Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/52111
標題: Adherence of Lactobacillus acidophilus LAP5 to host intestine cells and the antagonistic effect of LAP5 on the chicken infection by different Salmonella serotypes.
嗜乳酸桿菌LAP5對宿主細胞之吸附特性及對不同血清型沙門氏菌感染雞隻之抑制作用
作者: Ho, Ya-Wen
何雅文
關鍵字: chicken

Probiotics
Salmonella.
益生菌
沙門氏菌
出版社: 食品暨應用生物科技學系所
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摘要: The objective of this study was to evaluate the possibility to use a Lactobacillus acidophilus strain as feed supplement and antibiotic use. This strains, ie, LAP5, was able to inhibit 17 Salmonella strains tested. Also, it demonstrated high adherence to human intestine epithelium cells Caco-2 as well as chicken crop epithelium cells. When three days aged chicken were fed with strain LAP5 200μl (109 cfu/mL ) continuously for 7 days and afterwards, challenged with Salmonella 200μl (109 cfu/mL ) for six days, results showed that Salmonella cells invaded in chicken liver and spleen were about 2 and 5 log CFU/g organ respectively. For LAP5 fed chickens, Salmonella cells invaded in liver and spleen reduced 1~2 log CFU/g organ (P<0.05) . Since LAP5 strain was able to inhibit the growth of pathogenic bacteria, with adherence and able to reduce the Salmonella infection through competitive expelling effect, the strain LAP5 could be used for probiotic use.
本研究目的在評估乳酸桿菌 Lactobacillus acidophilus LAP5 作為益生菌與取代抗生素用於雞隻飼養之可行性。於體外試驗中, LAP5 對本實驗 17 株沙門氏菌皆具抑菌效果。吸附試驗中以 LAP5 對雞隻嗉囊上皮細胞、腸道上皮及人類腸道上皮細胞株 Caco-2 具強吸附力,故以 LAP5 作為本研究之試驗菌株。於動物試驗中,鷄隻於三日齡時口投 LAP5 菌株 200 μl ( 109 cfu/mL )連續餵食七天,於 9日齡口投感染沙門氏菌菌液 200 μl (109 cfu/mL ) 連續餵食六天。結果顯示,感染後的雞隻肝臟與脾臟之菌數約達到 2-5 log cfu/g,餵食 LAP5 7 天後,雞隻肝臟與脾臟之菌數可下降 1-2 log cfu/g,且脾臟的保護效果高於肝臟 (P<0.05)。綜上所述, LAP5 菌株具有抑菌、吸附雞隻嗉囊上皮、雞隻腸道上皮細胞及人類腸道上皮細胞株 Caco-2 細胞之能力,且對沙門氏菌亦有競爭排除之效果,因此預期可作為益生菌之候選菌株。
URI: http://hdl.handle.net/11455/52111
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