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標題: Adherence of Lactobacillus acidophilus LAP5 to host intestine cells and the antagonistic effect of LAP5 on the chicken infection by different Salmonella serotypes.
作者: Ho, Ya-Wen
關鍵字: chicken

出版社: 食品暨應用生物科技學系所
引用: 王怡仁、杜明勳。2003。益生菌。基層醫學18(3):72-75。 王芷宜。2007。篩選具抑制沙門氏菌之乳酸菌及其免疫效果評估。碩士論文。國立中興大學。 邱美雲。2003。鷄隻腸道來源之乳酸桿菌 Lactobacillus reuteri Pg4菌株作為益生菌之探討。碩士論文。國立中興大學。 邱雪惠。2004。乳酸菌之抗癌機制。食品工業。36(3):27-33. 吳兩新。2008。中草藥作為雞隻飼料添加劑之可行性研究。中醫藥年報。26(6):269-304. 林文鑫。1999。動物來源乳酸菌之特性及免疫保健機能之初步研究。碩士論文。國立中興大學。 連耿汶。2009。乳酸菌抑制沙門氏菌屬感染及侵入雞隻臟器之探討。 碩士論文。弘光科技大學。 陳慶源,林富美。2004。益生菌之保健功效。食品工業。36 (3): 1-3。 陳慶源、黃崇真、邱雪惠、廖啟成。2007。乳酸菌之保健功效與產品開發。農業生技產業季刊。11:60-68. 蔡政志。2004。乳酸菌之分子鑑定及其特性與機能性評估。博士論文。國立中興大學。 蕭富升。2004。轉殖殖β-聚葡萄糖酶基因之Lactobacillus reuteri Pg4作為家禽益生菌之生物有效性評估。碩士論文。國立中興大學。 Annika, M. M., M. Manninen, and H. Gylienberg. 1983. The adherence of lactic acid bacteria to the columnar epithelial cells of pigs and calves. J. Appl. Bacteriol. 55: 241-245. Arunachalam S. 1999. Informatics in clinical practice in developing countries: stillearly days. BMJ. 13;319(7220):1297. Ayyagari A, Agarwal J, Garg A. 2003. Antibiotic associated diarrhoea: Infectious causes. Indian J Med Microbiol. 21(1):6-11. Barnes, H. J. 1987. Escherichia coli problems in poultry production. In Biotechnology in Feed industry ed. Lyons, T. P. pp.333-338. Kentucky:Alltech Technical Publication. Barrow, P. A., M. B. Huggins, and M. A. Lovell. 1994. Host specificity of Salmonella infection in chickens and mice is expressed in vivo primarily at the level of the reticuloendothelial system. Ameri. Soci. Microbiol. 62: 4602-4610. Bartlett JG. 1992. Antibiotic-associated diarrhea. Clin Infect Dis. 15(4):573-81.Review. Bernet, M. F., D. Brassart, J. R. Neeser, and A. L. Servin. 1993. Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interaction. Appl. Environ. Microbiol. 59: 4121-4128. Bernet, M. F., D. Brassart, J. R. Neeser, and A. L. Servin. 1994. Lactobacillus acidophilus LA1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria. Gut. 35: 483-489. Brandtzaeg P, Kiyono H, Pabst R and Russell MW. 2008. Terminology: nomenclature of mucosa-associated lymphoid tissue. Mucosal Immunology 1(1); 31-37. Brito J. R., M. Hinton, C. R. Stokes, and G. R. Pearson. 1993. The humoral and cell mediated immune response of young chicks to Salmonella typhimurium and S. Kedougou. Br. Vet. J. 149: 225-34. Brooker, B. E., and R. Fuller. 1975. Adhesion of lactobacilli to the chicken crop epithelium. J. Ultrastruct. Res. 52:21-31. Chapman and Hall, London.McIntosh, G. H. 1996. Probiotics and colon cancer prevention. Asia Pac. J. Clin. Nutr.5:48-52. Chen T, Isomäki P, Rimpiläinen M, Toivanen P. 1999. Human cytokine responses induced by gram-positive cell walls of normal intestinal microbiota. Clin Exp Immunol. 118(2):261-7. Chiu, H. H., Tsai, C. C., Hsih, H. Y., Tsen, H. Y. 2007. Screening from pickled vegetables the potential probiotic strains of lactic acid bacteria able to inhibit the Salmonella invasion in mice. J. Appl. Microbiol. 104: 605-612. Chung, G. T. and Frost, A. J. 1970. The chemotherapy of experimental Salmonella choleraesuis infetion in mice. J. Med. Microbiol. 3: 303-311. Coconnier, M. H., M. F. Bernet, G. Chauviere, and A. L. Servin. 1993a. Adhering heat-killed human Lactobacillus acidophilus, strain LB, inhibits the process of pathogenicity of diarrhoeagenic bacteria in cultured human intestinal cells. J. Diarrhoeal Dis. Res. 11: 235-242. Coconnier, M. H., M. F. Bernet, S. Kerneis, G. Chauviere, J. Fourniat, and A. L. Servin. 1993b. Inhibition of adhesion of enteroinvasive pathogens to human intestinal Caco-2 cells by Lactobacillus acidophilus strain LB decreases bacterial invasion. FEMS Microbiol. Lett. 110: 229-306. Coconnier, M.H., Lievin, V., Lorrot, M., Servin, A.L. 2000. Antagonistic ctivity of Lactobacillus acidophilus LB against intracellular Salmonella enterica serovartyphimurium infecting human enterocyte-like Caco-2 / TC-7 cells. Appl. Environ.Microbiol. 66: 1152-7. Cole, C. B., R. Fuller, A. K. Mallet, and I. R. Rowland. 1985. The influence of the host on expression of intestinal microbial enzyme activities involved in metabolism of foreign compounds. J. Appl. Bacteriol. 59: 549-533. Collins MD and Gibson GR. 1999. Probiotics, prebiotics, and synbiotics: approaches for modulating the microbial ecology of the gut. American Society for Clinical Nutrition 69 (suppl): 1052S–1057S. Collington, G. K., D. S. Parker, and D. G. Armstrong. 1990. The influence of inclusion of either an antibiotic or probiotic in the diet on the development of digestive enzyme activity in the pig. Br. J. Nutr. 64: 59-70. Crociani J, Grill JP, Huppert M, Ballongue J. 1995. Adhesion of different bifidobacteria strains to human enterocyte-like Caco-2 cells and comparison with in vivo study. Lett Appl Microbiol. 21(3):146-8. Denter and Bisping. 1994. Formation of B-vitamins by bacteria during the soaking process of soybeans for temper fermentation. Int. J. Food Microbiol. 22(1): 23-31. Edens, F. W., C. R. Parkhurst, L. A. Casas, and W. J. Dobrogosz. 1997. Principles of ex ovo competitive exclusion and in ovo administration of Lactobacillus reuteri. Poultry Sci. 76: 179-196. Edelman, S., S. Leskela, E. Ron, J. Apajalahti, and T. K. Korhonen. 2003. In vitro adhesion of an avian pathogenic Escherichia coli O78 strain to surfaces of the chicken intestinal tract and to ileal mucus. Vet. Microbiol. 91: 41-56. Edelman, S., B. Westerlund-Wikstrom, S. Leskela, H. Kettunen, N.Rautonen, J. Apajalahti, and T. K. Apajalahti. 2002. In Vitro adhesion specificity of indigenous lactobacilli within the avian intestinal tract.Appl. Environ. Microbiol. 68: 5155-5159. Erickson KL and Hubbard NE. 2000. Symposium: Probiotic Bacteria: Implications for Human Health. American Society for Nutritional Sciences 130(suppl); 403S-409S. Eswarappa SM, Janice J, Nagarajan AG, Balasundaram SV, Karnam G, et al. 2008. Differentially Evolved Genes of Salmonella Pathogenicity Islands: Insights into the Mechanism of Host Specificity in Salmonella. PLoS ONE 3(12):1-12 Ewing, W. N., and D. J. A. Cole. 1994. The use of antibiotics. In: The living gut. pp. 75-89. Redwood. Books, Trowbridge, Wiltshire. England. Fuller, R. 1989. Probiotic in man and animals. J. Appl. Bacteriol. 66: 365-378. Fuller, R. 1992. Probiotics: The Scientific Basis. Chapman and Hall, London.Gadelle, D., P. Raibaud, and E. Sacquet. 1985. β-glucuronidase activities of intestinal bacterial determined both in vitro and in vivo rate. Appl. Microbiol. 49: 682-685. Fuller, R., and B. E. Brooker. 1973. Ecological studies on Lactobacillus flora associated with the crop epithelium of the fowl. J. Appl. Bacteriol.36:131-139. Fuller, R. 1975. Nature of the determinant responsible for the adhesion of lactobacilli to chicken crop epithelial cells. J. Gen. Microbiol.87:245-250. Gadelle, D., P. Raibaud, and E. Sacquet. 1985. β-glucuronidase activities of intestinal bacterial determined both in vitro and in vivo rate. Appl. Microbiol. 49: 682-685. Gibson GR. and Roberfroid MB. 1999. Dietary Modulation of the Human Colonie Microbiota: Introducing the Concept of Prebiotics. American Institute of Nutrition ;1401-1412. Gill, H. S. 1998. Stimulation of the immune system by lactic cultures. Int. Dairy J. 8:535-544. Gopal, P.K., Prasad, J., Smart, J., Gill, H.S. 2001. In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Escherichia coli. Int. J. Food Microbiol. 67(3): 207-16. Gorbach, S. L. 1986. Function of the normal human microflora. Scand. J. Infect. Dis. 49: 17-30. Hauri, H.P., Sterchi, E.E., Bienz, D., Fransen, J.A.M., Marxer, A. 1985. Expression and intracellular transport to microvillus membrane hydrolases in human intestinal epithelial cells. J. Cellular Biology. 101(3):838-851. Havenaar, R., B. T. Brink, and J. H. J. Huis In’t Veld. 1992. Selection of strains for probiotics use. In: R, Fuller. (Ed.). Probiotics:The Scientific Basis. pp. 209-222. Hawkswort, G., B. S. Drasar, and M. J. Hill. 1971. Intestinal bacteria and the hydrolysis of glycosidic bonds. J. Med. Microbiol. 4: 451-459. Hirano, J., Yoshida, T., Sugiyama, T., Koide, N., Mori I., Yokochi T. 2003. The effect of Lactobacillus rhamnosus on enterohemorrhagic Escherichia coli infection of human intestinal cells in vitro. Microbiol. Immuno. 47 (6): 405-409. Hogenauer C, Hammer HF, Krejs GJ, Reisinger EC. 1998. Mechanisms and Hütt P, Shchepetova J, Lõivukene K, Kullisaar T, Mikelsaar M. 2006. Antagonistic activity of probiotic lactobacilli and bifidobacteria against entero- and uropathogens. J Appl Microbiol. 100(6):1324-32 Holzapfel, W.H., Haberer, P., Snel, J., Schillinger, U. and Huis, H.J. 1998. Overview of gut flora and ptobiotics. International Journal of Food Microbiology 41:85-101. Isolauri E, Kirjavainen PV, Salminen S. 2002. Probiotics: a role in the treatment of intestinal infection and inflammation? Gut. 50 Suppl 3:III54-9. Review. Jay, J.M., 1996. Modern Food Microbiology. 5th Edition, Chapman and Hall.Jiang, T., A. Mustapha, and D. A. Savaiano. 1996. Improvement of lactose digestion in humans by injection of unfermented milk containing Bifidobacterium longum. J.Dairy Sci. 79: 750-757. Jiang, T., A. Mustapha, and D. A. Savaiano. 1996. Improvement of lactose digestion in humans by injection of unfermented milk containing Bifidobacterium longum. J.Dairy Sci. 79: 750-757. Jin, L. Z., Y. W. Ho, N. Abdullah, and S. Jalaludin. 2000. Digestive and bacterial enzyme activities in broilers fed diets supplemented with Lactobacillus cultures. Poult. Sci. 79: 886-891. Johnson, T.R., Case, C.L.1995. Laboratory experiments in Microbiology 4th edition. The Benjamin / Cummings Publoshing Co.Inc.Khedkar N, Gordon DL, Wagner R, Dillehay GL , Martinez CJ, Bayer W and Brooks MH. 1993. The effect of fine-needle aspiration biopsy on the thyroid scan. Clin Nucl Med. 18(6):495-7. Khedkar N, Gordon DL, Wagner R, Dillehay GL , Martinez CJ, Bayer W and Brooks 45 MH. 1993. The effect of fine-needle aspiration biopsy on the thyroid scan. Clin Nucl Med. 18(6):495-7. Kizerwetter-Swida M, Binek M. 2009. "Protective effect of potentially probiotic Lactobacillus strain on infection with pathogenic bacteria in chickens." Pol J Vet Sci. 12(1): 15-20. Ko JS, Yang HR, Chang JY, Seo JK. 2007. Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-alpha. World J Gastroenterol. 13(13):1962-5. Koo, F. C. and Peterson, J. W. 1983. Cell-free extracts of Salmonella inhibit protein synthesis and cause cytotoxicity in eukaryotic cells. Toxicon. 21: 309-320. Koo, F. C., Peterson, J. W., Houston, C. W. and Molina, N. C. 1984. Pathogenesis of experimental salmonellosis: inhibition of protein synthesis by cytotoxin. Infect Immun. 43: 93-100.931-939. Lilly, D. M., and R. H. Stillwell. 1965. Probiotic: growth promoting factors produced by microorganisms. Science. 174: 747-748. Ljungh A. and Wadström T. 2006. Lactic Acid Bacteria as Probiotics. Curr Issues Intestinal Microbiol. 7: 73-90. Mathew, A.G., Saxton, A.M., Upchurch, W.G., Chattin, S. E. 1999. Multiple antibiotic resistance patterns of Escherichia coli isolates from swine farms. Appl. Environ.Microbiol. 65:2770-2. Mcdonald, L.C., Ming-tsao, C., Tsai-ling, L., Monto, H. 2001. The use of antibiotics critical to human medicine in food-producing animals in Taiwan. Infect. Immuno.34:97-102. Mcintosh, G. H. 1996. Probiotics and colon cancer prevention. Asia Pac. J. Clin. Nutr.5:48-52. Muriana, P. M. and T. R. Kiaenhammer. 1991. Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088. Appl. Environ. Microbiol. 57:114-121. Nauciel, C., and F. Espinasse-Maes. 1992. Role of gamma interferon and tumor necrosis factor alpha in resistance to Salmonella typhimurium infection. Infect. Immun. 60: 450-454. Ouwehand, A. C., P. V. Kirjavainen, C. Shortt, and S. Salminen. 1999. Probiotic: mechanisms and established effects. Int. Dairy. J. 9:43-52. O’sullivan, M. G., G. Thornton, G. C. Osullivan, and J. K. Collins. 1992. Probiotic bacteria: myth or reality. Trends food technol. 3: 309-314. Parker, R. B. 1974. Probiotic, the other half of the antibiotic story. Anim. Nutr. Health. 29: 4-8. Pascual M., Hugas M., Badiola J. i., Josep maria monfort and Margarita garriga.1999. Lactobacillus salivarius CTC2197 Prevents Salmonella enteritidis Colonization in Chickens. Applied and environmental microbiology 66: 4981–4986. Pedersen, K., and G. W. Tannock. 1989. Colonization of the porcine gastrointestinal tract by Lactobacilli. Appl. Environ. Microbiol. 55: 279-283. Perdigon, G., E. Vintint, S. Alvarez, M. Medina, and M. Medici. 1999. Study of the possible mechanism involved in the mucosalimmune system activation by lactic acid bacteria. J. Dairy Sci. 82: 1108-1114. Pinto, M., Appay, S., Simon-Assmann, P., Chevalier, G., Dracopoli, N., Fogh, J.,Zweibaum, A. 1982. Enterocytic differentiation of cultured human colon cancer cells by replacement of glucose by gasactose in the medium. Biol. Cell. 44:193-6. Pinto, M., Robine-Leon, S., Kedinger, M.D., Triadou, M.,Dussaulx, N.,Lacroix, E.,Simon-Assmann, B., Haffen, P., Fogh, J., Zweibaum, A. 1983. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol. Cell. 47:323-30. Rambaud, J. C., Y. Bouhnik, P. Marteau, P. Pochart. 1993. Manipulation of the human gut microflora. Proc. Nutr. Sci. 52: 357-366. Rammelsberg, M., and F. Radler. 1990. Antibacterial polypeptides of Lactobacillus species. J. Appl. Bacteriol. 69: 177-184. Reid G., 2001. Probiotic agents to protect the urogenital tract against infection. Am J ClinNutr. 73, 437S-443S. Revolledo L., C. S. A. Ferreira. J. P. Ferreira.2009. Prevention of Salmonella Typhimurium colonization and organ invasion by combination treatment in broiler chicks. Poultry Science 88: 734-743. Rodrigo Bibiloni, Pablo Fernando Pe¨rez,, Graciela Liliana De Antoni.1999. "Will a High Adhering Capacity in a Probiotic Strain Guarantee Exclusion of Pathogens from Intestinal Epithelia? ." Anaerobe 5: 519-524. Salminen, S., A. Ouwehand, Y. Benno, and Y. K. Lee. 1999. Probiotics: how should they be defined.Trends Food Sci. Technol. 10: 107-110. Sanders ME, Walker DC, Walker KM, Aoyama K, Klaenhammer TR.1996. Performance of commercial cultures in fluid milk applications. J Dairy Sci.79(6):943-55. Sarem, F., L. D. Sarem-Damerdji, and J. P. Nicolas. 1996. Comparison of the adherence of three Lactobacillus strains to Caco-2 and Int-407 human intestinal cell lines. Lett. Appl. Microbiol. 22: 439-442. Sarrela, M., Lähteenmäki, L., Crittenden, R., Salminen, S. and Mattila-Sandholm, T.2002. Gut bacteria and health foods ¾ the European perspective. International Journal of Food Microbiology 78:99-117.7. Sartor, R.B. 2005. Probiotic therapy of intestinal inflammation and infections. Curr Opin Gastroenterol. 21(1):44-50. Savage, D. C. 1983. Mechanisms by which indigenous microorganism colonize gastrointestinal epithelial surfaces. Prog. Fd. Nutr. Sci. 7: 65-75. Scheinbach, S. 1998. Probiotics: Functionality and commercial status. Biotechnol. Adv.16: 581-608. Schleifer, J. H. 1985. A review of the efficacy and mechanism of competitive exclusion for the control of Salmonella in poultry. World’s Poult. Sci. J. 41:72-83. Sieo, C. C., N. Abdullah, W. S. Tan, and Y. W. Ho. 2005. Influence of β-glucanase-producing Lactobacillus strains on intestinal characteristics and feed passage rate of broiler chickens. Poult. Sci. 84: 734-741. Soerjadi, A. S., S. M. Stehman, G. H. Snoeyenbos, O. M. Weinack, and C.F. Smyser. 1981. The influence of Lactobacilli on the competitive exclusion of Salmonella paratyphoid in chickens. Avian Dis. 25:1027-1033. Sperti, G. S. 1971. Probiotics. West Point, CT: Avi Publishing Co. Sreekumar, O., and A. Hosono. 2000. Immediate effect of Lactobacillus acidophilus on the intestinal flora and fecal enzymes of rats and the in vitro inhibition of Escherichia coli in coculture. J Dairy Sci. 83: Stern, N. J., Cox, N. A., Bailey, J. S., Berrang, M. E. and Musgrove,M. T. 2001. Comparison of mucosal competitive exclusion and competitive exclusion treatment to reduce Salmonella and Campylobacter spp. colonization in broiler chickens. Poult. Sci. 80: 156-160. Thorne, G. M., Deneke, C. F., and Gorbach, S. L. 1979. Hemagglutination and adhesiveness of toxigenic Escherichia coli isolated from humans. Infect. Immun. 23(3): 690-699. Tsai, C.C, Hsih, H.Y, Huang, L.F., Lin C.C., Tsen H.Y. 2004. Antagonistic activity against Helicobacter pylori infection in vitro by a strains of Enterococcus faecium TM39. Int. J. Food Microbiol. 96(1):1– 12. Vesa,T. H., P. R. Marteau, F. B. Breat, M. C. Boutron-Rualt, and J. C. Rambaud. 1997.Raising milk energy content retards gastric emptying of lactose in lactose-intolerant humans with little effect on lactose digestion. J. Nutr. 127: 2316-2320. Weinack, O. M., G. H. Snoeyenbos, C. F. Smyser, and A. S. Soerjadi. 1981.Competitive exclusion of intestinal colonization of Escherichia coli in chicks. Avian Dis. 25: 696-705. Weinack, O. M., G. H. Snoeyenbos, C. F. Smyser, and A. S. Soerjadi. 1982.Reciprocal competitive exclusion of salmonella and Escherichia coli by native intestinal microflora of the chicken and turkey. Avian Dis.26:585-595. Yamano, T., Iino, H., Takada, M., Blum, S., Rochat, F., Fukushima, Y. 2006. Improvement of the human intestinal flora by ingestion of the probiotic strain Lactobacillus johnsonii La1. Br J Nutr. 95(2):303-12 Zavaglia, A.G., Kociubinski, G., Perez, P., de Antoni, G. 1998. Isolation and characterization of Bifidobacterium strains for probiotic formulation. J. Food Protect. 61: 865-73.
摘要: The objective of this study was to evaluate the possibility to use a Lactobacillus acidophilus strain as feed supplement and antibiotic use. This strains, ie, LAP5, was able to inhibit 17 Salmonella strains tested. Also, it demonstrated high adherence to human intestine epithelium cells Caco-2 as well as chicken crop epithelium cells. When three days aged chicken were fed with strain LAP5 200μl (109 cfu/mL ) continuously for 7 days and afterwards, challenged with Salmonella 200μl (109 cfu/mL ) for six days, results showed that Salmonella cells invaded in chicken liver and spleen were about 2 and 5 log CFU/g organ respectively. For LAP5 fed chickens, Salmonella cells invaded in liver and spleen reduced 1~2 log CFU/g organ (P<0.05) . Since LAP5 strain was able to inhibit the growth of pathogenic bacteria, with adherence and able to reduce the Salmonella infection through competitive expelling effect, the strain LAP5 could be used for probiotic use.
本研究目的在評估乳酸桿菌 Lactobacillus acidophilus LAP5 作為益生菌與取代抗生素用於雞隻飼養之可行性。於體外試驗中, LAP5 對本實驗 17 株沙門氏菌皆具抑菌效果。吸附試驗中以 LAP5 對雞隻嗉囊上皮細胞、腸道上皮及人類腸道上皮細胞株 Caco-2 具強吸附力,故以 LAP5 作為本研究之試驗菌株。於動物試驗中,鷄隻於三日齡時口投 LAP5 菌株 200 μl ( 109 cfu/mL )連續餵食七天,於 9日齡口投感染沙門氏菌菌液 200 μl (109 cfu/mL ) 連續餵食六天。結果顯示,感染後的雞隻肝臟與脾臟之菌數約達到 2-5 log cfu/g,餵食 LAP5 7 天後,雞隻肝臟與脾臟之菌數可下降 1-2 log cfu/g,且脾臟的保護效果高於肝臟 (P<0.05)。綜上所述, LAP5 菌株具有抑菌、吸附雞隻嗉囊上皮、雞隻腸道上皮細胞及人類腸道上皮細胞株 Caco-2 細胞之能力,且對沙門氏菌亦有競爭排除之效果,因此預期可作為益生菌之候選菌株。
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