延長反應時間後GSH含量則恢復正常,此顯示細胞中ROS累積量之變化可能與GSH含量之增減有關。將MCF-7細胞於ROS試驗中添加4-OHT(ERα之antagonist),其所得之ROS累積量與MDA-MB-231細胞約略相同,其顯示ERα確實會影響由TCDD誘發之相關效應。MDA-MB-231細胞中添加α-NF(AhR之antagonist)可抑制由TCDD所誘發之ROS生成,因而推論TCDD是經由與AhR反應,進而增加細胞內ROS之累積。上述之實驗結果顯示TCDD於MCF-7細胞中誘發細胞毒性之能力較強,而其於MDA-MB-231細胞中誘發ROS累積及氧化核酸損害之程度皆較MCF-7細胞敏感。在TCDD誘發細胞中DNA損害方面,皆可使MCF-7與MDA-MB-231細胞誘發ADL之生成(p≦0.01),但此種類之核酸損害並不會持續的累積於細胞中,且於細胞所誘發之ADL大部分可被putrescine所切除。本實驗亦證實TCDD會造成MCF-7與MDA-MB-231細胞中NAD(P)H及NAD+之下降(約70 ~ 80%),而PRPA-1 〔poly(ADP-ribose)polymerase-1〕抑制劑之存在,可以抑制此一現象。此一結果顯示,TCDD之暴露可誘發MCF-7與MDA-MB-231細胞之核酸股斷鏈(DNA strand breaks)。綜合上述結果,TCDD可於人類乳癌細胞株誘發不同程度之細胞毒性、ROS形成及氧化核酸損害,而ERα可能於其中扮演重要之角色。
The objective of this research is to examine the induction of oxidative stress, DNA damage, and cell toxicity by one of the most toxic persistent organic pollutants (POPs), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), in human ERα(+)/MCF-7 and ERα(-)/MDA-MB-231 breast cancer cells. Results indicated that TCDD induced concentration- and time-dependent increases in cytotoxic response in MCF-7 and MDA-MB-231 cells. Further, we detected that a 4–fold increase in ROS formation was detected in MDA-MB-231 cells exposed to TCDD (10 nM) when compared to control (p≦0.05). Similar observation was also detected in MCF-7 cells. Additionally, we confirmed that TCDD induced a decrease in intracellular GSH in both MDA-MB-231 and MCF-7 cells. The data also indicated that TCDD induced an increase in the number of aldehydic DNA lesions (ADLs) in MDA-MB-231 and MCF-7 cells. Further characterization confirmed that the ADL induced by TCDD in MCF-7 and MDA-MB-231 cells was predominantly putrescine-excisable ADL. Overall, the extent of cytotoxic response was greater in MCF-7 cells than in MDA-MB-231 cells whereas the extent of ROS formation, GSH depletion, and ADL formation were greater in MDA-MB-231 cells than in MCF-7 cells. In addition, when cells were exposed to TCDD at non-cytotoxic concentration, we observed that TCDD (1 nM) alone induce decreases in intracellular NAD(P)H and NAD+ in MDA-MB-231 and MCF-7 cells. Further investigation indicated that the TCDD-induced depletion of NAD(P)H in MDA-MB-231 and MCF-7 cells was completely blocked by three types of PARP-1〔poly(ADP-ribose)polymerase-1〕inhibitors. This evidence suggests that TCDD induced decreases in intracellular NAD(P)H and NAD+ through PARP-1 activation mediated by formation of DNA strand breaks. In conclusions, this evidence suggests that TCDD may induce ROS formation, oxidative DNA lesions, and cell death in human breast cancer cells. The data also suggests that the status of estrogen receptor α may play a role in modulating the TCDD-induced oxidative DNA damage and cell death in human breast cancer cells.
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